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Anti pom121

Manufactured by Thermo Fisher Scientific
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Anti-Pom121 is a laboratory equipment product designed for use in scientific research. It serves as a tool for the detection and analysis of the Pom121 protein, which is involved in various cellular processes. The core function of Anti-Pom121 is to facilitate the identification and quantification of Pom121 in biological samples, supporting researchers in their investigations.

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Lab products found in correlation

Anti lamin b1, by Abcam (2 mentions) Anti lamin a, by Merck Group (2 mentions) Anti nup210, by Fortis Life Sciences (2 mentions) Anti nup88, by BD (2 mentions) Anti nup107, by Cell Signaling Technology (2 mentions) Anti nup98, by Cell Signaling Technology (2 mentions) Anti nup93 f2, by Santa Cruz Biotechnology (2 mentions) Anti cav2, by BD (2 mentions) Anti cav1, by Cell Signaling Technology (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Sp8 confocal microscope, by Leica (2 mentions) Mab414, by Fortrea (2 mentions) Vectashield, by Vector Laboratories (2 mentions) Anti phospho histone h3 s10, by Cell Signaling Technology (1 mentions) Anti calnexin, by Abcam (1 mentions)

3 protocols using anti pom121

1

Antibodies Used in Nuclear Pore Complex Study

Cited 1 time
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The following commercial antibodies were used in this study: anti‐Nup62 (rabbit; Invitrogen, PA5‐21882), anti‐CKAP4/Climp63 (rabbit; Proteintech, 16686‐1‐AP; Fig 2), anti‐Calnexin (rabbit; Abcam, ab22595), anti‐Climp63 (mouse; Enzo lifesciences, ALX‐804‐604; Figs 5 and EV2), anti‐CREST (human; ImmunoVision, HCT‐0100), anti‐phospho‐Histone H3(S10) (rabbit; Cell Signaling Technology, 9701S), anti‐RTN4/NOGO (rabbit; Proteintech, 10950‐1‐AP), and anti‐POM121 (rabbit; ThermoFisher Scientific, PA5‐85161). Rabbit antibodies against GP210, Nup160, Nup133, Nup107, Nup96, Nup53 and Nup214 have been previously described (Mansfeld et al, 2006 (link); Laurell et al, 2011 (link); Linder et al, 2017 (link)).
Gandhimathi R., Pinotsi D., Köhler M., Mansfeld J., Ashiono C., Kleele T., Pawar S, & Kutay U. (2023). Super‐resolution microscopy reveals focal organization of ER‐associated Y‐complexes in mitosis. EMBO Reports, 24(9), e56766.
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2

Immunofluorescence Staining of Primary Liver Cells

Cited 1 time
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Primary liver cells fixed in 4 methanol-free % PFA for 5 minutes were blocked and stained in IF buffer (1x PBS, 10 mg/ml BSA, 0.02% SDS, 0.1% Triton X-100). Primary and secondary antibodies was incubated for 1 hour at RT or overnight at 4°C with IF buffer washes between and after incubations. Cells were labeled with Hoechst 33342 (Life Technologies) for 5 minutes before mounting using VECTASHIELD (Vector Laboratories). Antibodies used: mAb414 (Covance or BioLegend); anti-Nup210 (Bethyl Laboratories, Inc.); anti-Pom121 (ThermoFisher Scientific); anti-Nup88 (22, BD Transduction Laboratories); anti-Nup107 (a kind gift from Martin Hetzer9 (link), The Salk Institute, La Jolla CA, USA); anti-Nup98 (39A3, Cell Signaling Technology); anti-Nup93 (F2, Santa Cruz BioTechnology); anti-Lamin A (Sigma Aldrich); anti-Lamin B1 (Abcam); anti-Cav1 (Cell Signaling Technology), and anti-Cav2 (65, BD Biosciences). Images were taken using a Leica SP8 confocal microscope and analyzed using the Leica Application Suite X software v3.1.5.16308, ImageJ v2.0.0-rc-54/1.51h (NIH), and Adobe Photoshop CS5.1 v12.1 x64.
Borlido J., Sakuma S., Raices M., Carrette F., Tinoco R., Bradley L.M, & D’Angelo M.A. (2018). Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nature immunology, 19(6), 594-605.
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3

Immunofluorescence Staining of Primary Liver Cells

Cited 1 time
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Primary liver cells fixed in 4 methanol-free % PFA for 5 minutes were blocked and stained in IF buffer (1x PBS, 10 mg/ml BSA, 0.02% SDS, 0.1% Triton X-100). Primary and secondary antibodies was incubated for 1 hour at RT or overnight at 4°C with IF buffer washes between and after incubations. Cells were labeled with Hoechst 33342 (Life Technologies) for 5 minutes before mounting using VECTASHIELD (Vector Laboratories). Antibodies used: mAb414 (Covance or BioLegend); anti-Nup210 (Bethyl Laboratories, Inc.); anti-Pom121 (ThermoFisher Scientific); anti-Nup88 (22, BD Transduction Laboratories); anti-Nup107 (a kind gift from Martin Hetzer9 (link), The Salk Institute, La Jolla CA, USA); anti-Nup98 (39A3, Cell Signaling Technology); anti-Nup93 (F2, Santa Cruz BioTechnology); anti-Lamin A (Sigma Aldrich); anti-Lamin B1 (Abcam); anti-Cav1 (Cell Signaling Technology), and anti-Cav2 (65, BD Biosciences). Images were taken using a Leica SP8 confocal microscope and analyzed using the Leica Application Suite X software v3.1.5.16308, ImageJ v2.0.0-rc-54/1.51h (NIH), and Adobe Photoshop CS5.1 v12.1 x64.
Borlido J., Sakuma S., Raices M., Carrette F., Tinoco R., Bradley L.M, & D’Angelo M.A. (2018). Nuclear pore complex-mediated modulation of TCR signaling is required for naïve CD4+ T cell homeostasis. Nature immunology, 19(6), 594-605.
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