A self-build imaging module was attached to standard surgical laparoscope (#49003 AA, HOPKINS II Straight Forward Telescope 0°, Karl Storz, El Segundo, CA, USA) to collect WL and NIR FL images. WL illumination (MCWHL5, Thorlabs, Newton, NJ, USA) and FL excitation source (λex = 785 nm, #iBEAM-SMART-785-S, Toptica Photonics) were coupled into the laparoscope. WL and NIR FL images are collected, simultaneously, by a color CCD camera (#GX-FW-28S5C-C, Point Gray Research, Richmond, BC V6W 1K7, Canada) and a NIR CCD camera (Orca R-2, Hamamatsu Photonics, Hamamatsu City, Shizuoka Pref., Japan) with a laser power of 1.2 mW, respectively.
Mcwhl5
Manufactured by Thorlabs
Sourced in United States
The MCWHL5 is a multi-channel white LED light source from Thorlabs. It features five individual LED channels that can be controlled independently. The MCWHL5 is designed for use in a variety of applications where adjustable, uniform white illumination is required.
Automatically generated - may contain errors
Lab products found in correlation
Hopkins 2 straight forward telescope 0, by Storz (2 mentions)
Ledd1b, by Thorlabs (2 mentions)
Orca r2, by Hamamatsu Photonics (1 mentions)
Ibeam smart 785 s, by Toptica (1 mentions)
Am4815ztl, by Dino-Lite (1 mentions)
M plan apo, by Mitutoyo (1 mentions)
Fg105lca, by Thorlabs (1 mentions)
Cld1015, by Thorlabs (1 mentions)
Kdc101, by Thorlabs (1 mentions)
Dcc1545m, by Thorlabs (1 mentions)
7 protocols using mcwhl5
1
Multimodal Laparoscopic Imaging System
2
Multi-Modal Microscopy for Cell Targeting
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Two separate visualization systems were used, one for monitoring the fiber tip to cell distance and the other for cell targeting. The CTM was positioned at a right angle from the sample surface and above the electrospray and MS inlet orifice axis. Brightfield illumination was provided by a white LED with 6500 K color temperature (MCWHL5, Thorlabs, Newton, NJ, United States) with its intensity adjusted by a LED driver (LEDD1B, Thorlabs, Newton, NJ, United States). For uniform lighting, an aspheric condenser lens with a diffuser surface (ACL2520-DG15-A, Thorlabs, Newton, NJ, United States) was installed. Since the illumination was perpendicular to the sample surface and the camera, a 30:70 (reflection:transmission) beamsplitter (BSS10R, Thorlabs, Newton, NJ, United States) was inserted into the optical train. For cell targeting, a 20× infinity-corrected objective lens (M Plan Apo, Mitutoyo Co., Kanagawa, Japan) was combined with a 1× tube lens and a 4-megapixel monochrome CCD camera (4070-GE, Thorlabs, Newton, NJ, United States). A long working distance fiber-monitoring microscope (FMM) (AM4815ZTL, Dino-lite, Torrance, CA, United States) with an extended magnification range (5× to 140×) was positioned at a 20° elevation angle to the sample surface.
3
Optical Fiber-based AuNPs Characterization
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The optical set-up used in this work consisted of a LED (MCWHL5, Thorlabs) as the light source in the 400–700 nm range (which matches the resonance wavelength of the AuNPs used), a fiber optical coupler (FOC) and a mini-spectrometer (Avantes, mini2048-VI25) (Fig. SI3 ). In order to study the effect of the core size in the obtained signal, three different fibers were used with their correspondent couplers. Two multimode fibers (MMF) with a core diameter of 105 µm and 50 µm (FG105LCA and FG050LCA, Thorlabs). Herein, such fibers will be denoted as 105MMF and 50MMF, respectively. The FOCs for the 105MMF and 50MMF were, respectively, a TM105R5S1A and TM50R5S2A purchased from Thorlabs. The other fiber used was a single mode fiber (SMF) in the visible range with a core diameter of 2.5 µm (460HP, Thorlabs) with a FOC (TW560R5F2, Thorlabs).
All the optical fibers were cleaned and cleaved using an optical fiber cleaver (VF-78, INNO Instrument America).
All the optical fibers were cleaned and cleaved using an optical fiber cleaver (VF-78, INNO Instrument America).
4
Optical Tweezers Setup with Laser Diode
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A 785 nm laser diode (FPL785S-250, Thorlabs, Ely, UK) with maximum output of 250 mW and controlled via customized LabVIEW software using a controller (CLD1015, Thorlabs) was used in an optical tweezers assembly, as shown in Figure 1 . The beam is directed via a dichroic mirror (DM) into the back aperture of a Nikon ×100, 1.3 NA objective lens (Thorlabs). A microscope stage is mounted on a Three-Axis Motorized Translation Stage (MT3/M-Z8, Thor labs). The stage is controlled via customized LabVIEW software using DC Servo Motor Controllers (KDC101, Thor labs). The sample is illuminated from below by an LED (MCWHL5, LEDD1B, Thorlabs) and imaged by a CCD camera (DCC1545M, Thorlabs).
5
Acoustically-evoked Behavior of Larval Zebrafish
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Larvae were acclimated to constant illumination for at least 3 hours prior to testing, then transferred to the array of 36 individual 9mm diameter testing arenas used for acoustically-evoked behavior, lit obliquely above with a dimmable white LED (MCWHL5, Thor Labs). Behavior was recorded at 500 fps, triggered to start recording 30ms before each "Dark Flash" where the white LED was turned off for 2 seconds. All components were surrounded by a custom black vinyl enclosure to shield from external lights. 6 dpf larvae were presented with 6x “pre-habituation” dark flashes at 150s ISI, followed immediately by 42 “habituating” dark flashes at 15s ISI. Testing took place between the hours of 10:00am and 5:00pm. Videos were tracked and analyzed for response rates and response latency using Flote v2.1 65, and subsequent bout classification was performed through an offline bout-based tracking approach (see “Bout-based Tracking ” section below).
6
High-resolution Imaging of Neuronal Arbors
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Spatial light interference microscopy (SLIM) is an optical microscopy technique that can capture the evolution of living neurons34 (link). Neurons are particularly challenging to image, as complex phenotypes such as arborization are adversely modulated by phototoxicity. A higher resolution SLIM imaging method could decouples amplitude artifacts from high detailed cellular information. When imaging neural networks, we attempt to ameliorate phototoxicity concerns by reducing the illumination intensity (Thorlabs MCWHL5, 30 milliamps, 3% of total power) and average over several images following the hybrid denoising scheme in36 (link). To boost the sensitivity of our measurements, we choose to use Spatial Light Interference Microscopy (SLIM Pro, Phi Optics) which is particularly well suited to imaging the fine details found in neuronal arbors35 (link).
7
Laparoscopic NIR Fluorescence Imaging
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