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710 confocal microscope

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The 710 confocal microscope is a laboratory equipment designed for high-resolution imaging of samples. It utilizes a focused laser beam and a pinhole aperture to capture detailed optical sections of a specimen, enabling the visualization of intricate structures and features.

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Lab products found in correlation

Toto 3 iodide, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit fitc, by Jackson ImmunoResearch (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions)

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Confocal microscope (2 citations) LSM 710 Meta confocal microscope (2 citations)

3 protocols using 710 confocal microscope

1

Immunohistochemical Staining of A. aegypti Brain

Cited 2 times
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Immunohistochemical staining was performed as described earlier [6 (link)],[23 (link)],[27 ]. Rat anti-DN-cadherin developed by T. Uemura was obtained from the Developmental Studies Hybridoma Bank, which was created by the NICHD-NIH and is maintained at the University of Iowa, and was used at a concentration of 1:50 to visualize neuropile domains in the A. aegypti brain. Alexa Fluor 568 goat anti-rat IgG secondary antibody (Life Technologies) was used at a concentration of 1:200. Nuclear counterstaining was performed with TOTO-3 iodide (Molecular Probes, Grand Island, NY, USA). Double labeling immunohistochemical experiments combined with in situ hybridization were performed with gene-specific probes in conjunction with anti-HRP (Jackson Immunoresearch) detected with goat anti-rabbit FITC (Jackson Immunoresearch) as described in Haugen et al. [22 (link)]. Imaging of fluorescently stained specimens was performed with a Zeiss 710 confocal microscope using Zen software, and scanned images were analyzed using FIJI and Adobe Photoshop software.
Tomchaney M., Mysore K., Sun L., Li P., Emrich S.J., Severson D.W, & Duman-Scheel M. (2014). Examination of the genetic basis for sexual dimorphism in the Aedes aegypti (dengue vector mosquito) pupal brain. Biology of Sex Differences, 5, 10.
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2

Mosquito Brain Immunohistochemistry Quantification

Cited 1 time
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Three biological replicate immunohistochemical staining experiments were performed on the brains of control or dop1.462 IRP-treated adult or L4 mosquitoes as described (Clemons et al., 2010c (link); Mysore et al., 2011 (link)). The following reagents were used in these studies: anti-Bruchpilot mAb nc82 antibody (Wagh et al., 2006 (link)) (Developmental Studies Hybridoma Bank, Iowa City, Iowa, Product nc82, which was deposited by E. Buchner) and TO-PRO-3 iodide (Molecular Probes, Eugene, OR). Three biological replicate experiments were performed on the brains of 20 L4 larvae or adults per control or experimental treatment in each biological replicate experiment. Processed brain tissues were mounted and imaged using a Zeiss 710 confocal microscope and Zen software, and the images were analyzed with Adobe Photoshop CC 2018 and FIJI ImageJ (Schindelin, 2019 ) software. This permitted quantification of mean gray values, which were calculated and statistically analyzed using Student’s t-test as described (Mysore et al., 2015 (link)).
Hapairai L.K., Mysore K., Sun L., Li P., Wang C.W., Scheel N.D., Lesnik A., Scheel M.P., Igiede J., Wei N., Severson D.W, & Duman-Scheel M. (2020). Characterization of an adulticidal and larvicidal interfering RNA pesticide that targets a conserved sequence in mosquito G protein-coupled dopamine 1 receptor genes. Insect biochemistry and molecular biology, 120, 103359.
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3

Fluorescent Labeling of Cell Nuclei

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Alexa 488 1:200 and Alexa 594 1:100 (Invitrogen). Nuclei were counterstained with DAPI. Confocal images were collected using a Zeiss 710 confocal microscope and processed with Adobe Photoshop CS.
Cordero J.B., Ridgway R.A., Valeri N., Nixon C., Frame M.C., Muller W.J., Vidal M, & Sansom O.J. (2014). c-Src drives intestinal regeneration and transformation. The EMBO Journal, 33(13), 1474-1491.
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