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4 protocols using luteolin 7 o glucoside

1

Antioxidant Activity of Plant Extracts

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The standard substances α-pinene (98%), sabinene (95%), p-cymene (99%), limonene (97%), linalool (97%), γ-terpinene (96%), (E)-β-caryophyllene (98.5%), germacrene D (90%), α-humulene (96%), caryophyllene oxide (95%) (98%), p-hydroxybenzoic acid (99%), caffeic acid (98%), o-hydroxybenzoic acid (99%), rosmarinic acid (97%), rutin (94%), AAPH (97%), and fluorescein (99%) were purchased from Sigma Aldrich (St. Louise, MO, USA), and luteolin-7-O-glucoside (98%), kaempferol-3-O-rutinoside (98%), and salvigenin (98%) were purchased from ChemFaces (Wuhan, China); vitexin (≥95%) was purchased from PhytoLab GmbH (Vestenbergsgreuth, Bavaria, Germany). Solvents and other reagents used in the extraction process, GC/FID grade dichloromethane, ammonium formate (99%), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), formic acid HPLC grade, potassium persulfate (99%), and absolute ethanol (96%) were purchased from Merck (Darmstadt, Germany); commercial ethanol (96%) was acquired from SUQUIN (Bucaramanga, Santander, Colombia).
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2

Immune Checkpoint Inhibitor Screening Assays

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Caffeic acid (#CFN99190), luteolin (#CFN98784), luteolin 7-O-glucoside (#CFN98565), cosmosiin (#CFN98981), homoplantaginin (#CFN90344), apigenin (#CFN98843), and hispidulin (#CFN99491) were purchased from ChemFaces (Wuhan, China). PD-1/PD-L1 inhibitor screening assay kit (#72005), CTLA-4 (cytotoxic T-lymphocyte-associated protein 4)/CD80 inhibitor screening assay kit (#72009), and PD-L1-blocking antibody (#71213) were purchased from BPS Bioscience (San Diego, CA, USA). Small molecule PD-L1 inhibitor C1 (BMS-1, # T3655) was purchased from TargetMol (Wellesley Hills, MA, USA). Anti-PD-1 antibody (Pembrolizumab, #A2005) and anti-CTLA-4 antibody (#A2001) was purchased from Selleck Chemicals (Houston, TX, USA).
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3

Antioxidant Activity of Plant Extracts

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The standard substances α-pinene (98%), sabinene (95%), p-cymene (99%), limonene (97%), linalool (97%), γ-terpinene (96%), (E)-β-caryophyllene (98.5%), germacrene D (90%), α-humulene (96%), caryophyllene oxide (95%) (98%), p-hydroxybenzoic acid (99%), caffeic acid (98%), o-hydroxybenzoic acid (99%), rosmarinic acid (97%), rutin (94%), AAPH (97%), and fluorescein (99%) were purchased from Sigma Aldrich (St. Louise, MO, USA), and luteolin-7-O-glucoside (98%), kaempferol-3-O-rutinoside (98%), and salvigenin (98%) were purchased from ChemFaces (Wuhan, China); vitexin (≥95%) was purchased from PhytoLab GmbH (Vestenbergsgreuth, Bavaria, Germany). Solvents and other reagents used in the extraction process, GC/FID grade dichloromethane, ammonium formate (99%), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), formic acid HPLC grade, potassium persulfate (99%), and absolute ethanol (96%) were purchased from Merck (Darmstadt, Germany); commercial ethanol (96%) was acquired from SUQUIN (Bucaramanga, Santander, Colombia).
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4

Antioxidant and Chemical Profiling

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Trolox®, α-tocopherol, butylated hydroxytoluene (BHT), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), fluorescein, sodium persulfate, sodium acetate, n-tetradecane (≥99%), quercetin-3-O-glucoside (≥90%), rosmarinic acid (≥97%), naringenin (≥95%), apigenin (≥95%), eupatilin (≥98%), ursolic acid (≥90%), rutin (≥94%), (E)-β-caryophyllene (≥98.5%), α-humulene (≥96%), (2E, 6Z)-farnesol (≥96%), benzyl benzoate (≥99%), and benzyl salicylate (≥99%) were acquired from Sigma-Aldrich (St. Louis, MO, USA). Dipotassium phosphate was obtained from J.T. Baker (Phillipsburg, NJ, USA). Luteolin-7-O-glucoside (≥98%), luteolin-7-O-glucuronide (≥98%), cirsimaritin (≥98%), acacetin (≥98%), scopoletin (≥98%), and salvigenin (≥98%) were acquired from ChemFaces (Wuhan, China). Vitexin (≥95%) was obtained from Phytolab (Vestenbergsgreuth, Germany). Luteolin (≥98%) was purchased from Chemcruz (Santa Cruz Biotech., Dallas, TX, USA). The mixture of n-alkanes C8-C25 was obtained from AccuStandard, Inc. (New Haven, CT, USA). Helium, air, hydrogen, and nitrogen (99.995%) for GC analysis were purchased from Messer (Bucaramanga, Colombia). Ethanol (96%) was acquired from Suquin S.A.S. (Bucaramanga, Colombia). Type I water (18.2 MΩ cm) was produced using a Millipore Direct-QTM (Merck, Darmstadt, Germany) purification system.
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