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Mouse anti il 8

Manufactured by Abcam
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Mouse-anti IL-8 is a primary antibody that recognizes the cytokine interleukin-8 (IL-8) in mouse samples. It can be used for various research applications.

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Lab products found in correlation

Goat anti mouse cy3, by Abcam (1 mentions) Ab18672, by Abcam (1 mentions)

Close spelling variants of this product

Anti-IL-8 Mouse anti

2 protocols using mouse anti il 8

1

Immunofluorescent Detection of Cytokines in OCT Biopsy

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OCT-embedded biopsy samples were cryosectioned and adhered to positively charged slides. Slides were fixed in 100% ethanol, blocked with 1% BSA and 5% milk, and stained with either mouse-anti IL-8 or rabbit-anti IL-6 primary antibodies (Abcam). Goat anti-mouse Cy3 or goat anti-rabbit Cy5 antibodies (Abcam) were used for secondary detection. Control sections were prepared by incubating tissue without primary antibody to confirm the absence of background staining. Images were captured with a fluorescent Zeiss Axio Imager microscope (Jena, Germany).
Prindeze N.J., Ardanuy J.G., Carney B.C., Moffatt L.T, & Shupp J.W. (2019). Photobiomodulation Elicits a Differential Cytokine Response in a Cultured Analogue of Human Skin. Eplasty, 19, e3.
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2

Immunostaining of IL-8 and Neutrophils in FFPE Tissue

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To confirm sequencing results, 5-μm FFPE sections from 10 GCA/VZV-positive TAs and 10 control/VZV-negative TAs were immunostained for interleukin (IL)-8 and neutrophils (CD15). After deparaffinization, antigens were retrieved in citrate buffer at 95°C for 10 minutes. Slides were immunostained as described1 (link) using primary antibodies against IL-8 (mouse anti-IL-8; 1:500 dilution; Abcam, Cambridge, MA; catalog #ab18672) and CD15 (mouse anti-CD15; 1:50 dilution; Abcam; catalog #ab188610). IL-8 and CD15 positive control was normal human tonsil. Slides were imaged by light microscopy.
Bubak A.N., Mescher T., Mariani M., Frietze S.E., Hassell JE J.r., Niemeyer C.S., Como C.N., Burnet A.M., Subramanian P.S., Cohrs R.J., Mahalingam R, & Nagel M.A. (2021). Targeted RNA Sequencing of Formalin-Fixed, Paraffin-Embedded Temporal Arteries From Giant Cell Arteritis Cases Reveals Viral Signatures. Neurology® Neuroimmunology & Neuroinflammation, 8(6), e0178.
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