Chondrogenesis differentiation medium
Chondrogenesis differentiation medium is a culture medium designed to support the differentiation of mesenchymal stem cells (MSCs) into chondrocytes, the cells responsible for the formation of cartilage. The medium provides the necessary components to promote the chondrogenic lineage commitment and maturation of MSCs.
Lab products found in correlation
5 protocols using chondrogenesis differentiation medium
Chondrogenic Differentiation in 3D Scaffolds
Quantitative Analysis of Stem Cell Differentiation
For the chondrogenesis assay, pellets were prepared by spinning down 1 × 105 cells and incubating in a 15-mL conical tube in chondrogenesis differentiation medium (Invitrogen) with the medium changed every 3 days. After 28 days of culturing, cells were fixed with 4% formaldehyde, and stained with Alcian Blue (Merck, Darmstadt, Germany).
Chondrogenesis Differentiation of rMDSC Pellets
Chondrocyte Differentiation Assay
To demonstrate positive effect of differentiation into chondrocytes cells were stained with 1% Alcian Blue solution (Sigma-Aldrich). Fixed WJ-MSC or AD-MSC were stained with prepared solution for 30 minutes and washed three times with 0.1 N HCl. At the end, to neutralize acidity the cells were rinsed with distilled water.
Chondrogenic Pellet Culture Protocol
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!