Bs 800

We measured red blood cells (RBC), white blood cells (WBC), hemoglobin (Hb), and hematocrit (Hct) using an automatic blood analyzer (TG, Mindray, Shenzhen, China). Serum glucose (Glu), alanine aminotransferase (AST), aspartate aminotransferase (ALT), triglycerides (TC), and cholesterol (TG) were measured using a fully automatic biochemical analyzer (BS-800, Mindray, Shenzhen, China). All kits were purchased from Mindray, Shenzhen, China.
One liver sample was homogenized at a ratio of 9 ml of physiological saline per gram of tissue (100 g/l homogenates) in an ice-water bath and centrifuged at 2,000 g at 4°C for 10 min. The supernatant was then stored in a refrigerator at 4°C. The enzyme-linked immunosorbent assays were used to measure superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), complement C3, lysozyme, glycogen, TG, TC, and protein content, with kits purchased from Shanghai Haoben Biotechnology Co., Ltd. (Shanghai, China). The results were read on a full-band microplate reader (BioTek Eon, Winooski, United States). By measuring the absorbance of each group of samples at different wavelengths, the corresponding sample concentrations could be calculated according to the standard curve of each detection index.

On the 42nd day after ADR administration, rats were anesthetized with IP injection of pentobarbital sodium and blood sample were collected from abdominal aorta in centrifuge tubes and left to clot at room temperature for 1 h. Serum was separated by centrifugation at 3000 rpm for 15 min and stored at −80°C and thawed just before use. Serum levels of total protein (TP), albumin (ALB), globulin (GLB), albumin/globulin (ALB/GLB), total cholesterol (CHOL), triglyceride (TG), creatinine (SCr), urea nitrogen (BUN), cystatin C (Cys-C), sodium (Na), chlorine (CL), potassium (K), and phosphorus (P) were measured with commercial available kits and an automatic biochemistry analyzer (Mindray BS-800, Shenzhen, China).

Venous blood was collected at 7:30-10:30 am, 12 hours after fasting, with serum and plasma separated from blood, which was introduced by our previous publications [17 (link),33 (link),34 (link)]. Then, the serum samples were kept in a -80°C refrigerator for the later biochemical measurement. Glucolipid metabolism indexes were checked by an automatic biochemical analyser (Mindray BS-800), which included serum fasting blood glucose (FBG), total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C). In addition, serum level of renal function indexes of uric acid (UA) and creatinine were measured.

A total of 5971 samples had biochemical index variables. Details of venous blood sample collection and measurement can be found in a previous study [12 (link)]. The serum glycolipid index was measured by an automatic biochemical analyzer (Mindray BS-800), and blood-cell composition was measured by automated hematology analyzers (Sysmex K-4500).

The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transpeptidase (γ-GT), total protein (TP), and the albumin to globulin ratio (A:G) were quantified using a chemistry analyzer BS-800 (Shenzhen Mindray Bio-medical Electronics Co., Ltd., Shenzhen, China).