To evaluate contributions of the hypothalamic-pituitary-adrenal (HPA) axis to the glycemic effects of Aβ, adrenalectomy was performed on BRI-Aβ42 mice [B6.Cg-tg(Prnp-ITM2B/APP695*42) A12Emcg/J; stock no. 007182] and WT littermates at 5.5 weeks of age by the Jackson Laboratory (Bar Harbor, ME); unoperated littermates were provided at the same time (6 per group). They were maintained on an ad libitum ND, and adrenalectomized mice were provided a 0.9% saline solution to counter the effects of mineralocorticoid deficiency on kidney function (Bristol and Drill, 1952 (link)). The animals were shipped to the CAVHS facility and allowed to acclimate for one week before testing. In one-week intervals, they underwent GTTs, ITTs, and a GTT in which blood was collected via retro-orbital eye bleed to measure insulin response to glucose challenge at 0 and 30 minutes. The following week all animals were euthanized. One Aβ42-tg adrenalectomized and two WT adrenalectomized mice died during the second GTT. The DetectX Corticosterone Enzyme Immunoassay Kit (Arbor Assays) was used to compare circulating levels of glucocorticoids in serum of adrenalectomized mice at the time of euthanasia.
B6 cg tg prnp itm2b app695 42 a12emcg j
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B6.Cg-tg(Prnp-ITM2B/APP695*42) A12Emcg/J is a transgenic mouse model that expresses the human amyloid precursor protein (APP) with the Swedish (K670N/M671L) and London (V717I) mutations, as well as the human amyloid-β (Aβ) sequence. This model is designed for the study of Alzheimer's disease pathogenesis.
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2 protocols using b6 cg tg prnp itm2b app695 42 a12emcg j
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Adrenalectomy Effects on Aβ-Induced Glycemia
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Adrenalectomy Effects on Aβ-Induced Glycemia
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To evaluate contributions of the hypothalamic-pituitary-adrenal (HPA) axis to the glycemic effects of Aβ, adrenalectomy was performed on BRI-Aβ42 mice [B6.Cg-tg(Prnp-ITM2B/APP695*42) A12Emcg/J; stock no. 007182] and WT littermates at 5.5 weeks of age by the Jackson Laboratory (Bar Harbor, ME); unoperated littermates were provided at the same time (6 per group). They were maintained on an ad libitum ND, and adrenalectomized mice were provided a 0.9% saline solution to counter the effects of mineralocorticoid deficiency on kidney function (Bristol and Drill, 1952 (link)). The animals were shipped to the CAVHS facility and allowed to acclimate for one week before testing. In one-week intervals, they underwent GTTs, ITTs, and a GTT in which blood was collected via retro-orbital eye bleed to measure insulin response to glucose challenge at 0 and 30 minutes. The following week all animals were euthanized. One Aβ42-tg adrenalectomized and two WT adrenalectomized mice died during the second GTT. The DetectX Corticosterone Enzyme Immunoassay Kit (Arbor Assays) was used to compare circulating levels of glucocorticoids in serum of adrenalectomized mice at the time of euthanasia.
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