We tested PCR compatibility by incubating the head overnight in 3 mL of modified CDC VTM (Hank’s balanced salt solution containing: 2% heat inactivated FBS, 100μg/mL gentamicin, 0.5μg/mL fungizone, and 10mg/L Phenol red (11 )) to allow any PCR-inhibitory material to leach into the medium, spiking 1.5mL with 200 copies/mL of control SARS-CoV-2 amplicon target (representing 2 times the limit of detection on our system), vortexing, and testing using the Abbott RealTime SARS-CoV-2 Assay on an Abbott m2000 RealTime System platform (12 ), following the same protocol as for clinical testing. PCR-positive prototypes passed.
M2000 realtime system platform
Manufactured by Abbott
The M2000 RealTime System platform is a fully automated system for real-time PCR analysis. The system performs nucleic acid extraction, amplification, and detection in a single, integrated process. It is designed to provide accurate and reliable results for a variety of diagnostic and research applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using m2000 realtime system platform
1
Evaluating PCR Compatibility of Samples
2
PCR Compatibility Assessment of Swab Types
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Although all swabs are routinely used for PCR testing, as a double-check each swab type was assessed for PCR compatibility by overnight incubation in 3 mL of modified CDC VTM (allowing potential PCR inhibitors time to leech into media), spiking 1.5 mL of media with 200 copies/mL of control SARS-CoV-2 amplicon target (twice the LoD of our system), vortexed, and tested using the Abbott RealTime SARS-CoV-2 Assay on an Abbott m2000 RealTime System platform18 , the assay and platform used for all testing in this report, following the same protocol used for clinical testing (see below). All swabs examined in this study passed this quality-control testing for lack of RT-PCR inhibition based on observation of Ct values within expected quality control limits17 (link).
3
Assessing PCR Compatibility of Swab Types
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Although all of the above swabs are routinely used for PCR testing, as a double-check, each swab type was assessed for PCR compatibility by overnight incubation in 3 ml of modified CDC VTM (allowing potential PCR inhibitors time to leech into media), spiking 1.5 ml of medium with 200 copies/ml of control SARS-CoV-2 amplicon target (twice the LoD of our system), vortexing, and testing using the Abbott RealTime SARS-CoV-2 assay on an Abbott m2000 RealTime system platform (18 ), the assay and platform used for all testing in this report, following the same protocol used for clinical testing (see below). All swabs examined in this study passed this quality-control testing for lack of RT-PCR inhibition based on observation of cycle threshold (CT) values within expected quality control limits (17 (link)).
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