Anti cd40 clone fgk4

Manufactured by BioXCell

The Anti-CD40 (clone FGK4.5) is a monoclonal antibody that binds to the CD40 antigen. CD40 is a cell surface receptor that plays a critical role in the immune response. The Anti-CD40 (clone FGK4.5) can be used for research purposes to study the function and signaling of the CD40 receptor.

Automatically generated - may contain errors

Lab products found in correlation

Ovalbumin (ova), by Merck Group (2 mentions) Ml rr s2 cda, by MedChemExpress (1 mentions) Sarrp 200, by Xstrahl (1 mentions) Tnf α, by BioLegend (1 mentions) Poly 1 c, by Merck Group (1 mentions) Ovalbumin (ova), by Thermo Fisher Scientific (1 mentions) Pierce lal chromogenic endotoxin quantitation kit, by Thermo Fisher Scientific (1 mentions) Ovalbumin (ova), by InvivoGen (1 mentions) Lsr 2 cytometer, by BD (1 mentions) Goat anti mouse igm f ab 2, by Jackson ImmunoResearch (1 mentions) Control rat igg, by Merck Group (1 mentions) Poly 1 c, by GE Healthcare (1 mentions) Poly 1 c, by InvivoGen (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)

Close spelling variants of this product

Anti-mouse CD40 (clone: FGK4.5/GFK45) Anti-CD40 antibody (clone FGK4.5; Anti-CD40 agonist (clone FGK4.5 Anti-CD40 (FGK4.5, Clone FGK4.5 Anti-CD40

9 protocols using anti cd40 clone fgk4

Combination Immunotherapy and Radiation for Pancreatic Cancer

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice received 30 μg of Flt3L (CDX-301, Celldex) i.p. every day for 9 days as previously published (Salmon et al., 2016 (link)). Mice received 100 μg of anti-CD40 (clone FGK4.5, BioXCell) i.p. every 5 days starting concurrent with other treatment upon palpation and 1^st ultrasound measure. Certain mice received 25 μg of STING agonist (ML-RR-S2 CDA, MedChemExpress) intratumorally every 4 days starting concurrent with other treatment upon palpation and 1^st ultrasound measure.
Mice received Radiation (RT) as three daily fractionated doses (8 Gy x 3) using the Small Animal Radiation Research Platform (SARRP200, XStrahl Life Sciences). Mice were injected i.p. with an iodine contrast agent (2100 mg/kg) before being placed on the irradiation platform one at a time under isoflurane anesthesia. Conebeam computed tomography (CT) imaging was performed for each individual mouse to pinpoint the pancreas, images were imported into Muriplan and used to select an isocenter. The tumor was then irradiated using anterior-posterior-opposed beams using the 10mm x 10mm collimator at a dose rate of 3.9 Gy/min. Mice were monitored over 2 weeks for signs of radiation sickness or weight-loss. DietGel recovery gel was provided for 14 day window immediately following radiation therapy in survival studies.

Hegde S., Krisnawan V.E., Herzog B.H., Zuo C., Breden M.A., Knolhoff B.L., Hogg G.D., Tang J.P., Baer J.M., Mpoy C., Lee K.B., Alexander K.A., Rogers B.E., Murphy K.M., Hawkins W.G., Fields R.C., DeSelm C.J., Schwarz J.K, & DeNardo D.G. (2020). Dendritic cell paucity leads to dysfunctional immune surveillance in pancreatic cancer. Cancer cell, 37(3), 289-307.e9.

+ Open protocol

+ Expand

Antitumor Immunotherapy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tumor-bearing mice were injected intratumorally twice, 2 days apart, with 100 μg anti-CD40 (clone FGK4.5; BioXCell), 0.2 μg TNFα (BioLegend), and 200 μg/mouse anti-TRP1 antibody (clone TA99; BioXCell).

Gutwillig A., Santana-Magal N., Farhat-Younis L., Rasoulouniriana D., Madi A., Luxenburg C., Cohen J., Padmanabhan K., Shomron N., Shapira G., Gleiberman A., Parikh R., Levy C., Feinmesser M., Hershkovitz D., Zemser-Werner V., Zlotnik O., Kroon S., Hardt W.D., Debets R., Reticker-Flynn N.E., Rider P, & Carmi Y. (2022). Transient cell-in-cell formation underlies tumor relapse and resistance to immunotherapy. eLife, 11, e80315.

+ Open protocol

+ Expand

Adoptive Transfer of OT1 T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

OT1 T cells were isolated from whole splenocytes by CD8-negative magnetic selection (Biolegend) and 10⁶ cells were adoptively transferred, unless otherwise noted, into CD45-congenic recipient mice by tail vein injection. The following day 250–500μg of depleting antibody was delivered intraperitoneally. For subunit-vaccinations, 100μg whole ovalbumin (Sigma), 50μg poly(I:C) (Sigma), and 50μg anti-CD40 (clone FGK4.5, made in house or from BioXCell) suspended in PBS was given intravenously and assessed 7 days later unless otherwise stated. For infectious challenge, 10⁷ PFU of Vaccinia virus expressing ovalbumin was given intravenously and assessed 5 days later unless otherwise stated. Spleens and lymph nodes harvested were macerated with glass slides, RBC lysed with ACK buffer, and stained with fluorochrome-conjugated antibodies to determine phenotype of transferred OT1 T cells.

Cross E.W., Blain T.J., Mathew D, & Kedl R.M. (2019). Anti-CD8 monoclonal antibody-mediated depletion alters the phenotype and behavior of surviving CD8+ T cells. PLoS ONE, 14(2), e0211446.

+ Open protocol

+ Expand

Ovalbumin Immunization with Adjuvants

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ovalbumin (Catalog number A5503, Sigma-Aldrich, St. Louis, MO) was decontaminated of lipopolysaccharide using a Triton X-114 detoxification method^{60 (link)} and tested with Pierce LAL chromogenic endotoxin quantitation kit (catalog number 88282, Thermo Fisher Scientific, Waltham, MA). Ovalbumin was labeled with Alexafluor 488 using Alexafluor 488 succimidyl ester labeling system (Catalog number A20100, Thermo Fisher Scientific, Waltham, MA). Ovalbumin was used in combination with poly(I:C) (catalog number VAC-PIC, Invivogen, San Diego, CA), and anti-CD40 (clone FGK4.5, BioXCell, West Lebanon, NH)^{61 (link),62 (link)}. For pathogen challenge, female C57BL/6 mice were injected with either 1 × 10⁷ pfu/mouse of vaccinia virus western reserve strain expressing the Ovalbumin protein (VV-ova) intraperitoneally or 1 × 10⁴ pfu/footpad^{24 (link)} using the vaccinia virus western reserve strain with Ovalbumin labeled with Alexafluor 488. For anti-PDPN injection purified clone 8.1.1⁴ (catalog number 127402, Biolegend, San Diego CA) was injected at 100 μg intravenously 4 h before ova-488 and 72 h later.

Kedl R.M., Lindsay R.S., Finlon J.M., Lucas E.D., Friedman R.S, & Tamburini B.A. (2017). Migratory dendritic cells acquire and present lymphatic endothelial cell-archived antigens during lymph node contraction. Nature Communications, 8, 2034.

+ Open protocol

+ Expand

B Cell Migration Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

B cells were enriched from spleens of cocaged littermate males by depletion using biotinylated antibodies (anti-CD43, anti-TER119, anti-CD11c, anti-TCRβ, anti-CD4, and anti-CD8) and streptavidin-conjugated beads (EasySep Streptavidin RapidSpheres). B cells were activated with 10 µg ⋅mL^--1 F(ab′)2 goat anti-mouse IgM (Jackson Immunoresearch) and 10 µg ⋅mL^--1 anti-CD40 (clone FGK4.5; Bio X Cell) in complete RPMI for 60 h. B cells were collected, washed in prewarmed migration medium (RPMI containing 0.5% fatty acid-free BSA, 10 mM Hepes, and 50 IU penicillin/streptomycin), and rested in migration medium at 37 ^∘C for 1 h. B cells (200 µL, 1 × 10⁶cells) were then added to transwells (5-µm pore; Corning Costar no. 93421) with the indicated chemokines in migration media (600 µL) in the bottom chamber and allowed to migrate for 3 h at 37 ^∘C and 5% CO₂. Migrated cells were then collected from the bottom chamber, stained for viable B220⁺ cells, and collected on a BD LSR II cytometer for a fixed time under constant flow rate and normalized to an “input” well.

Wolf E.W., Howard Z.P., Duan L., Tam H., Xu Y, & Cyster J.G. (2022). GPR174 signals via Gαs to control a CD86-containing gene expression program in B cells. Proceedings of the National Academy of Sciences of the United States of America, 119(23), e2201794119.

+ Open protocol

+ Expand

Adoptive Transfer of Tumor-Specific CD8+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Irradiation-conditioned mice were administered 4 Gy WBI on day −1 using a ⁶⁰Co Gammacell irradiator (Nordion International) or an X-RAD 320ix biological x-ray irradiator (Precision X-Ray Inc.). Anti-CD40- and control-conditioned mice received 100 μg purified anti-CD40 (clone FGK45, BioXcell) or control rat IgG (Sigma) on days −1 and +1 by intraperitoneal (i.p.) injection. Whole-cell populations were recovered from spleens and axillary, brachial, superficial cervical, mesenteric, inguinal, and lumbar lymph nodes [35 (link)] of TCR-IV donor mice. CD8⁺ cells were enriched by autoMACS magnetic sorting using the manufacturer’s recommendations (Miltenyi Biotec), resulting in approximately 90% pure CD8⁺TCR-IV⁺ T cells. 1 × 10⁶ naïve TCR- IV T cells were administered intravenously in 200 μL PBS on day 0 of the experiments.

Cozza E.M., Cooper T.K., Budgeon L.R., Christensen N.D, & Schell T.D. (2014). Protection from tumor recurrence following adoptive immunotherapy varies with host conditioning regimen despite initial regression of autochthonous murine brain tumors. Cancer immunology, immunotherapy : CII, 64(3), 325-336.

+ Open protocol

+ Expand

Generating Antigen-Specific T Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Draining lymph nodes and spleen were used to gather activated T cells. Ovalbumin (Sigma-Aldrich, St. Louis, MO) or HSVgB (produced in the lab using baculovirus expression) were decontaminated of lipopolysaccharide (LPS) using a Triton X-114 LPS detoxification method ^{70 (link)} were used in combination with polyI:C (GE Healthcare), pam3cys [N-palmitoyl-S-2,3-bis(palmitoyloxy)-(2R,S)-propyl]-(R)-cysteine-(S)serine-(S)lysine 4] (InvivoGen, San Diego, CA), anti-CD40 (clone FGK4.5, BioXCell, West Lebanon, NH) or the TLR7a agonist, 3M012, conjugated to Ovalbumin ^{32 (link),33 (link)}. For pathogen challenge, female B6 mice were injected with either 1×10⁷ pfu/mouse of Vaccinia Virus Western Reserve Strain expressing the Ovalbumin protein (VV-ova) or 1×10⁵ Listeria Monocytogenes (Lm) expressing whole Ovalbumin protein (Lm-ova).

Tamburini B.A., Burchill M.A, & Kedl R.M. (2014). Antigen capture and archiving by lymphatic endothelial cells following vaccination or viral infection. Nature communications, 5, 3989.

+ Open protocol

+ Expand

Intravenous Delivery of MVA Recombinants

Check if the same lab product or an alternative is used in the 5 most similar protocols

Intravenous (i.v.) injections were given into a lateral tail vein with a total volume of 200 μl containing 5 × 10⁷ 50% tissue culture infective dose (TCID₅₀) of the respective MVA recombinants. Where noted, anti‐CD70 antibody (clone FR70, Bio X Cell) was injected i.v. at the indicated doses and time‐points. No differences were observed between the different FR70 doses used. For in vivo activation of splenic APC, 100 μg poly(I:C) (pIC, high molecular weight, Thermo Fisher Scientific, Waltham, MA) + 50 μg anti‐CD40 (clone FGK4.5, Bio X Cell, West Lebanon, NH) were injected i.v. For ectromelia virus (ECTV, strain Moscow) infection, MHC II^−/− mice were anaesthetized with ketamine/xylamine and virus (1 × 10⁵ TCID₅₀) was applied by intranasal drop‐wise installation in a total volume of 50 μl. The health status of infected mice was monitored twice daily.

Bathke B., Pätzold J., Kassub R., Giessel R., Lämmermann K., Hinterberger M., Brinkmann K., Chaplin P., Suter M., Hochrein H, & Lauterbach H. (2018). CD70 encoded by modified vaccinia virus Ankara enhances CD8 T‐cell‐dependent protective immunity in MHC class II‐deficient mice. Immunology, 154(2), 285-297.

+ Open protocol

+ Expand

SARS-CoV-2 RBD Protein Immunization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments involving mice were conducted following protocols approved by the University of Colorado Institutional Animal Care and Use Committee (IACUC) according to guidelines provided by the Association for Assessment and Accreditation of Laboratory Animal Care. C57BL/6 mice were obtained from the Jackson Laboratory and were subsequently bred in specific-pathogen-free facilities at the University of Colorado Anschutz Medical Campus. Experiments were performed in 6–12-week-old female mice. Mice were immunized via tail-vein injection with 100 μg or 200 μg, of SARS-CoV-2 spike RBD protein plus adjuvant. SARS-CoV-2 RBD protein (Wuhan-Hu-1; GenBank: MT380724.1) was expressed by transfection of Expi293 cells with a His-tagged vector (a gift from F. Krammer, Icahn School of Medicine at Mount Sinai, New York, NY)(11 (link)) and subsequently purified from cell culture supernatants by the University of Colorado Cell Technologies Shared Resource. Immunizations were adjuvanted with 40 μg poly(I:C) (Invivogen), and 40 μg anti-CD40 (clone FGK4.5, BioXCell). Vaccines were made immediately prior to immunization and injected in a total volume of 200 μl.

Davenport B.J., Morrison T.E., Kedl R.M, & Klarquist J. (2021). Conserved and novel mouse CD8 T cell epitopes within SARS-CoV-2 spike RBD protein identified following subunit vaccination. Journal of immunology (Baltimore, Md. : 1950), 206(11), 2503-2507.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!