Recombinant human EGF (rhEGF) was purchased from PeproTech (Cranbury, NJ, USA). LY294002, PD98059 and Rapamycin were from Calbiochem (San Diego, CA, USA). Cells were treated with rhEGF (10 ng/ml) and LY294002 (10 μM), PD98059 (50 μM) or Rapamycin (10 μM) for various times as noted in the text.
Recombinant human egf rhegf
Manufactured by Thermo Fisher Scientific
Sourced in United States
Recombinant human EGF (rhEGF) is a laboratory reagent that is a recombinant form of the human epidermal growth factor protein. It is used in cell culture applications to promote cell growth and proliferation.
Automatically generated - may contain errors
2 protocols using recombinant human egf rhegf
1
EGF Signaling Pathway Modulation
2
Isolation of Prostate Epithelial Cells from Genetically-Modified Mice
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Whole prostate gland including anterior, dorsolateral and ventral lobes were harvested from 8 -12 weeks old Trp53 -/-; Rb f/f , Trp53 -/-; Pten f/f or Trp53 -/-; Rb f/f ; Pten f/f male mice, and digested with one part 10x Collagenase/Hyaluronidase (#07912, STEMCELL Technologies) and nine parts DMEM/F-12 (#36254, STEMCELL Technologies) supplemented with 5% FBS (#SH30079, HyClone) for 3 hrs at 37 °C. The resultant organoids were further digested in 0.25% trypsin-EDTA (1 hr), 5 mg/ml Dispase and 100 μg/ml DNase I (1 min), and then filtered through a 40 μm cell strainer. Lineage positive hematopoietic, endothelial and fibroblast cells (CD45 + ; CD31 + ; Ter119 + ; BP-1 + ) were removed using EasySep Mouse Epithelial Cell Enrichment Kit (#19758, STEMCELL Technologies). The resulting epithelial cells were maintained in FBS-free ProstaCult TM Medium (#05640, STEMCELL Technologies) supplemented with 10 ng/mL recombinant human bFGF (rh bFGF) (PeproTech), 10 ng/mL recombinant human EGF (rh EGF) (PeproTech) and 4 μg/mL Heparin (#07980, STEMCELL Technologies), and plated onto collagen coated dishes (Iwaki).
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