Proteose peptone, Page’s saline, and yeast extract were procured from HiMedia Laboratories (Mumbai, India). Sodium citrate dihydrate (C6H5Na3O7.2H2O), disodium phosphate (NaHPO4), sodium chloride (NaCl), calcium chloride (CaCl2), glucose, and phenylmethylsulphonyl fluoride (PMSF) were from Sigma Chemical Co. (St. Louis, MO, USA). Potassium dihydrogen phosphate (KH2PO4) and magnesium sulfate heptahydrate (MgSO4.7H2O) were procured from Labscan (Bangkok, Thailand). Trypan blue (0.4%) was obtained from Gibco BRL (Grand Island, NY, USA). All chemicals and medium components used were of analytical grade.
Proteose peptone
Manufactured by HiMedia
Sourced in India
Proteose peptone is a complex mixture of peptides and amino acids derived from the enzymatic digestion of proteins. It serves as a nutrient source for the growth and cultivation of various microorganisms in laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
Yeast extract, by HiMedia (5 mentions)
Sodium chloride (nacl), by HiMedia (2 mentions)
Ponddtox, by Novozymes (2 mentions)
Sodium thiosulphate, by HiMedia (2 mentions)
Sodium deoxycholate, by HiMedia (2 mentions)
Sodium lauryl sulphate, by HiMedia (2 mentions)
Potassium tellurite, by HiMedia (2 mentions)
Tcbs agar, by HiMedia (2 mentions)
D glucose anhydrous, by HiMedia (2 mentions)
Casein enzymic hydrolysate, by HiMedia (2 mentions)
Bile salt, by HiMedia (2 mentions)
Graphene oxide, by Merck Group (2 mentions)
Nutrient agar, by HiMedia (2 mentions)
Nutrient broth, by HiMedia (2 mentions)
Luria bertani broth, by HiMedia (2 mentions)
Oxgall, by HiMedia (2 mentions)
Sucrose, by HiMedia (2 mentions)
Sodium citrate, by HiMedia (2 mentions)
Phenylmethylsulphonyl fluoride pmsf, by Merck Group (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
5 protocols using proteose peptone
1
Microbial Culture Medium Composition
2
Fabrication of Asymmetric Membranes with Antimicrobial Properties
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Polyvinylidene uoride (MW = 573 KDa, Solef, Solvay, France), polyester fabric (Filtration Sciences Corporation, USA), N, N dimethyl formamide (Qualigen, India), D-(+)-Glucose anhydrous (Hi media, India), Graphene oxide (Sigma-Aldrich) and 2, 3, 5-Triphenyltetrazolium chloride (≥ 99.0% SIGMA, Life Science) were used to prepare the asymmetric membranes. Reverse osmosis treated water was used for the membrane preparation.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
3
Vibrio Strains Cultivation and Preservation
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Vibrio strains were bought from the Bioresource Collection and Research Center, Taiwan, including V. alginolyticus ATCC 17749, V. carchariae ATCC 35084, V. damsela ATCC 33536, V. harveyi ATCC 14126, V. parahaemolyticus ATCC 17802, V. pelagius ATCC 25916, and V. vulnificus BCRC15431. The Vibrio strains were maintained in Bacto Brain Heart Infusion medium (BHI; Becton-Dickinson Co, USA) supplemented with 3% NaCl (Panreac Quimica, France). For long-term preservation, bacteria were frozen at −80°C in BHI supplemented with 1% NaCl and 25% glycerol (Nihon Shiyaku, Japan). The strains were streaked onto modified seawater yeast extract (rich MSWYE) agar plates consisting of 23.4 g NaCl, 6.98 g MgSO4_7 H2O, and 0.75 g KCl in 1000 ml distilled water [18 (link)]. The pH was adjusted to 7.6 with 1 N NaOH, followed by the addition of 5.0 g of proteose peptone (HIMEDIA Lab, India), 3.0 g of yeast extract (HIMEDIA Lab, India), and 20.0 g of agar per liter.
4
Cultivation and Cyst Formation of Acanthamoeba Strains
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Acanthamoeba castellanii non-pathogenic strain (ATCC 30010) and
Acanthamoeba castellanii pathogenic strain (ATCC 50739) were kindly given by Asst. Prof. Dr. Rachasak Boonhok, Walailak University. Trophozoites were grown in 75 cm
2 tissue culture flasks in Peptone yeast extract glucose Broth (PYG) medium containing proteose peptone 0.75% (w/v), yeast extract 0.75% (w/v) and glucose 1.5% (w/v) (purchased from HiMedia Laboratories Pvt.Ltd., Mumbai, India), without shaking at 28°C as described previously
10 (link)
. For cysts, trophozoites were transferred from the PYG medium to the Neff’s encystment medium (NEM) containing 0.1 M KCl, 8 mM MgSO
4·7H
2O, 0.4 mM CaCl
2·2H
2O, 1 mM NaHCO
3, 20 mM ammediol (purchased from RCI Labscan Limited, Bangkok, Thailand) and were cultured in this medium for seven days to obtain mature cysts. After that, mature cysts were harvested and washed twice using 10 mL sterile phosphate-buffer saline (PBS).
+ Expand
Acanthamoeba castellanii non-pathogenic strain (ATCC 30010) and
Acanthamoeba castellanii pathogenic strain (ATCC 50739) were kindly given by Asst. Prof. Dr. Rachasak Boonhok, Walailak University. Trophozoites were grown in 75 cm
2 tissue culture flasks in Peptone yeast extract glucose Broth (PYG) medium containing proteose peptone 0.75% (w/v), yeast extract 0.75% (w/v) and glucose 1.5% (w/v) (purchased from HiMedia Laboratories Pvt.Ltd., Mumbai, India), without shaking at 28°C as described previously
10 (link)
. For cysts, trophozoites were transferred from the PYG medium to the Neff’s encystment medium (NEM) containing 0.1 M KCl, 8 mM MgSO
4·7H
2O, 0.4 mM CaCl
2·2H
2O, 1 mM NaHCO
3, 20 mM ammediol (purchased from RCI Labscan Limited, Bangkok, Thailand) and were cultured in this medium for seven days to obtain mature cysts. After that, mature cysts were harvested and washed twice using 10 mL sterile phosphate-buffer saline (PBS).
5
Fabrication of Asymmetric Membranes with Antimicrobial Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Polyvinylidene uoride (MW = 573 KDa, Solef, Solvay, France), polyester fabric (Filtration Sciences Corporation, USA), N, N dimethyl formamide (Qualigen, India), D-(+)-Glucose anhydrous (Hi media, India), Graphene oxide (Sigma-Aldrich) and 2, 3, 5-Triphenyltetrazolium chloride (≥ 99.0% SIGMA, Life Science) were used to prepare the asymmetric membranes. Reverse osmosis treated water was used for the membrane preparation.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
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