Sorafenib tosylate

Manufactured by Biosynth

Sourced in United Kingdom

Sorafenib Tosylate is a pharmaceutical compound used in research and development. It is a tyrosine kinase inhibitor that targets multiple kinases involved in tumor cell proliferation and angiogenesis. Sorafenib Tosylate is commonly used in cell-based and biochemical assays to investigate its effects on signal transduction pathways and cellular processes.

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Lab products found in correlation

4 amino 5 methylamino 2 7 difluorofluorescein daf fm diacetate, by Thermo Fisher Scientific (1 mentions) Triethylamine, by Merck Group (1 mentions) Cremophor el, by Merck Group (1 mentions) Lauroglycol 90, by Gattefosse (1 mentions) Labrafac lipophile wl 1349, by Gattefosse (1 mentions) Capryol 90, by Gattefosse (1 mentions) Peceol, by Gattefosse (1 mentions) Tetraglycol, by Merck Group (1 mentions) Polyvinylpyrrolidone pvp k30, by Merck Group (1 mentions) Labrafil m 2125, by Gattefosse (1 mentions) Glycerin, by Daejung Chemicals (1 mentions) Transcutol p, by Gattefosse (1 mentions) Labrasol, by Gattefosse (1 mentions) Span 80, by Daejung Chemicals (1 mentions) Tween 80, by Daejung Chemicals (1 mentions) Span 20, by Daejung Chemicals (1 mentions) Aerosil 200 pharma, by Evonik (1 mentions) Soybean oil, by Daejung Chemicals (1 mentions) Corn oil, by Daejung Chemicals (1 mentions) Polyethylene glycol peg 400, by Samchun (1 mentions)

3 protocols using sorafenib tosylate

Quantification of Nitric Oxide Levels

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All reagents were from Sigma-Aldrich Chemical Co. (St Louis, Missouri, USA) unless otherwise stated. 4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM) was obtained from Molecular Probes (Cat. D-23842). S-nitroso-l-cysteine (CSNO) was prepared according to the procedure described elsewhere [31] (link) by incubation of l-cysteine with acidified sodium nitrite and quantification by absorbance at 334 nm using a molar absorption coefficient of 0.74 mM⁻¹ cm⁻¹. Sorafenib Tosylate was purchased from Carbosynth (Carbosynth Ltd., Berkshire, United Kingdom).The study protocol has been approved by the Ethical Committee of the Institution.

Rodríguez-Hernández A., Navarro-Villarán E., González R., Pereira S., Soriano-De Castro L.B., Sarrias-Giménez A., Barrera-Pulido L., Álamo-Martínez J.M., Serrablo-Requejo A., Blanco-Fernández G., Nogales-Muñoz A., Gila-Bohórquez A., Pacheco D., Torres-Nieto M.A., Serrano-Díaz-Canedo J., Suárez-Artacho G., Bernal-Bellido C., Marín-Gómez L.M., Barcena J.A., Gómez-Bravo M.A., Padilla C.A., Padillo F.J, & Muntané J. (2015). Regulation of cell death receptor S-nitrosylation and apoptotic signaling by Sorafenib in hepatoblastoma cells. Redox Biology, 6, 174-182.

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Sorafenib Tosylate Formulation Development

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Sorafenib tosylate was purchased from BIOSYNTH Carbosynth (Compton, Newbury, United Kingdom). Cremophor EL, tetraglycol, triethylamine, and polyvinylpyrrolidone (PVP K 30) (average molecular weight 40 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Triacetin, isopropyl myristate (IPM), soybean oil, corn oil, tween 80, span 80, span 20, mineral oil, and glycerin were acquired from Daejung Chemicals Co. (Siheung, South Korea). Peceol, labrafil M 1994 CS, labrafil M 2125, labrasol, transcutol P, lauroglycol 90, capryol 90, and labrafac lipophile WL 1349 were obtained from Gattefossé (Saint-Priest Cedex, France). Capmul MCM and captex 355 were gifted from Abitec Corporation (Columbus, OH, USA). Propylene glycol, tween 20, olive oil, and phosphoric acid were purchased from Duksan Pure Chemical (Ansan, South Korea). Polyethylene glycol (PEG) 400 was purchased from Samchun Pure Chemical (Gyeonggi-Do, South Korea). Colloidal silica (Aerosil^® 200 Pharma) was purchased from Evonik industries AG (Essen, Germany). Ethanol (EtOH) and high-performance liquid chromatography (HPLC)-grade acetonitrile (ACN) were purchased from Honeywell Burdick & Jackson^® (Muskegon, MI, USA). All other chemicals used were of the analytical grade.

Fakhrai H., Dorigo O., Shawler D.L., Lin H., Mercola D., Black K.L., Royston I, & Sobol R.E. (1996). Eradication of established intracranial rat gliomas by transforming growth factor beta antisense gene therapy.. Proceedings of the National Academy of Sciences of the United States of America, 93(7), 2909-2914.

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Sorafenib Tosylate Oxidative Stress Assay

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Sorafenib Tosylate was obtained from Carbosynth Limited (Berkshire, UK) (Supplementary Fig. 1). Xanthine oxidase (X2252), xanthine (X0626), 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) and cyclic hydroxylamine 1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine (CMH) were obtained from Sigma-Aldrich (Missouri, USA). Red Mitochondrial Superoxide Indicator (MitoSOX™) (M36008), dihydroethidium (DHE) (D-11347), 2,7-dichloro dihydro-fluorescein diacetat (H₂DCFDA) (D-399) and 4,5-diaminofluorescein diacetate (DAF-2) (D-23842) were obtained from Life Technologies (Thermo Fisher Scientific, Massachusetts, USA). Sulforaphane (SFN, Sigma-Aldrich) and vascular endothelial growth factor (VEGF, PrepoTech, New Jersey, USA) were used as inducers of Nrf2 activation.
HepG2 (HB-8065™) was obtained from ATCC/LGC Standards (Barcelona, Spain). JHH2, JHH-4, JHH-5 and Huh-1 were obtained from the Japanese Collection of Research Bioresources Cell Bank (Tokyo, Japan). SNU886 was obtained from the Korean Cell Lines Bank (Seoul, Korea). MHCC97H was obtained from Woodland Pharmaceuticals (Massachusetts, USA).

González R., Rodríguez-Hernández M.A., Negrete M., Ranguelova K., Rossin A., Choya-Foces C., Cruz-Ojeda P.D., Miranda-Vizuete A., Martínez-Ruiz A., Rius-Pérez S., Sastre J., Bárcena J.A., Hueber A.O., Padilla C.A, & Muntané J. (2020). Downregulation of thioredoxin-1-dependent CD95 S-nitrosation by Sorafenib reduces liver cancer. Redox Biology, 34, 101528.

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