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Rabbit anti f4 80 antibodies

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Rabbit anti-F4/80 antibodies are a laboratory product used to detect the F4/80 antigen, which is commonly expressed on the surface of macrophages. These antibodies can be used in various immunological techniques, such as flow cytometry, immunohistochemistry, and Western blotting, to identify and study macrophages in biological samples.

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Lab products found in correlation

Tcs spe confocal microscope system, by Leica (2 mentions) 7f6 g5 a2, by Santa Cruz Biotechnology (2 mentions) Mca497, by Bio-Rad (1 mentions)

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F4/80 (201 citations) Anti-F4/80 (70 citations)

8 protocols using rabbit anti f4 80 antibodies

1

Histological Analysis of Mouse Liver

Cited 2 times
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Paraffin-embedded mouse liver blocks were cut into sections of 5 μm thickness and stained with hematoxylin and eosin (H&E) and/or stained for F4/80 expression with rabbit anti-F4/80 antibodies (1:100) (AbD Serotec, Raleigh, NC, USA).6 (link),23 (link) The fraction of F4/80-expressing cells for each sample is calculated as the sum of the number of nuclei of F4/80-expressing cells divided by the total number of nuclei in sections of a sample. Six fields per slide were included, and a total of 4–6 mice per group were used. Frozen liver sections (4–5 μm thick) were stained with Oil Red O as described.6 (link)
Guo X., Zhu B., Xu H., Li H., Jiang B., Wang Y., Zheng B., Glaser S., Alpini G, & Wu C. (2020). Adoptive transfer of Pfkfb3-disrupted hematopoietic cells to wild-type mice exacerbates diet-induced hepatic steatosis and inflammation. Liver research, 4(3), 136-144.
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2

Histological Analysis of Mouse Liver

Cited 2 times
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Paraffin-embedded mouse liver blocks were cut into sections of 5 μm thickness and stained with hematoxylin and eosin (H&E) and/or stained for F4/80 expression with rabbit anti-F4/80 antibodies (1:100) (AbD Serotec, Raleigh, NC, USA).6 (link),23 (link) The fraction of F4/80-expressing cells for each sample is calculated as the sum of the number of nuclei of F4/80-expressing cells divided by the total number of nuclei in sections of a sample. Six fields per slide were included, and a total of 4–6 mice per group were used. Frozen liver sections (4–5 μm thick) were stained with Oil Red O as described.6 (link)
Guo X., Zhu B., Xu H., Li H., Jiang B., Wang Y., Zheng B., Glaser S., Alpini G, & Wu C. (2020). Adoptive transfer of Pfkfb3-disrupted hematopoietic cells to wild-type mice exacerbates diet-induced hepatic steatosis and inflammation. Liver research, 4(3), 136-144.
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3

Histological Tissue Analysis via H&E and F4/80

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The paraffin-embedded tissue blocks were cut into sections of 5 µm thickness and stained with H&E and/or stained for the expression of F4/80 with rabbit anti-F4/80 antibodies (1:100) (AbD Serotec, Raleigh, NC).
Guo X., Shu C., Li H., Pei Y., Woo S.L., Zheng J., Liu M., Xu H., Botchlett R., Guo T., Cai Y., Gao X., Zhou J., Chen L., Li Q., Xiao X., Xie L., Zhang K.K., Ji J.Y., Huo Y., Meng F., Alpini G., Li P, & Wu C. (2017). Cyclic GMP-AMP Ameliorates Diet-induced Metabolic Dysregulation and Regulates Proinflammatory Responses Distinctly from STING Activation. Scientific Reports, 7, 6355.
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4

Immunohistochemical Analysis of A2A Receptor and Macrophage in Adipose Tissue

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Paraffin-embedded adipose tissue (epididymal fat) blocks were cut into sections of 5 µm thickness. The sections of adipose tissue from Study 1 were stained for A2AR expression using mouse monoclonal antibodies against A2AR (7F6-G5-A2, Cat# sc-32261, Santa Cruz Biotechnology, Inc., Dallas, TX, USA). Additional sections of adipose tissue from study 1, along with those from Study 2, were stained with H&E and for F4/80 expression with rabbit anti-F4/80 antibodies (1:100) (AbD Serotec, Raleigh, NC). Also, the co-staining of F4/80 (rat anti-mouse, MCA497, Bio-Rad, Hercules, CA) and A2AR in adipose tissue sections was evaluated by double immunofluorescent labeling according to the manufacturer’s instructions (Vector Laboratories, Inc. Burlingame, CA). Following staining, images were obtained using Leica TCS SPE Confocal Microscope System (Buffalo Grove, IL).
Pei Y., Li H., Cai Y., Zhou J., Luo X., Ma L., McDaniel K., Zeng T., Chen Y., Qian X., Huo Y., Glaser S., Meng F., Alpini G., Chen L, & Wu C. (2018). Regulation of Adipose Tissue Inflammation by Adenosine 2A Receptor in Obese Mice. The Journal of endocrinology, 239(3), 365-376.
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5

Adipose Tissue Histological Analysis

Cited 1 time
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Paraffin-embedded mouse WAT blocks were cut into sections of 5 µm thickness and stained with hematoxylin and eosin (H&E) and/or stained for F4/80 expression with rabbit anti-F4/80 antibodies (1:100) (AbD Serotec, Raleigh, NC) 4 (link), 6 .
Zhu B., Guo X., Xu H., Jiang B., Li H., Wang Y., Yin Q., Zhou T., Cai J.J., Glaser S., Meng F., Francis H., Alpini G, & Wu C. (2021). Adipose Tissue Inflammation and Systemic Insulin Resistance in Mice with Diet-induced Obesity Is Possibly Associated with Disruption of PFKFB3 in Hematopoietic Cells. Laboratory investigation; a journal of technical methods and pathology, 101(3), 328-340.
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6

Adipose Tissue Histological Analysis

Cited 1 time
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Paraffin-embedded mouse WAT blocks were cut into sections of 5 µm thickness and stained with hematoxylin and eosin (H&E) and/or stained for F4/80 expression with rabbit anti-F4/80 antibodies (1:100) (AbD Serotec, Raleigh, NC) 4 (link), 6 .
Zhu B., Guo X., Xu H., Jiang B., Li H., Wang Y., Yin Q., Zhou T., Cai J.J., Glaser S., Meng F., Francis H., Alpini G, & Wu C. (2021). Adipose Tissue Inflammation and Systemic Insulin Resistance in Mice with Diet-induced Obesity Is Possibly Associated with Disruption of PFKFB3 in Hematopoietic Cells. Laboratory investigation; a journal of technical methods and pathology, 101(3), 328-340.
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7

Histological Analysis of Mouse Adipose Tissue

Cited 1 time
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Paraffin-embedded mouse WAT blocks were cut into sections of 5 μm thickness and stained with hematoxylin and eosin (H&E) and/or stained for F4/80 expression with rabbit anti-F4/80 antibodies (1:1,000) (AbD Serotec, Raleigh, NC) [29 ,31 (link)].
Xu H., Zhu B., Li H., Jiang B., Wang Y., Yin Q., Cai J., Glaser S., Francis H., Alpini G, & Wu C. (2021). Adipocyte inducible 6-phosphofructo-2-kinase suppresses adipose tissue inflammation and promotes macrophage anti-inflammatory activation. The Journal of nutritional biochemistry, 95, 108764.
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8

Immunohistochemical Analysis of Liver Inflammation

Cited 1 time
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The sections of paraffin-embedded liver blocks were stained with H&E and/or stained for F4/80 expression with rabbit anti-F4/80 antibodies (1:100) (AbD Serotec, Raleigh, NC). Also, co-staining of F4/80 (rat anti-mouse, MCA497, Bio-Rad, Hercules, CA) and A2AR (7F6-G5-A2, Cat# sc-32261, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in liver sections was evaluated by double immunofluorescent labeling according to the manufacturer’s instructions (Vector Laboratories, Inc. Burlingame, CA) as previously described (Pei, et al. 2018 ). Following staining, images were obtained using Leica TCS SPE Confocal Microscope System (Buffalo Grove, IL). The sections of frozen livers were stained with Oil-Red-O as previously described (Guo et al. 2016 (link)).
Zhou J., Li H., Cai Y., Ma L., Matthews D., Lu B., Zhu B., Chen Y., Qian X., Xiao X., Li Q., Guo S., Huo Y., Zhao L., Tian Y., Li Q, & Wu C. (2019). Mice Lacking Adenosine 2A Receptor Reveal Increased Severity of MCD-induced NASH. The Journal of endocrinology.
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