PCR products corresponding to the 56-bp of WEE1 3’UTR and mutations in the 56-bp of WEE1 3’UTR region were purified and cloned into Promega psiCHECK2 luciferase vector (Promega). MiaPaCa2 cells were transfected with these plasmids in 6-well plates. After 48 h of transfections, cells were treated and luciferase activity was measured with a luciferase assay report kit (Promega) as previously described (32 (link)). For double transfections, cells were first transfected with siRNA HuR or control and 24 h later transfected with luciferase constructs.
Luciferase assay report kit
Manufactured by Promega
The Luciferase Assay Report Kit is a laboratory equipment designed to measure and quantify the activity of the luciferase enzyme. The kit provides the necessary reagents and protocols to perform luciferase-based assays, which are commonly used in research applications to study gene expression, cell viability, and other biological processes.
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Lab products found in correlation
2 protocols using luciferase assay report kit
1
Luciferase Assay for WEE1 3'UTR Mutations
2
Rice Protoplast Isolation and Transformation
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Isolation of rice protoplasts and PEG transformation were performed as described previously (Kawano et al., 2010; Wang et al., 2018) . Protein preparation and luciferasesubstrate interaction were conducted with a Luciferase Assay Report Kit (Promega).
Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash). We used mean values of three independent replications.
Luciferase activity was measured by a microplate reader (Thermo Scientific Varioskan Flash). We used mean values of three independent replications.
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