Annexin 5 pi double staining kit
The Annexin V/PI double staining kit is a laboratory reagent used for the detection and analysis of apoptosis in cell samples. It provides a method for identifying early and late apoptotic cells through the simultaneous measurement of Annexin V and propidium iodide (PI) binding.
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2 protocols using annexin 5 pi double staining kit
Annexin V/PI Apoptosis Assay
Apoptosis and Cell Cycle Analysis
To analyze DNA content and cell cycle by flow cytometry, the cells were harvested, washed twice with PBS, and fixed with 70% ethanol. Immediately before flow cytometric analysis, the cells were washed twice in PBS and then stained for DNA content using 0.5 ml of 400 µl/ml PI (Sigma, USA) and 100 μg/ml RNAase A (Sigma, USA) in PBS and 38 mM sodium citrate pH 7.4. Data were again collected on a Becton Dickinson FACS Canto II Flow Cytometer.
Cell culture and L-leucine/L-glutamine uptake assays TE-1 cells were cultured in RPMI-1640 medium containing 10% v/v FBS, penicillin-streptomycin solution, and 1 mM sodium pyruvate and maintained at 37°C in 5% CO 2 [ 3 H].-L-leucine and [ 3 H]-L-glutamine uptake assays were performed as described previously [18, 19] .
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