Irdye 800 conjugated anti rabbit igg

Dopamine hydrochloride, 3–4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) were all purchased from Sigma (St Louis, MO). Halt protease and phosphatase inhibitor cocktail was obtained from Thermo Fisher (Waltham, MA). Bradford assay reagent and Western blotting buffers were purchased from Bio-Rad (Hercules, CA). Anti-4-hydroxynonenal antibody was purchased from R&D Systems (MAB3249, Minneapolis, MN (Ghosh et al., 2016 (link))), while anti-BDNF was purchased from Santa Cruz Biotechnology (sc-546, Dallas, TX). CREB and p-CREB (Ser133) antibodies were obtained from Cell signaling (9104, 87G3, Boston, MA (Jin et al., 2011 (link))). The anti-mouse and anti-rabbit secondary antibodies (Alexa Fluor 680 conjugated anti-mouse IgG and IRdye 800 conjugated anti-rabbit IgG) were purchased from Invitrogen and Rockland Inc., respectively.

Dopamine hydrochloride, 3-4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), 3,3′-diaminobenzidine (DAB), manganese chloride (MnCl₂), and hydrogen peroxide were all purchased from Sigma (St Louis, MO). Halt protease and phosphatase inhibitor cocktail was obtained from Thermo Fisher (Waltham, MA). Bradford assay reagent and Western blotting buffers were purchased from Bio-Rad Laboratories (Hercules, CA). Anti-4-hydoxynonenal (4-HNE) antibody was purchased from R&D Systems (Minneapolis, MN). We purchased anti-IBA-1 antibodies from Wako Pure Chemical Industries (Richmond, VA) and Abcam (Cambridge, MA) for immunohistochemistry (IHC) and Western blot, respectively. Anti-tyrosine hydroxylase (TH) antibody was purchased from Millipore (Billerica, MA). Anti-oligomeric antibody (A11) and cell culture reagents were purchased from Invitrogen. The anti-mouse and anti-rabbit secondary antibodies (Alexa Fluor 680 conjugated anti-mouse IgG and IRDye 800 conjugated anti-rabbit IgG) were purchased from Invitrogen and Rockland Inc., respectively.