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Macs ms system

Manufactured by Miltenyi Biotec
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The MACS MS system is a magnetic cell separation instrument designed for the isolation and enrichment of target cells from complex samples. It utilizes magnetic microbeads conjugated to specific antibodies or ligands to label and magnetically isolate the desired cell population. The system provides a gentle and efficient method for cell separation, enabling the purification of cells for downstream applications.

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Lab products found in correlation

Rneasy micro kit, by Qiagen (3 mentions) Superscript vilo cdna synthesis kit and master mix, by Thermo Fisher Scientific (2 mentions) Power sybr green pcr master mix, by Thermo Fisher Scientific (2 mentions) Dnase 1, by Merck Group (2 mentions) Collagenase dispase, by Merck Group (2 mentions) Avertin, by Merck Group (2 mentions) 70 μm cell strainer, by BD (2 mentions)

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MACS system (192 citations)

6 protocols using macs ms system

1

Isolation of Cerebellar Endothelial Cells

Cited 1 time
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At the specified time points, cerebellar endothelial cells were isolated by mechanical and enzymatic digestion followed by separation using magnetic-activated cell sorting by anti-CD31/PECAM1-conjugated magnetic beads (MACS MS system, Miltenyi Biotec), as previously described2 (link).
Ren A.A., Snellings D.A., Su S.Y., Hong C.C., Castro M., Tang A.T., Detter M.R., Hobson N., Girard R., Romanos S., Lightle R., Moore T., Shenkar R., Benavides C., Beaman M.M., Mueller-Fielitz H., Chen M., Mericko P., Yang J., Sung D.C., Lawton M.T., Ruppert M., Schwaninger M., Körbelin J., Potente M., Awad I.A., Marchuk D.A, & Kahn M.L. (2021). PIK3CA and CCM mutations fuel cavernomas through a cancer-like mechanism. Nature, 594(7862), 271-276.
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2

Isolation and Quantification of Endothelial Cells

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At the specified time points, cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting by anti-CD31 conjugated magnetic beads (MACS MS system, Miltenyl Biotec), as previously described9 (link). Lung endothelial cells were isolated through enzymatic digestion as previously described followed by separation using anti-CD31 conjugated magnetic beads and the MACS MS system48 . Isolated endothelial cells were pelleted and total RNA was extracted using the RNeasy Micro kit (Qiagen 74004). For qPCR analysis, cDNA was synthesized from 300 ng to 500 ng total RNA using the SuperScriptTM VILO cDNA Synthesis Kit and Master Mix (Thermo Fisher 11755050). Real-time PCR was performed with Power SYBR Green PCR Master Mix (Thermo Fisher 4368577) using the primers listed:
Tang A.T., Choi J.P., Kotzin J.J., Yang Y., Hong C.C., Hobson N., Girard R., Zeineddine H.A., Lightle R., Moore T., Cao Y., Shenkar R., Chen M., Mericko P., Yang J., Li L., Tanes C., Kobuley D., Võsa U., Whitehead K.J., Li D.Y., Franke L., Hart B., Schwaninger M., Henao-Mejia J., Morrison L., Kim H., Awad I.A., Zheng X, & Kahn M.L. (2017). Endothelial TLR4 and the microbiome drive cerebral cavernous malformations. Nature, 545(7654), 305-310.
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3

Isolation and Analysis of Cerebellar Endothelial Cells

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Cerebellar endothelial cells were isolated through enzymatic digestion, followed by separation using magnetic-activated cell sorting by anti-CD31–conjugated magnetic beads (MACS MS system, Miltenyi Biotec), as previously described (6 (link)). Isolated cerebellar endothelial cells were pelleted, and total RNA was extracted using the RNeasy micro kit (Qiagen 74004). PCR reactions were set up in triplicate, and data shown are from at least three independent repeats. Changes in gene expression were detected using the following mouse primers: Gapdh, 5′-GTCCCGTAGACAAAATGGTGA-3′ (forward) and 5′-TTTGATGTTAGTGGGGTCTCG-3′ (reverse); Klf2, 5′-CGCCTCGGGTTCATTTC-3′ (forward) and 5′-AGCCTATCTTGCCGTCCTTT-3′ (reverse); Klf4, 5′-GTGCCCCGACTAACCGTTG-3′ (forward) and 5′-GTCGTTGAACTCCTCGGTCT-3′ (reverse); eNos, 5′-CCTGTGCATGGATGAGTATGA-3′ (forward) and 5′-TGAGCAGGAGACACTGTTGAA-3′ (reverse); and Id1, 5′-ATCCTGCAGCATGTAATCGAC-3′ (forward) and 5′-GAGTCCATCTGGTCCCTCAGT-3′ (reverse).
Choi J.P., Wang R., Yang X., Wang X., Wang L., Ting K.K., Foley M., Cogger V., Yang Z., Liu F., Han Z., Liu R., Baell J, & Zheng X. (2018). Ponatinib (AP24534) inhibits MEKK3-KLF signaling and prevents formation and progression of cerebral cavernous malformations. Science Advances, 4(11), eaau0731.
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4

Isolation of Cerebellar Endothelial Cells

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Cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting (MACS MS system, Miltenyi Biotec). Mice were first anesthetized with Avertin (Sigma Aldrich, T48402) and perfused with sterile phosphate buffered saline (PBS). Cerebella of the mice were then digested by 1 mg/ml collagenase/dispase (Sigma) and 0.02 mg/ml DNase I (Sigma) in complete DMEM for 10 min at 37 °C with gentle shaking. The digestion was then passed through a 70 μm cell strainer (BD Biosciences). Cells were centrifuged, resuspended, and incubated with anti-mouse CD31 antibody conjugated microbeads for 15 min at 4 °C. Microbead-bound cells were then washed and separated using MACS MS columns according to vendor protocol. Cells bound to the magnetic column were eluted and centrifuged for downstream applications, including western blotting and qPCR analysis.
Zhou Z., Tang A.T., Wong W.Y., Bamezai S., Goddard L.M., Shenkar R., Zhou S., Yang J., Wright A.C., Foley M., Arthur J.S., Whitehead K.J., Awad I.A., Li D.Y., Zheng X, & Kahn M.L. (2016). Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signaling. Nature, 532(7597), 122-126.
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5

Isolation and Quantification of Endothelial Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
At the specified time points, cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting by anti-CD31 conjugated magnetic beads (MACS MS system, Miltenyl Biotec), as previously described9 (link). Lung endothelial cells were isolated through enzymatic digestion as previously described followed by separation using anti-CD31 conjugated magnetic beads and the MACS MS system48 . Isolated endothelial cells were pelleted and total RNA was extracted using the RNeasy Micro kit (Qiagen 74004). For qPCR analysis, cDNA was synthesized from 300 ng to 500 ng total RNA using the SuperScriptTM VILO cDNA Synthesis Kit and Master Mix (Thermo Fisher 11755050). Real-time PCR was performed with Power SYBR Green PCR Master Mix (Thermo Fisher 4368577) using the primers listed:
Tang A.T., Choi J.P., Kotzin J.J., Yang Y., Hong C.C., Hobson N., Girard R., Zeineddine H.A., Lightle R., Moore T., Cao Y., Shenkar R., Chen M., Mericko P., Yang J., Li L., Tanes C., Kobuley D., Võsa U., Whitehead K.J., Li D.Y., Franke L., Hart B., Schwaninger M., Henao-Mejia J., Morrison L., Kim H., Awad I.A., Zheng X, & Kahn M.L. (2017). Endothelial TLR4 and the microbiome drive cerebral cavernous malformations. Nature, 545(7654), 305-310.
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6

Isolation of Cerebellar Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cerebellar endothelial cells were isolated through enzymatic digestion followed by separation using magnetic-activated cell sorting (MACS MS system, Miltenyi Biotec). Mice were first anesthetized with Avertin (Sigma Aldrich, T48402) and perfused with sterile phosphate buffered saline (PBS). Cerebella of the mice were then digested by 1 mg/ml collagenase/dispase (Sigma) and 0.02 mg/ml DNase I (Sigma) in complete DMEM for 10 min at 37 °C with gentle shaking. The digestion was then passed through a 70 μm cell strainer (BD Biosciences). Cells were centrifuged, resuspended, and incubated with anti-mouse CD31 antibody conjugated microbeads for 15 min at 4 °C. Microbead-bound cells were then washed and separated using MACS MS columns according to vendor protocol. Cells bound to the magnetic column were eluted and centrifuged for downstream applications, including western blotting and qPCR analysis.
Zhou Z., Tang A.T., Wong W.Y., Bamezai S., Goddard L.M., Shenkar R., Zhou S., Yang J., Wright A.C., Foley M., Arthur J.S., Whitehead K.J., Awad I.A., Li D.Y., Zheng X, & Kahn M.L. (2016). Cerebral cavernous malformations arise from endothelial gain of MEKK3-KLF2/4 signaling. Nature, 532(7597), 122-126.
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