Tsantan Sumtang (catalog # Z20020094) was purchased from Nei Monggol Kaimeng Pharmaceutical Co., Ltd. (Nei Monggol, Hohhot, China). The essential oil content was calculated using steam distillation. Rat N-terminal prohormone brain natriuretic peptide (NT-proBNP) ELISA kits were purchased from Elabscience Biotechnology Co., Ltd. (Hubei, Wuhan, China). Anti-collagen 1 (ab34710), anti-STAT3 (ab76315), anti-Bcl-2 (ab196495), anti-Bax (ab32503), anti-ROCK1 (ab45171), anti-ROCK2 (ab71598), and anti-beta actin (ab8226) were purchased from Abcam (Cambridge, Massachusetts, USA). Anti-cleaved caspase-3 (Asp175) was purchased from Cell Signaling Technology (Boston, USA). Anti-NFATc3 (18222-1-AP) was purchased from Proteintech (Chicago, USA). Proteinase K (Lot No. 1245680100) was purchased from Merck Millipore (Boston, USA). TUNEL kit (in situ cell death detection kit-POD method, Lot No. 10279600) was procured from Roche (Basel, Switzerland). Li Chunhong and phosphotungstic acid (Lot No. 20190115) was purchased from Chengdu Kelong Chemical Reagent Factory (Sichuan, Chengdu, China). Toluidine blue (Lot No. 190118) was purchased from Shanghai Ruji Biotechnology Development Company (China).
Antibeta actin ab8226
Manufactured by Abcam
Sourced in United States
ab8226 is an anti-beta-actin antibody. Beta-actin is a widely expressed cytoskeletal protein that is often used as a loading control in Western blot experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ab185050, by Abcam (2 mentions)
Ab181602, by Abcam (2 mentions)
Bis tris gel, by Thermo Fisher Scientific (2 mentions)
Anti e4bp4 antibody sc 74415, by Santa Cruz Biotechnology (2 mentions)
Anti kdm4c nbp1 49600, by Novus Biologicals (2 mentions)
Anti cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions)
Proteinase k, by Merck Group (1 mentions)
Tunel kit, by Roche (1 mentions)
Gradient polyacrylamide gel, by Bio-Rad (1 mentions)
Peroxidase anti rabbit igg h l, by Vector Laboratories (1 mentions)
Peroxidase anti mouse igg h l, by Vector Laboratories (1 mentions)
Myecl imager, by Thermo Fisher Scientific (1 mentions)
Caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
4 protocols using antibeta actin ab8226
1
Rat Cardiac Fibrosis Biomarker Study
2
Apoptosis Detection by Western Blot
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Apoptosis post exposure was investigated by western blot analysis of cleaved and uncleaved caspase‐3. Cells were lysed in 200 μl 1× Laemmli buffer and boiled at 95°C for 10 min. Samples were stored at −20°C until analysis was performed. Cell lysates and caspase‐3 control extracts (#9663; Cell Signalling Technology) were loaded onto 4%–20% gradient polyacrylamide gels (Bio‐Rad). Proteins were separated by gel electrophoresis, transferred to PVDF membranes (Bio‐Rad) and incubated with appropriate antibodies. Primary antibodies were caspase‐3 antibody (#9662; Cell Signalling Technology) and anti‐beta actin (ab8226; Abcam). Secondary antibodies were peroxidase anti‐mouse IgG (H + L) (PI‐2000; Vector Laboratories) and peroxidase anti‐rabbit IgG (H + L) (PI‐1000; Vector Laboratories). Bands were visualized using the myECL imager (Thermo Fisher Scientific).
3
Western Blot Analysis of Epigenetic Regulators
4
Western Blot Analysis of Epigenetic Regulators
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Twenty-five micrograms of protein from ScAT were suspended in 6.3 μL of sample buffer and 2.5 μL 1 M DTT to run electrophoresis using 10% bis-tris gel (Life Technologies, Rockville, MD, USA). Proteins were transferred to a polyvinylidene difluoride membrane, washed with tris-buffered saline and hybridized with primary anti-EHMT2/G9A antibody ab185050 (Abcam, Cambridge, MA, USA), anti-E4BP4 antibody sc-74415 (Santa Cruz, Dallas, TX, USA), anti-KDM4C NBP1–49600 (Novus Biologicals LLC, Centennial, CO, USA) and normalized to antibeta-actin ab8226 (Abcam, Cambridge, MA, USA) or anti-GAPDH antibody ab181602 (Abcam, Cambridge, MA, USA) 4 °C overnight. The membrane was washed with 1 × PBS containing 0.1% Tween and hybridized with a secondary antibody (goat antimouse 1:10,000 and goat antirabbit 1:10,000) for 1 h in buffer containing 0.1% Tween and 0.01% SDS. Images were developed using an Odyssey Infrared Imager (Li-Cor Biosciences Inc., Lincoln, NE, USA).
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