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Inverted system microscope

Manufactured by Olympus
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The Inverted system microscope is a versatile laboratory instrument designed for the observation and analysis of specimens. It features an inverted optical configuration, with the light source and objectives positioned below the stage, allowing for the examination of thick or opaque samples. The microscope's core function is to provide high-resolution, detailed images of the specimen under investigation.

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Lab products found in correlation

Methocult gf m3434 medium, by STEMCELL (1 mentions) Methocult gf h4435, by STEMCELL (1 mentions) Dp71 camera, by Olympus (1 mentions) Cell d software, by Olympus (1 mentions)

5 protocols using inverted system microscope

1

Thoracic Aorta Collagen Quantification

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The thoracic aortae were dissected and processed as described above. Collagen staining was performed using Masson’s trichrome staining as described [16 (link)]. The stained sections were viewed in a blinded manner with an Olympus® inverted system microscope as described above. Percentage collagen positive area was analyzed using ImageJ 1.42.
Pingili A.K., Jennings B.L., Mukherjee K., Akroush W., Gonzalez F.J, & Malik K.U. (2020). 6β-Hydroxytestosterone, a metabolite of testosterone generated by CYP1B1, contributes to vascular changes in angiotensin II-induced hypertension in male mice. Biology of Sex Differences, 11, 4.
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2

Cryosectioning and Histological Staining of Vessels

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Following anesthesia (described above), the vessels were dissected free, cleaned of surrounding tissue, placed in O.C.T. compound, and frozen at −80 °C. 10 μm sections were cut using a cryostat and stained with standard hematoxylin and eosin. Sections were viewed with an Olympus® inverted system microscope (Olympus America, Inc., Melville, NY; model BX41), photographed using a SPOT™ Insight™ digital camera (Diagnostic Instruments, Inc., Sterling Heights, MI; model Insight 2MP Firewire), and images were analyzed using ImageJ 1.42.
Jennings B.L., Montanez D.E., May ME J.r., Estes A.M., Fang X.R., Yaghini F.A., Malik K.U, & Kanu A. (2014). Cytochrome P450 1B1 contributes to increased blood pressure and cardiovascular and renal dysfunction in spontaneously hypertensive rats. Cardiovascular drugs and therapy / sponsored by the International Society of Cardiovascular Pharmacotherapy, 28(2), 145-161.
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3

Peripheral Blood and Bone Marrow Analysis

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Peripheral blood was collected from mice by retro-orbital bleeding and complete blood count was performed by an automated blood count (Hemavet System 950FS). Bone marrow cytopspin was performed after euthanized the mice. May-Grunwald–Giemsa staining was used for morphologic analysis of bone marrow cytospin. All images were taken using an inverted system microscope (Olympus).
Man N., Mas G., Karl D.L., Sun J., Liu F., Yang Q., Torres-Martin M., Itonaga H., Martinez C., Chen S., Xu Y., Duffort S., Hamard P.J., Chen C., Zucconi B.E., Cimmino L., Yang F.C., Xu M., Cole P.A., Figueroa M.E, & Nimer S.D. (2021). p300 suppresses the transition of myelodysplastic syndromes to acute myeloid leukemia. JCI Insight, 6(19), e138478.
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4

Colony Assay of Leukemia Cells

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For colony assay of AML cells (Kasumi-1, THP-1, AE9a and MLL-AF9 cells), cells were infected with lentivirus expressing shRNA against UHRF1 for 48 h (scrambled shRNA as control) using PLKO.1 vector. After AML cells were selected with puromycin for 48 h, they were cultured in Methocult GF-H4435 or Methocult GF-M3434 medium (Stem Cell Technologies). Uhrf1fl/fl Cre-ER/Uhrf1fl/fl fetal liver cells were isolated from E14.5 embryos, and Uhrf1fl/fl Cre-ER/Uhrf1fl/fl BM cells were isolated from 6‒8 weeks old mice. These cells were cultured in the RMPI 1640 medium with 10% fetal bovine serum supplemented with 100 ng/mL SCF, 10 ng/mL IL-3 and 10 ng/mL IL-6, and were infected with MIGR1-based retrovirus expressing AML1-ETO-9a (AE9a) or MLL-AF9 (MA9). GFP+LSK (fetal liver) or GFP+L-GMP (BM) cells were sorted 48 h after the infection. These LICs were cultured in Methocult GF-M3434 medium (Stem Cell Technologies) supplemented with 1 µM 4-OH-Tamoxifen. The colonic cells were replated weekly. All colony number was counted on day 7 using an inverted system microscope (Olympus).
Hu C.L., Chen B.Y., Li Z., Yang T., Xu C.H., Yang R., Yu P.C., Zhao J., Liu T., Liu N., Shan B., Zhang Q., Song J., Fei M.Y., Zong L.J., Zhang J.Y., Wu J.C., Chen S.B., Wang Y., Chang B., Hou D., Liu P., Jiang Y., Li X., Chen X., Deng C.H., Ren Y.Y., Wang R., Jin J., Xue K., Zhang Y., Du M., Shi J., Wu L.Y., Chang C.K., Shen S., Chen Z., Chen S.J., Liu X., Sun X.J., Zheng M, & Wang L. (2022). Targeting UHRF1-SAP30-MXD4 axis for leukemia initiating cell eradication in myeloid leukemia. Cell Research, 32(12), 1105-1123.
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5

Anorectal Histological Analysis in Rats

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On the 14th day, 1 h after the treatment, rat anorectal histology samples were obtained by fixing rat anorectal biopsies into 10% formalin solution, paraffin embedding, dissection and hematoxylin-eosin staining. All sample histology slides were observed using an Inverted system microscope, IX71-IX2 series optical microscope, the DP71 camera, and Cell D software (Olympus; Shinjuku-ku, Tokyo, Japan). Histological parameters such as the number of inflammatory cells, congestion, bleeding, vasodilation, and necrosis are observed in the histological preparations of anorectal tissue (Nishiki et al., 1988; Azeemuddin et al., 2014) .
Kusumawati I., Rullyansyah S., R.o.h.m.a.n.i.a., Rizka A.F., Hestianah E.P, & Matsunami K. (2022). Histomorphometric study of ethanolic extract of Graptophyllum pictum (L.) Griff. leaves on croton oil-induced hemorrhoid mice: A Javanese traditional anti-hemorrhoid herb. Journal of ethnopharmacology, 284.
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