Leuconostoc mesenteroids
Leuconostoc mesenteroids is a gram-positive, catalase-negative, heterofermentative bacterium. It is commonly used in the production of various fermented foods and beverages. This organism plays a role in the conversion of sugars, producing lactic acid and carbon dioxide as byproducts.
Lab products found in correlation
2 protocols using leuconostoc mesenteroids
Synthesis and Characterization of Biotin-Dextran Conjugates
Synthesis of Amino-Dextran Conjugates
as previously reported.35 (link) 500 mg of dextran
(average molecular weight 35 000–45 000, from Leuconostoc mesenteroids, Sigma-Aldrich) was dissolved in
anhydrous DMSO in 50 mL tube. The solution was warmed to 50 °C
to completely dissolve the dextran. 126 mg of N,N′-carbonyldiimidazole in 500 μL of anhydrous
DMSO was added and the solution was incubated with gentle shaking
at 50 °C for 20 min. After the incubation, 250 μL of ethylenediamine
was added and the solution was incubated with gentle shaking at 50
°C for 22 h. 25 mL of acetone was added and the solution was
cooled on ice for 15 min. The tube was centrifuged at 700g for 10 min and the supernatant was removed. The pellet was resuspended
in 20 mL of acetone and the tube was centrifuged at 700g for 10 min. The supernatant was removed and the pellet was air-dried.
The dried pellet was dissolved in 10 mL of ultrapure water and the
solution was dialyzed with Snakeskin dialysis tubing (7 MWCO, Thermo
Scientific) for 2 days. The dialyzed dextran was lyophilized and used
for peptoid/peptide conjugation. The extent of amine derivatization
was determined by a colorimetric test using 2,4,6-trinitrobenzenesulfonic
acid (TNBS).43 (link)
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