Formaldehyde was purchased from Sinopharm Chemical Reagent Co., Beijing. Ultrapure Tris was purchased from the USB Corporation (Affymetrix, Santa Clara, California, USA). All of the antibodies used were purchased from commercial sources. These antibodies included a mouse monoclonal anti-human serum albumin antibody for immunocytochemistry applications (ab10241, Abcam, Boston, MA, USA) and a peroxidase-conjugated AffiniPure goat anti-mouse IgG (H + L) (ZB-2305, ZSGB-bio, Beijing, China). Protein A + G agarose for co-immunoprecipitation was purchased from the Beyotime Institute of Biotechnology (P2012, Shanghai, China). PBS for washing was obtained from the Cell Culture Center at Peking Union Medical College (Beijing, China).
For protein electrophoresis, 1.5 mm × 10 well 4-12% Bis-Tris gels (NuPAGE, Life Technologies, Carlsbad, California, USA) were used. The 5× SDS loading buffer was obtained from Genestar (Shanghai, China), and the 1851 protein marker was obtained from Fermentas (Waltham, Massachusetts, USA). For trypsin digestion, trypsin gold of mass spectrometry grade was purchased from Promega (Madison, WI, USA). IAA (iodoacetamide) was purchased from GE (Fairfield, Connecticut, USA), and DTT (dithiothreitol) was purchased from Merck (Whitehouse Station, New Jersey, USA). All other regents were of high purity.
For protein electrophoresis, 1.5 mm × 10 well 4-12% Bis-Tris gels (NuPAGE, Life Technologies, Carlsbad, California, USA) were used. The 5× SDS loading buffer was obtained from Genestar (Shanghai, China), and the 1851 protein marker was obtained from Fermentas (Waltham, Massachusetts, USA). For trypsin digestion, trypsin gold of mass spectrometry grade was purchased from Promega (Madison, WI, USA). IAA (iodoacetamide) was purchased from GE (Fairfield, Connecticut, USA), and DTT (dithiothreitol) was purchased from Merck (Whitehouse Station, New Jersey, USA). All other regents were of high purity.