Goat anti mouse and goat anti rabbit hrp conjugated antibodies

Manufactured by Cell Signaling Technology

Sourced in Romania

Goat anti-mouse and goat anti-rabbit HRP conjugated antibodies are secondary antibodies used in immunoassays and immunoblotting applications. They are designed to bind to primary antibodies raised in mouse or rabbit and are conjugated with horseradish peroxidase (HRP) enzyme for signal detection.

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Lab products found in correlation

Odyssey system, by LI COR (4 mentions) Cgp57380, by Selleck Chemicals (1 mentions) Gapdh, by Merck Group (1 mentions) C myc, by Abcam (1 mentions) Survivin, by Abcam (1 mentions) Ab3259, by Abcam (1 mentions) Ab184699, by Abcam (1 mentions) N wasp, by Merck Group (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions)

4 protocols using goat anti mouse and goat anti rabbit hrp conjugated antibodies

Western Blot Analysis of Signaling Proteins

Cited 2 times

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The procedure used for western blot analysis was as previously described (18 (link),23 (link)). Polyclonal rabbit antibodies against MKNK2 (Cat. no. ab84345), eIF4E (Cat. no. ab33766), phospho-eIF4E (Cat. no. ab76256), cMYC (Cat. no. ab39688) and FLI1 (Cat. no. ab133485) were all purchased from Abcam; MKNK1 (Cat. no. 2195) and survivin (Cat. no. 2808) antibodies were obtained from Cell Signaling Technology); GAPDH (Cat. no. G9545) antibody was obtained from Sigma-Aldrich; β-actin antibody (Cat. no. 20536-1-AP) was obtained from Proto-Technology (Protein-Tech); goat-anti-mouse and goat anti-rabbit HRP-conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos. 5470s and 5151s, respectively). Primary antibodies were added to the filters and incubated overnight at 4̊C. After washing, secondary antibodies were added for 1 h at room temperature. Antibody dilution was according to the manufacturer’s instructions. The Odyssey system (LI-COR Biosciences) was used to image proteins in western blot analysis.
The inhibitor of MKNK1 (CGP57380) was obtained from Selleckchem. The Fli-1 inhibitors, A1544 and A1545, were used as previously described (22 (link)).

Wang C., Song J., Liu W., Yao Y., Kapranov P., Sample K.M., Gajendran B., Zacksenhaus E., Hao X, & Ben-David Y. (2019). FLI1 promotes protein translation via the transcriptional regulation of MKNK1 expression. International Journal of Oncology, 56(2), 430-438.

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Western Blot Quantification of ERK, SREBP1, and EGR1

Cited 1 time

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Previously published standard protocols were used to perform the western blot experiments [22 (link)]. Using the following antibodies: Polyclonal rabbit antibodies for ERK (ab184699) and SREBP1 (ab3259) was purchased from Abcam; the EGR1 (Cat. no.22008–1-AP) antibody was obtained from Proto Technology (Proteintech, Bucuresti, Romania); the phospho ERK (Cat. no.9101S) antibody was obtained from Cell Signaling Technology (CST, Danvers, MA01923); the GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. no. 5470 s and 5151 s). Antibody dilution was conducted according to the manufacturer’s instructions. The Odyssey system (LI COR Biosciences) was used to analyse the protein detection.

Yu F., Gajendran B., Wang N., Sample K.M., Liu W., Wang C., Hu A., Zacksenhaus E., Hao X, & Ben-David Y. (2021). ERK activation via A1542/3 limonoids attenuates erythroleukemia through transcriptional stimulation of cholesterol biosynthesis genes. BMC Cancer, 21, 680.

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Western Blotting: Antibody Optimization

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Western blotting was performed, as previously described (22 (link), 23 (link)). The following antibodies were used: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485), ERK (ab184699) and FLI1ChIP grade (Cat. no. ab15289) were all purchased from Abcam; the WIP (Cat. no. PA5-51995) antibody was obtained from Invitrogen (Invitrogen; the phospho-ERK (Cat. no.9101S) antibody was obtained from Cell Signaling Technology (CST, Danvers, MA01923); the GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; WASP (Cat. no. 10879-1-AP), N-WASP (Cat. no. 14306-1-AP), GATA1 (Cat. no. 10917-2-AP), β-actin antibodies (Cat. no. 20536 1 AP) were obtained from Proto Technology (Proteintech, Bucuresti, Romania); goat anti-mouse and goat anti-rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos. 5470s and 5151s, respectively). Antibody dilution was conducted according to the manufacturer’s instructions. The Odyssey system (LI COR Biosciences) and Bio were used to image proteins in western blot analysis.
The inhibitor of N-WASP (Wiskostatin) was obtained from Cayman Chemica (Cat. no. 15047-10). The development of our specific FLI1 inhibitory compounds A661 and A665 has previously been described (22 (link)).

Wang C., Sample K.M., Gajendran B., Kapranov P., Liu W., Hu A., Zacksenhaus E., Li Y., Hao X, & Ben-David Y. (2021). FLI1 Induces Megakaryopoiesis Gene Expression Through WAS/WIP-Dependent and Independent Mechanisms; Implications for Wiskott-Aldrich Syndrome. Frontiers in Immunology, 12, 607836.

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Western Blotting of FLI1 and HDC

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Western blotting was done using protocol, as previously described.19 (link),21 (link) The antibodies used are as follows: Polyclonal rabbit antibodies for FLI1 (Cat. no. ab133485) and HDC (Cat. no. ab137571) were obtained from Abcam, the monoclonal GAPDH (Cat. no. G9545) antibody was obtained from Sigma Aldrich; goat anti mouse and goat anti rabbit HRP conjugated antibodies were obtained from Cell Signaling Technology (Cat. nos. 5470s and 5151s, respectively). Antibody dilution was conducted according to the manufacturer’s instructions. The Odyssey system (LI COR Biosciences) and Bio was used to image proteins in Western blot analysis.

Hu J., Gao J., Wang C., Liu W., Hu A., Xiao X., Kuang Y., Yu K., Gajendran B., Zacksenhaus E., Pan W, & Ben-David Y. (2023). FLI1 Regulates Histamine Decarboxylase Expression to Control Inflammation Signaling and Leukemia Progression. Journal of Inflammation Research, 16, 2007-2020.

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