Chat chr2

Manufactured by Jackson ImmunoResearch

Chat-Chr2 is a laboratory equipment product manufactured by Jackson ImmunoResearch. It is designed for use in research applications. The core function of Chat-Chr2 is to facilitate the analysis and separation of biological samples.

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Lab products found in correlation

Vglut2 cre, by Jackson ImmunoResearch (1 mentions) Imsr jax, by Jackson ImmunoResearch (1 mentions) Chat cre, by Jackson ImmunoResearch (1 mentions) Gad2 cre, by Jackson ImmunoResearch (1 mentions) Drd1 cre, by Jackson ImmunoResearch (1 mentions) C57bi 6j, by Jackson ImmunoResearch (1 mentions) Dat cre, by Jackson ImmunoResearch (1 mentions)

3 protocols using chat chr2

Cre-Mediated Conditional Murine Studies

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All mice in this study were treated in compliance with US Department of Health and Human Services and Baylor College of Medicine IACUC guidelines. Both male and female mice were used in analyses, littermates were randomly assigned to experimental groups, and further specified below. Mice were between 3–5 months of age for surgeries, and between 3–6 months for experiments. Standard mouse chow (Harlan, 2920X) was used for all experiments, and all animals were maintained on a 12 hr light-dark cycle. Depending on the experiment, mice were either group or singly housed (see below). vGlut2-Cre (Slc17a6^tm2(cre)Lowl/J Stock No. 016963) and Chat-Chr2 (Chat-COP4*H134R/EYFP, Slc18a3 6Gfng/J Stock No. 014546) mice were originally purchased and are available from Jackson Laboratories. Genotyping for Cre was done using the following primers: forward 5’-GCATTACCGGTCGATGCAACGAGTGATGAG-3’ and reverse 5’-GAGTGAACGAACCTGGTCGAAATCAGTGCG-3’. Genotyping for Chat-Chr2 was done using the following primers: forward 5’-TCTGTTCCCAGGTCGGCAGC-3’ and reverse 5’-GCAAGGTAGAGCATAGAGGG-3’.

Patel J.M., Swanson J., Ung K., Herman A., Hanson E., Ortiz-Guzman J., Selever J., Tong Q, & Arenkiel B.R. (2019). Sensory perception drives food avoidance through excitatory basal forebrain circuits. eLife, 8, e44548.

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Transgenic Mice for Cholinergic Neural Modulation

Cited 5 times

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The University of Toronto Faculty of Medicine Animal Care Committee approved all experiments in accordance with the guidelines of the Canadian Council on Animal Care (protocol #20011621). We performed experiments on transgenic mice expressing channelrhodopsin in cholinergic afferents (ChAT-ChR2; RRID:IMSR_JAX:014546, Jackson Laboratory [45 (link)]). This transgenic line labels cholinergic neurons and avoids labeling neurons only transiently cholinergic in development [46 (link)]. This approach also avoids use of anesthetic agents known to perturb nicotinic receptors [47 (link), 48 (link)] and surgery known to impact cognitive function [49 (link)]. A total of 60 mice were used for this study (age range: ~P50-350), with balanced numbers of males and females. This age range was selected because mice show progressive, age-dependent changes in behavior through adulthood [33 (link)–35 (link)].

Power S.K., Venkatesan S, & Lambe E.K. (2023). Xanomeline restores endogenous nicotinic acetylcholine receptor signaling in mouse prefrontal cortex. Neuropsychopharmacology, 48(4), 671-682.

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Optimized Mouse Husbandry for Behavioral Studies

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All experiments followed the guidelines for care and use of laboratory animals provided by the National Research Council, and with approved animal protocols from the Institutional Animal Care and Use Committee of the University of Massachusetts Medical School. C57BI/6J (#000664), GAD2-Cre (#010802), Chat-Cre (#006410), DAT-Cre (#006660), Chat-ChR2 (#014546), DRD1-Cre (#028298), and Drdla-tdTomato (#016204) mice were obtained from The Jackson Laboratory, bred in the UMMS animal facility and used in behavioral, optogenetic and biophysical experiments as indicated. Cre lines were crossed with C57BI/6J mice and only heterozygous animals carrying one copy of the Cre recombinase gene were used for experimental purposes. Mice were housed together in cages of no more than five animals and kept on a standard 12 h light/dark cycle (lights ON at 7 A.M.) with ad libitum access to food and water. Three to four weeks before experimentation, subject mice were kept under a reverse 12 h light/dark cycle (lights ON at 7 P. M.) for at least 5 days before any behavioral testing

DeGroot S.R., Zhao-Shea R., Chung L., Klenowski P.M., Sun F., Molas S., Gardner P.D., Li Y, & Tapper A.R. (2020). Midbrain dopamine controls anxiety-like behavior by engaging unique interpeduncular nucleus microcircuitry. Biological psychiatry, 88(11), 855-866.

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