The cell suspension was seeded in 96-well plates (3,000 cells/well) and pre-incubated for 24 h with RPMI-1640 media containing 10% FCS, penicillin and streptomycin. Cells were subsequently treated with si-AMACR or si-CON for 0, 24, 48, or 72 h, respectively. Cells were also treated with Ad-CON, Ad-miR200c, or Ad-miR200c + pcDNA3.1-AMACR for 0, 24 or 48 h, respectively. Subsequently, 10 µl CCK-8 (Dojindo Molecular Technologies, Inc.) was added to each well and incubated for 2 h at 37°C. The absorbance was examined at 450 nm using a scanning multi-well spectrophotometer (Thermo Fisher Scientific, Inc.). The proliferation index (PI) was calculated as PI=[(Asample-Ablank)/(Acontrol-Ablank)] ×100%.
Scanning multiwell spectrophotometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Scanning Multiwell Spectrophotometer is a laboratory instrument designed to measure the absorbance of samples in multi-well plates. It provides a rapid and accurate way to analyze multiple samples simultaneously. The core function of this device is to quantify the optical density of substances within the wells, which can be used to determine various properties of the samples, such as concentration or chemical composition.
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Lab products found in correlation
6 protocols using scanning multiwell spectrophotometer
1
Cell Proliferation Assay with siRNA and Adenovirus
2
Cell Proliferation Assay via BrdU Incorporation
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Cells are cultured in the presence of the respective test substances in a 96-well MP at 37 °C for a certain period of time (1–5 days, depending on the individual assay system); subsequently, BrdU is added to the cells and the cells are reincubated (usually 2–24 h). During this labeling period, the pyrimidine analogue BrdU is incorporated in place of thymidine into the DNA of proliferating cells; After removing the culture medium the cells are fixed and the DNA is denatured in one step by adding FixDenat (Roche, CH, Basel, Switzerland) (the denaturation of the DNA is necessary to improve the accessibility of the incorporated BrdU for detection by the antibody); The anti-BrdU-POD binds to the BrdU incorporated in newly synthesized, cellular DNA; the immune complexes are detected by the subsequent substrate reaction; and the reaction product is quantified by measuring the absorbance at the respective wavelength using a scanning multiwell spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). The developed color and thereby the absorbance values directly correlate to the amount of DNA synthesis and thereby to the number of proliferating cells in the respective microcultures.
3
Cytotoxicity evaluation of Korean Red Ginseng
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To detect the cytotoxicity effect of KRG, cells were seeded (1 × 105 cells/mL) and treated with 2, 4, 6, 8, 10, 20, 40, 80, or 100 µg/mL of KRG. After 48 h, the cells were cocultured with MTT solution. After a four-hour-long incubation period, the transformed formazan crystals were dissolved in DMSO. The formazan product was measured at an absorbance of 540 nm under a scanning multi-well spectrophotometer (Thermo Fisher Scientific).
4
Cell Viability Assay Protocol
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The transfected cell suspension (3,000 cells/well, 100 μL) was dispensed in 96-well plates and incubated in an incubator for 24, 48, and 72 h (humidified atmosphere, 37°C, 5% CO2). Subsequently, the cells were then incubated with 10 μL of CCK8 (Dojindo Laboratories, Kumamoto, Japan) per well at 37°C for 4 h in the incubator. The absorbance at 460 nm (A460) was then examined using a scanning multiwell spectrophotometer (Thermo Scientific).
5
CCK-8 Assay for 5FU Cell Viability
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Cell viability was determined using a CCK-8 kit (HY-K0301; MCE). Briefly, cells (2500/well) were cultured in 96-well plates for 72 h. Ten microliter of CCK-8 (Dojindo Laboratories) was added to each well, and the cells were incubated for an additional 3 h at 37 °C. The absorbance was then measured at 450 nm using a scanning multiwell spectrophotometer (Thermo Scientific). Cells were treated with various concentrations of 5FU for 72 h. The 50% inhibitory concentration (IC50) value was calculated.
6
Cell Proliferation Assay with CCK8
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Suspension of transfected cells was dispensed into 96-well plates (1,000 cells/well, 100 μL) and then incubated for 1 to 7 days (humidified atmosphere, 37°C, 5% CO2). Thereafter, 10 μL of CCK8 (Dojindo Laboratories, Kumamoto, Japan) was added to each well, and the cells were incubated for an additional 4 h at 37°C. The absorbance at 460 nm (A460) was then assayed using a scanning multiwell spectrophotometer (Thermo Scientific).
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