Rabbit anti hsp60

Manufactured by Abcam

Sourced in United States

Rabbit anti-Hsp60 is a primary antibody that specifically recognizes the Hsp60 protein. Hsp60 is a heat shock protein that functions as a molecular chaperone, assisting in the folding and unfolding of other proteins. This antibody can be used to detect and study the expression and localization of Hsp60 in various biological samples.

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Lab products found in correlation

Rabbit anti tom20, by Santa Cruz Biotechnology (1 mentions) Rabbit anti lc3b, by Cell Signaling Technology (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Mouse anti vdac1, by Abcam (1 mentions) Mouse anti tom70, by Santa Cruz Biotechnology (1 mentions) Mouse anti mfn2, by Abcam (1 mentions) Mouse anti actin, by Abcam (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Rabbit anti phospho eif2a, by Cell Signaling Technology (1 mentions) Mouse anti grp78, by R&D Systems (1 mentions) Rabbit anti mtor, by Cell Signaling Technology (1 mentions) Anti tom20, by Santa Cruz Biotechnology (1 mentions) Anti drp1, by BD (1 mentions) Mitoprofile total oxphos antibody cocktail, by Abcam (1 mentions) Rabbit anti gapdh, by Abcam (1 mentions) Bca protein assay, by Thermo Fisher Scientific (1 mentions) Anti mfn2, by Abcam (1 mentions) Rabbit anti phospho mtor ser2448, by Cell Signaling Technology (1 mentions) Anti mnsod, by Abcam (1 mentions)

Spelling variants of this product

HSP60 (31 citations) Anti-Hsp60 (21 citations) Rabbit anti- (5 citations)

3 protocols using rabbit anti hsp60

Antibody Validation for Protein Analysis

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The following primary antibodies were used in our study: mouse anti-CValpha (Abcam, 14748; 1:5000), rabbit anti-Hsp60 (Abcam, 46798; 1:500), rabbit anti-ROCK2 (Abcam, 125025; 1:5000), mouse anti-actin (Abcam, 8226; 1:1000), mouse anti-HK2 (Abcam, 3740910; 1:1000), mouse anti-Mfn2 (Abcam, 56889; 1:1000), rabbit anti-TOM20 (Santa Cruz, 191883; 1:1000), mouse anti-VDAC1 (Abcam, 14734; 1:1000), mouse anti-TOM70 (Santa Cruz, 390545; 1:1000), mouse anti-UQCRC2 (Abcam, 14745, 1:1000), rabbit anti-COXIV (Novus, NB110-39115; 1:500) and mouse anti-Flag (Sigma, F1804; 1:1000). Rabbit and mouse horseradish peroxidase-conjugated secondary antibodies (Jackson Immunoresearch; 1:5000) were used for secondary incubation.
The following primary antibodies were used in our study for immunofluorescence: mousen anti-CValpha (Abcam, 14748; 1:500), rabbit anti-Hsp60 (Abcam, 46798; 1:500), rabbit anti-LC3B (Cell Signaling, 2775) and mouse anti-Flag (Sigma, F1804; 1:1000). All antibodies were diluted in 1% goat serum.

Moskal N., Riccio V., Bashkurov M., Taddese R., Datti A., Lewis P.N, & Angus McQuibban G. (2020). ROCK inhibitors upregulate the neuroprotective Parkin-mediated mitophagy pathway. Nature Communications, 11, 88.

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Comprehensive Western Blotting Protocol

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Western blotting was conducted according to standard protocols. The antibodies used in this study were as follows: rabbit anti-WWOX (a kind gift of Dr. M Aldaz, MD Anderson Cancer Centre, Houston, TX, USA) and ab137726 antibody (abcam), rabbit anti-β-actin (no. 4970; Cell Signalling Technology, Danvers, MA, USA), rabbit anti-Hsp60 (abcam; ab46798), rabbit anti-cleaved (Asp175) caspase-3 (R&D Systems, Minneapolis, MN, USA; 269518), mouse caspase-2 (no. 2224; Cell Signalling Technology), rabbit caspase-7 (no. 9492; Cell Signalling Technology), rabbit anti-phospho-eIF2A (Cell Signalling Technology; 9721), mouse anti-GRP78 (474421; R&D Systems), mouse anti-JNK1/JNK2 252323, MAB2076 and rabbit AF1205 (phospho-JNK) (R&D Systems), rabbit anti-phospho (T183/Y185)-JNK (AF1205), rabbit IRE-1 (ab48187 and ab37073; abcam) and PERK (no. 3192; Cell Signalling Technology).

Janczar S., Nautiyal J., Xiao Y., Curry E., Sun M., Zanini E., Paige A.J, & Gabra H. (2017). WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response. Cell Death & Disease, 8(7), e2955-.

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Quantifying Mitochondrial Function Markers

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Western blotting was performed as described (Zhu et al., 2007 (link)). Protein concentration was determined using a BCA protein assay (Thermo Fisher Scientific, Rockford, IL), and equal amounts of protein (25 μg) were added to each well. The following primary antibody dilutions were used: 1:5,000 mouse monoclonal MitoProfile Total OXPHOS antibody cocktail (MitoSciences, Eugene, OR), 1:1,000 rabbit anti-FNDC5 (Abcam, Cambridge, MA), 1:1,000 rabbit anti-PGC-1α (Santa Cruz, Dallas, TX), 1:10,000 rabbit anti-TFAM (courtesy of Dr. Craig Cameron, Penn State University), 1:1,1000 rabbit anti-HSP60 (Abcam), 1:1,000 rabbit anti-BDNF (Abcam), 1:500 mouse monoclonal anti-LC3 (5F10 clone, Nanotools, Teningen, Germany), 1:1,000 rabbit anti-Phospho-mTOR (Ser2448, Cell Signaling, Danvers, MA), 1:1,000 rabbit anti-mTOR (Cell Signaling, Danvers, MA), 1:1000 anti-DRP1 (BD Biosciences, Franklin Lakes, NJ), 1:1000 anti-SIRT3 (courtesy of Dr. Eric Verdin, UCSF, CA), 1:2,000 anti-MFN2 (Abcam), 1:5,000 anti-TOM20 (Santa Cruz), and 1:1,000 anti-MnSOD (Abcam) followed by ECL detection (GE Healthcare, Little Chalfont, UK). Membranes were stripped and reprobed with 1:10,000 rabbit anti-GAPDH (Abcam). Densitometry was performed using ImageJ’s Gel analyzer (NIH, Bathesda, MD).

Gusdon A.M., Callio J., DiStefano G., O’Doherty R.M., Goodpaster B.H., Coen P.M, & Chu C.T. (2017). Exercise Increases Mitochondrial Complex I Activity and DRP1 Expression in the Brains of Aged Mice. Experimental gerontology, 90, 1-13.

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