Anhydrous ammonium acetate

Metformin, D-glucose, L-alanine, bovine serum albumin (BSA, fraction V), collagenase (from Clostridium histolyticum), epinephrine, insulin, dibutyryl-cAMP (DB-cAMP), 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), heptane, silicone oil, phloretin, and all reagents used to prepare Krebs-Ringer buffer (118 mM NaCl, 4.8 mM KCl, 1.3 mM, CaCl₂, 1.2 mM KH₂PO₄, 1.2 mM MgSO₄, 24.8 mM, NaHCO₃) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Reagents for glycerol assay: isopropanol, anhydrous ammonium acetate, sodium metaperiodate, and 2,4-pentadione were purchased from Sigma-Aldrich (St. Louis, MO, USA), and glacial acetic acid was obtained from POCH (Gliwice, Poland).
Reagents used for the determination of non-esterified fatty acids (NEFAs): triethanolamine, acetic acid, and diethyldithiocarbamate were also purchased from Sigma-Aldrich (St. Louis, MO, USA), whereas butanol-1 and chloroform were obtained from POCH (Gliwice, Poland).
Lactate was measured using lactate dehydrogenase, NAD⁺, and glycine buffer (Sigma-Aldrich, St. Louis, MO, USA). Glucose D-[¹⁴C(U)], 2-deoxy-D-glucose-[1-¹⁴C], and scintillation cocktail-OptiPhase HiSafe were purchased from Perkin Elmer (Boston, MA, USA).

Acetonitrile, glacial acetic acid, and formic acid (all HPLC grade) were obtained from Sigma-Aldrich (Saint Louis, MO, US). Polymerically bonded ethylenediamine-N-propyl phase (PSA) was purchased from Varian (Palo Alto, CA, USA), while anhydrous magnesium sulfate, sodium chloride, anhydrous ammonium acetate, and C18 were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Aqueous solutions were prepared with ultrapure-water (Milli-Q Plus system; Millipore Corp., Billerica, MA, USA). Prochloraz and its metabolites (2,4,6-trichlorophenol (2,4,6-TCP), 1-propyl-1-[2-(2,4,6 trichlorophenoxy)ethyl]urea (BTS 44595), 3-formyl-1-propyl-1-[2-(2,4,6-trichlorophenoxy)ethyl]urea (BTS 44596)], and fludioxonil were of high purity grade (>99.0%), purchased from Sigma-Aldrich (Saint Louis, MO, USA). Individual stock solutions were prepared at 1000 mg/L in Acetonitrile and stored at −20 °C. The working solutions were prepared by carrying out appropriate dilutions of the stock solutions.

Acetonitrile, glacial acetic acid, methanol, ethyl acetate and formic acid (all of HPLC grade) were obtained from Sigma-Aldrich (Saint Louis, MO, US). Polymerically bonded ethylenediamine-N-propyl phase (PSA) was purchased from Varian (Palo Alto, CA, USA), while anhydrous magnesium sulfate, florisil, anhydrous ammonium acetate and sodium acetate were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Aqueous solutions were prepared with ultra-pure-water (Milli-Q Plus system; Millipore Corp., Billerica, MA, USA). All pesticide standards were of high purity grade (>99.0%), purchased from Sigma-Aldrich (Saint Louis, MO, USA). Individual stock solutions were prepared at 1000 mg/L in Acetonitrile or methanol and stored at -20°C. The working solutions were prepared by carrying out appropriate dilutions of the stock solutions.