Cary 630 ftir

The equipment used for the study are Centrifuge (800D) from Gulfex medical and scientific, England; Spectrophotometer from Spectrum Lab and 52b New life medical instrument, England; Water Bath (OLS200) from Grant Instrument (Cambridge) LTD; Weighing Balance (Scout Pro Spo402) from Ohaus Corporation, Pine Brrok, NJ, USA; FTIR (Cary630) from Agilent Technology, Malaysia; Heater (HP139920-33) from Barnstead Thermolyne, England; Glucometer (CE0088) from Roche Diagnostic GmnH, Germany; and GC-MS from Agilent 19091S-433UI Agilent Technologies, United States.

FTIR (Cary630) from Agilent Technology, Malaysia, was used for the analysis, and the method of Griffiths and De-Haseth [28 ] was followed with slight modification. The instrument was powered on and waited until the initialization was completed. The sample holder was cleaned with tissue paper moistened with acetone. The spectrum software on the computer was set up to pop up “Scan and Instrument Setup” dialog. The sample name and the scan range limit (650–4500 cm⁻¹) were input.
The “background” icon was clicked to collect the background information of the sample holder before the sample was placed on the sample holder. The “monitor” icon on the “Scan and Instrument Setup” the dialog was activated, and then, the pressure arm was lowered down to the sample until a sound was heard and then “finish” button was activated. “Apply” and then “Start” icons were activated to collect the spectrum. Data of the spectrum obtained were processed by clicking on the “view” and then on the “label peaks” icons to select and label the spectrum with the desired or special peaks. The data were then saved as pdf files.

FTIR analysis of the plant sample was done using Cary 630 FTIR (Agilent Technologies). The plant samples from different durations were oven-dried at 80 °C for 48 h. The oven-dried plant samples were pulverized using pestle mortar. For the FTIR analysis, the pulverized sample was mixed with potassium bromide (KBr) followed by pellets formation using agate mortars. The absorbance spectrum was obtained for a range between 400 and 4000 cm⁻¹.

The surface morphology of the sample after the single nanosecond pulse laser processing was investigated by scanning electron microscopy (SEM, Phenom ProX, Phenon World, Eindhoven, The Netherlands). X-ray diffraction (XRD, D8 FOCUS, Bruker, Billerica, MA, USA), point energy-dispersive X-ray spectroscopy (EDS, Phenom ProX, Phenon World, Netherlands), and Fourier-transform infrared (FTIR, Cary 630 FTIR, Agilent Technologies, Santa Clara, CA, USA) were used to evaluate chemical compositions on the surface. The wettability on the laser-treated surface was measured by an optical contact angle and interface tension meter (SL200KB, Kino, Kailua Kona, HI, USA) with a 5-μL volume of deionized (DI) water droplets.

Infrared spectra were recorded using a Diamond ATR sampling block for a Cary 630 FTIR (Agilent Technologies, Santa Clara, CA, USA). The spectra of polymers were obtained from around 10 µL of liquid samples (50% in water). The spectra of NAs and dNAs were recorded with around 100 µg of freeze-dried samples. Briefly, the stored solution was centrifuged at 13,400 rpm for 5 min, suspended in 500 µL of MilliQ water, sonicated for 5 min, and freeze-dried overnight.

Graphene oxide was prepared and characterized following previous reports (Hummers and Offeman, 1958 (link)). GO (3 mg/mL) was prepared following a modified Hummers method and using a 7-day oxidation time. A 1.3 mg/mL nGO concentration was obtained from the GO suspension, by heating the GO suspension at 80°C for 24 h in a mixture of sulfuric and nitric acid (4:1 v/v). The resulting solution was neutralized, sonicated for about 3 h at 50°C, and purified by repeated dialysis (MWCO 15 kDa) in ultrapure water.
The shape and thickness of GO and nGO samples were characterized by tapping-mode atomic force microscopy (Bruker Dimension Icon AFM), Dynamic Light Scattering and Zeta Potential (Malvern Zetasizer Nano ZS90). Transmission electron microscopy (TEM) was performed in a JEOL – JEM2100 LaB6 HR operating at 200 kV. Samples for TEM were prepared by drop casting of nGO and GO dispersion on ultra-thin carbon film-coated copper grid, 300 mesh. Luminescence emission measurements were performed at room temperature on a Cary Eclipse, Agilent technology spectrofluorometer and a Varian Cary^® 50 UV-VIS system spectrophotometer.
Other techniques, including infrared spectroscopy (Agilent Technologies, Cary 630 FTIR), Raman spectroscopy (Renishaw RM2000, laser HeNe and 632.8 nm wavelength) were adopted to characterize the GO and nGO samples.

The Fourier transform infrared spectroscopy of Acl alone and with mixed pluronic at 298 K was noted in the range of 700 to 2500 cm⁻¹ by using Cary-630 FT-IR Agilent Technology. 2 ml solution of mixed pluronic, aceclofenac and combination of both were taken and their individual spectrums were recorded.