Rpmi 1640 culture medium

Sodium alginate (SA) (from brown algae) (Mol. Wt. 120,000–190,000, viscosity: 5 to 25 cps, M/G ratio: 1.56), glycerol (Gly). PBS tablets, CaCl₂ granules (anhydrous), pen-strep solution (containing 10,000 penicillin units and 10 mg of streptomycin per mL), cellulose acetate membranes (0.45 μm, 25 mm), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide), polystyrene petri dishes (10 cm × 20 mm), and gentamicin sulfate were procured from MilliporeSigma (St. Louis, MO, USA). MRC-5 cells (ATCC CCL-171) were cultured in RPMI-1640 culture medium supplemented with FBS (Nacalai Tesque, Kyoto, Japan). Three bacterial strains S. aureus (ATCC 25923), E. coli (ATCC 8739), and P. aeruginosa (ATCC 9027) were all cultured in Mueller-Hinton broth (MHB) and Agar (Sigma-Aldrich, MO, USA). Arabinoxylan (AX), containing Xylp (72.5%), Araf (21.8%), Rhap (2.3%), and uronic acids (1.4%) was isolated from Plantago ovata (PO) seeds husk (Mol. Wt. 364,470) as reported previously [31 (link),32 (link)].

RPMI-1640 culture medium and antibiotic–antimycotic mixed stock medium (ABAM) were purchased from Nacalai Tesque (Kyoto, Japan). FBS was purchased from Equitech-Bi (Kerrville, TX, USA) and dicholoroacetate (DCA) from Sigma-Aldrich Corporation (Tokyo, Japan).

HTR-8/SVneo cells (subsequently referred to as HTR-8 cells) were obtained from ATCC^® (CRL3271™) and cultured in RPMI-1640 culture medium (Nacalai Tesque, Japan). BeWo cells were obtained from Riken Cell Bank (Ibaraki, Japan) and cultured in Dulbecco’s Modified Eagle Medium/Ham F-12 culture medium (Nacalai Tesque, Japan). The medium was supplemented with 10% fetal bovine serum (FBS), penicillin (100 IU/mL), and streptomycin (100 mg/mL).