Alexa flour 488 conjugated anti rabbit antibody

Primary antibodies against ZIKV E protein (#.GTX133314) from Gene Tex, Inc. CA, eIF2α (# 5324), p-eIF2α (# 3388), p-p38 (# 9211),p38 (# 9212), p-JNK(# 9251), JNK (# 9252), IRE1α(# 3294), CHOP (# 2895), Bip (# 3183), HDAC1(# 34589), HERPUD1(# 26730), GADD45 (# 46325), and BCl2 (# 15071) were purchased from Cell Signaling Technologies, MA. Phospho-IRE1α antibody (# ab48187) was obtained from abcam and Actin antibody (# A-5441) was from MilliporeSigma, MA. Rabbit HRP-conjugated secondary antibody (#111-035-144) and mouse HRP-conjugated secondary antibodies (# 715-035-150) were obtained from Jackson ImmunoResearch, PA. Alexa flour 488 conjugated anti-rabbit antibody (# A11008) and Alexa flour 594 conjugated anti-mouse antibody(# A-11032) were obtained from Invitrogen, CA.

Cells (5 × 104 cells/mL) cultured on glass coverslips in six-well plates were fixed with 4% paraformaldehyde and incubated with the primary antibodies in PBS with 1% BSA and 0.1% Triton X-100 at 4 °C overnight. The antibodies used were CD44, β-catenin, E-cadherin, Vimentin, and HSPA1L. Staining was visualized using an Alexa Flour488-conjugated anti-rabbit antibody (Invitrogen). Nuclei were counterstained using 4,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich, St. Louis, MO, USA). Stained cells were visualized via fluorescence microscopy (Olympus BX53F).