Cocl2.2h2o

The chemicals BaCl₂.2H₂O, CoCl₂.2H₂O, and CuCl₂.6H₂O (reagent grade RG) were purchased from Merck, FeCl₃ was bought from Sigma Aldrich, >98% purity citric acid and C₆H₈O₇ from Merck, RG grade propylene glycol (C₃H₈O₂) and glycerin (C₃H₈O₃) were from Merck. NH₄OH (RG grade) and acetaminophen (Analytical grade AG) were used as received from Merck. The AG-grade PBS solution (supporting electrolyte) (0.1-M KH₂PO₄) was purchased from Merck, and the pH was adjusted using 0.1-M KOH (AG grade, Merck) and H₃PO₄ from Sigma Aldrich at a ratio of 1:1. The solutions were prepared using distilled water. The citrate sol gel method was utilized for the synthesis of the transition-metal-substituted barium ferrite nanomaterials, which were characterized using XRD, SEM, Fourier-transform infrared, and Raman analysis techniques [20 (link)].

Davis minimal medium (DMM), 4-aminoantipyrine, potassium ferrocyanide, catechol, sodium hydroxide pellets used in this study were purchased from HiMedia Laboratories Pvt. Ltd. Mumbai, phenol, 2-mercaptoethanol, ammonium hydroxide, glycerol, disodium EDTA, primers, acids and solvents were procured from Sigma, Aldrich, Tris Base, Agarose from Bio Basic Inc. All the following heavy metals CoCl₂.2H₂O, ZnSO₄.H₂O, (CH₃COO)₂Pb.3H₂O, HgCl₂, CdCl₂, CuSO₄.5H₂O, NiCl₂.6H₂O, MnCl₂.4H₂O, and K₂Cr₂O₇ were purchased from Merck Millipore, Taq DNA polymerase Master Mix from Ampliqon and PCR purification Kit from Favorgen Biotech Corp. were used.