MTT assay was applied to measure the proliferation of ALL cells. The treated cell line (5 × 103 cells/100 μl) was plated into a 96-well plate. After 24, 48 and 72 h, the cell proliferation was evaluated with sterile MTT solution (Beyotime). Spectrophotometer (Molecular Devices, Sunnyvale, CA, USA) was utilized to observe and record the optical density at 570 nm.
Sterile mtt solution
Manufactured by Beyotime
Sterile MTT solution is a laboratory reagent used in colorimetric assays. It serves as an indicator of cellular metabolic activity, allowing researchers to assess cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using sterile mtt solution
1
MTT Assay for Cell Proliferation
2
Proliferation of T98G and U251 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The proliferation of T98G and U251 cells (5 × 103/100 μL) was measured by MTT assay. Each processing consisted of three duplicates. After 24 h of cell culture, 48, 72, 96, and 120 h, sterile MTT solution (Beyotime) was used to assess cell proliferation. Absorbance at 450 nm wavelength was assayed with a microplate reader (Sunnyvale, CA, USA).
3
Cell Viability Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MTT method was implemented to examine cell viability in proliferation. HO-8910PM cells were seeded into 96-well plates at a density of 5 × 103 cells/well, and each treatment was run in triplicate. After 1 d, 2 d, 3 d, 4 d and 5 d, respectively, sterile MTT solution (Beyotime) was added to the cells per the instructions. The absorbance value at 490 nm was measured by an enzyme-labeled instrument (Molecular Devices, Sunnyvale, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!