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Bio plex 6

Manufactured by Bio-Rad
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The Bio-Plex 6.1 software is a data analysis tool designed to work with Bio-Rad's multiplex assay systems. It provides the functionality to acquire, analyze, and manage data generated from these assays.

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Lab products found in correlation

Spectramax plus plate reader, by Molecular Devices (2 mentions) Bio plex 200 system, by Bio-Rad (2 mentions) Bio plex 200 instrument, by Bio-Rad (2 mentions) Mouse duoset elisa, by R&D Systems (1 mentions) Mp5 20f3 capture antibody, by BD (1 mentions) Biotinylated mp5 32c11 detection antibody, by BD (1 mentions) Bio plex pro human th17 cytokine assay, by Bio-Rad (1 mentions) Luminex 100, by DiaSorin (1 mentions) Milliplex map human cytokine chemokine magnetic bead panel, by Merck Group (1 mentions) Xe 5000, by Sysmex (1 mentions) Cobas 6000 analyzer, by Roche (1 mentions)

7 protocols using bio plex 6

1

Multiplex Cytokine Quantification in Mice

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Chemokines and cytokines were quantified by a Cytokines Mouse Magnetic 20-Plex Panel for Luminex (Life Technologies), and a ProcartaPlex mouse IFN-α/IFN-β panel (eBioscience) according to the manufacturer's instructions and data were acquired using a Bio-Plex 200 system (Bio-Rad Laboratories, UK). The concentration of cytokines in each sample was determined according to the standard curve using the Bio-Plex 6 software (Bio-Rad Laboratories). The concentration of CXCL10, CCL2, and TNF-α was additionally measured using mouse DuoSet ELISA (R&D) according to the manufacturer's instructions. IL-6 was detected by ELISA using MP5-20F3 capture antibody and biotinylated MP5-32C11 detection antibody (both from BD Pharmingen). Data were acquired on a SpectraMax Plus plate reader (Molecular Devices) and analysed using SoftMax software (version 5.2).
Makris S., Bajorek M., Culley F.J., Goritzka M, & Johansson C. (2016). Alveolar Macrophages Can Control Respiratory Syncytial Virus Infection in the Absence of Type I Interferons. Journal of Innate Immunity, 8(5), 452-463.
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2

Luminex-based Cytokine Quantification

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Chemokines and cytokines were quantified by a 20-plex Luminex kit according to the manufacturer's instructions (Life Technologies), and data were acquired with a Bio-plex 200 system (Bio-Rad laboratories, United Kingdom). The concentration of cytokines in each sample was determined according to the standard curve using the Bio-plex 6 software (Bio-Rad laboratories). IFN-λ2/3 (R&D), CXCL1 (R&D), or IFN-α (21 (link)) levels in the BAL fluid were measured by enzyme-linked immunosorbent assay (ELISA). Data were acquired on a SpectraMax Plus plate reader (Molecular Devices) and analyzed using SoftMax software (version 5.2).
Goritzka M., Durant L.R., Pereira C., Salek-Ardakani S., Openshaw P.J, & Johansson C. (2014). Alpha/Beta Interferon Receptor Signaling Amplifies Early Proinflammatory Cytokine Production in the Lung during Respiratory Syncytial Virus Infection. Journal of Virology, 88(11), 6128-6136.
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3

Multiplex Cytokine Profiling in Plasma

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Cytokine production in plasma samples was measured using a 17-plex cytokine assay (BioPlex Multiplex human cytokine 17-plex panel; Bio-Rad, Hercules, CA, USA) containing the following analytics: IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-12 (p70), IL-13, IL-17, granulocyte colony stimulating factor, granulocyte macrophage colony stimulating factor, interferon gamma, MCP-1 (also known as CCL2), MIP-1β (also known as CCL4) and TNF-α. The analysis was performed according to the manufacturer’s instructions. A Bio-Plex 200 instrument (Bio-Rad) and its software (Bio-Plex 6.1) were used for analysis.
Wolf-Grosse S., Rokstad A.M., Ali S., Lambris J.D., Mollnes T.E., Nilsen A.M, & Stenvik J. (2017). Iron oxide nanoparticles induce cytokine secretion in a complement-dependent manner in a human whole blood model. International Journal of Nanomedicine, 12, 3927-3940.
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4

Cytokine Profiling via Luminex Assay

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Cell-culture supernatants were analyzed for the presence of IL-17A, IFN-g, and TNF-a by using Bio-Plex Pro Human Th17 Cytokine Assays (Bio-Rad Laboratories), according to the manufacturer's instructions. Cytokine concentrations were measured with a Luminex 100 (Luminex, Austin, Tex) and analyzed by using the software Bio-Plex 6.1 (Bio-Rad Laboratories). The lower level of detection was 1.9 pg/mL for IL-17A, 11.5 pg/mL for IFN-g, and 2.2 pg/mL for TNF-a.
Urbano P.C.M., Aguirre-Gamboa R., Ashikov A., van Heeswijk B., Krippner-Heidenreich A., Tijssen H., Li Y., Azevedo V.F., Smits L.J.T., Hoentjen F., Joosten I, & Koenen H.J.P.M. (2018). TNF-α-induced protein 3 (TNFAIP3)/A20 acts as a master switch in TNF-α blockade-driven IL-17A expression. The Journal of allergy and clinical immunology, 142(2).
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5

Multiplex Cytokine Analysis of Serum Samples

Cited 2 times
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Whole blood was collected by venipuncture at the enrollment visit. Blood for cytokine analysis was collected into serum separator tubes and centrifuged and aliquoted into cryovials following a standard protocol during or immediately following the study visit. Serum aliquots were frozen and stored at -80°C pending shipment to the biorepository and subsequently to the analyzing laboratory. As freeze-thaw cycles can alter cytokine measures, samples in our analysis were not freeze-thawed[18 (link)]. Total numbers of eosinophils and neutrophils were determined by the product of the differential and total cell count. Using 25 μL of participant serum, cytokines were quantified according to the manufacturer’s instructions using a premixed 38 Plex MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore Corporation, Billerica, MA) and analyzed on a Luminex 100/200 analyzer (BioRad, Hercules, CA). Serum cytokine concentrations were determined using Bio-plex 6.1 software (BioRad, Hercules, CA). A similar approach for analyzing cytokines in BAL (bronchoalveolar lavage) fluid from asthmatics has been used previously[19 (link)]. Cytokines included in the analysis are listed in S1 Table.
Brown K.R., Krouse R.Z., Calatroni A., Visness C.M., Sivaprasad U., Kercsmar C.M., Matsui E.C., West J.B., Makhija M.M., Gill M.A., Kim H., Kattan M., Pillai D., Gern J.E., Busse W.W., Togias A., Liu A.H, & Khurana Hershey G.K. (2017). Endotypes of difficult-to-control asthma in inner-city African American children. PLoS ONE, 12(7), e0180778.
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6

Cytokine Profiling of CD8+ T-cells

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Measurement of cytokines present in the supernatants of sorted CD8+CD146+ T-cells and CD8+CD146− T-cells stimulated with plate bound CD3/CD28 for 5 days. This was performed by multiplex bead arrays using kits for Luminex assays (HCYTOMAG-60K-11 Human Cytokine Magnetic Millipore kit, Bellerica, MA) according to the manufacturer’s instructions. Acquisition and analysis was performed on a Luminex-100 instrument using Bio-plex 6.1 software (Bio-rad, Hercules, CA).
Dagur P.K., Biancotto A., Stansky E., Sen H.N., Nussenblatt R.B, & McCoy J.P. (2014). Secretion of Interleukin-17 by CD8+ T Cells Expressing CD146 (MCAM). Clinical immunology (Orlando, Fla.), 152(0), 36-47.
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7

Comprehensive Clinical Biomarker Profiling

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Hemoglobin and hematocrit were measured in EDTA-anticoagulated whole blood using a Sysmex XE-5000. Glucose, cholesterol, triglyceride, HDL, homocysteine, albumin, creatinine, fibrinogen, ferritin, and hsCRP were measured in plasma (Roche Cobas 6000 analyzer, Roche Diagnostics, Indianapolis, IN, USA). HbA1c values were determined by HPLC using a National Glycohemoglobin Standardization Program (NGSP)-certified instrument (D10) made by BioRad Laboratories (Hercules, CA, USA).
TNF-α and MCP-1 were measured in plasma using a Luminex bead array assay with the Multiplex kit (HCYTOMAG-60K-10, Millipore, Chicago, IL, USA) on a Bio plex 200 instrument using the Bio-plex 6.1 software (Bio-Rad, Hercules, CA, USA). Data were available for n = 168 participants. IL-6 was measured in plasma with a digital enzyme-linked immunosorbent assay (ELISA) using SiMOA technology (cat no. 101622, Quantrix, Billerica, MA, USA). IL-6 levels were available in n = 182.
Hormenu T., Shoup E.M., Osei-Tutu N.H., Hobabagabo A.F., DuBose C.W., Mabundo L.S., Chung S.T., Horlyck-Romanovsky M.F, & Sumner A.E. (2020). Stress Measured by Allostatic Load Varies by Reason for Immigration, Age at Immigration, and Number of Children: The Africans in America Study. International Journal of Environmental Research and Public Health, 17(12), 4533.
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