Inl191 blood flowmeter lcc

Assessment of dynamic vasoconstriction and vasodilatation of microcirculation following sympathetic stimulation will be performed to evaluate neurovascular autonomic function as previously described [18 (link)]. Briefly, assessment will be undertaken using laser Doppler surface probes (INL191 Blood FlowMeter LCC, ADInstruments, Castle Hill, Australia) following deep inspiration to stimulate a vasoconstrictory sympathetic response. The LDF technique utilizes a monochromatic coherent laser source on a fixed cutaneous region. The LDF probe will be attached at the middle phalanx of the fourth finger of the non-dominant hand with a circular strip. Processing and computation of the distribution of frequencies of backscattered light enables an approximation of small vessel blood perfusion. The electrical signal from the LDF probe will be transmitted to the PowerLab 4/26® analogue-to-digital converter (ADInstruments, Castle Hill, Australia), which generates the digitised representation of the microcirculation of the skin. Vasoconstrictory response (VCR) as well as the time required (sec) for the vessel to develop 50% constriction or 50% dilation using Δt50%down and Δt50%up values will be computed [18 (link)].

Assessment of dynamic vasoconstriction and vasodilatation of microcirculation following sympathetic stimulation will be performed to evaluate neurovascular autonomic function as previously described [18 (link)]. Briefly, assessment will be undertaken using laser Doppler surface probes (INL191 Blood FlowMeter LCC, ADInstruments, Castle Hill, Australia) following deep inspiration to stimulate a vasoconstrictory sympathetic response. The LDF technique utilizes a monochromatic coherent laser source on a fixed cutaneous region. The LDF probe will be attached at the middle phalanx of the fourth finger of the non-dominant hand with a circular strip. Processing and computation of the distribution of frequencies of backscattered light enables an approximation of small vessel blood perfusion. The electrical signal from the LDF probe will be transmitted to the PowerLab 4/26® analogue-to-digital converter (ADInstruments, Castle Hill, Australia), which generates the digitised representation of the microcirculation of the skin. Vasoconstrictory response (VCR) as well as the time required (sec) for the vessel to develop 50% constriction or 50% dilation using Δt50%down and Δt50%up values will be computed [18 (link)].