Ab195192

To observe the cell morphology, raw264.7 macrophages were cultured with 20 μg/mL Da-g-Xan for 4 h and then washed with PBS three times. Then, cells were fixed with 4% (w/v) paraformaldehyde for 30 min at room temperature, followed by 0.05% (w/v) Triton X-100 for permeabilization. Cytoplasm was stained with β-actin (abcam, ab115777) followed by donkey anti-rabbit antibodies (abcam, ab150075), and nuclei were stained with DAPI (abcam, ab104139). To co-localize CD206 with Da-g-Xan, raw264.7 macrophages were cultured with 20 μg/mL of Da-g-Xan-FITC for 4 h followed by staining with CD206 (abcam, ab195192). The nuclei were also stained with DAPI (abcam, ab104139). A confocal scanning microscope (FV1000, Olympus, Japan) was used for image analysis. The colonic anastomosis samples were fixed with 4% (w/v) paraformaldehyde, embedded in Paraffin, and then mounted on slides. The slides were treated with CD 31 (Servicebio, GB13063) and α-SMA (Servicebio, GB13044) followed by staining with donkey anti-goat antibodies (abcam, ab150135) and goat anti-mouse antibodies (Servicebio, GB22301). The images were recorded by an inverted fluorescence microscope (AXIO, ZEISS, Germany).