Ad vectors were intravenously administered into C57BL/6 mice at a dose of 1 × 1010 IFU/mouse. Two days after administration, the livers were recovered from the mice and homogenized. The luciferase production in the homogenates was measured as previously described.52 (link) For determination of mSEAP levels in the serum, C57BL/6 mice were administered the Ad vectors expressing mSEAP at a dose of 1 × 1010 IFU/mouse. The blood samples were collected on the indicated days via retro-orbital bleeding. mSEAP expression levels were determined using Great EscAPe SEAP Chemiluminescence Kit, version 2.0 (Clontech).
Great escape seap chemiluminescence kit version 2
Manufactured by Takara Bio
The Great EscAPe SEAP Chemiluminescence Kit, version 2.0, is a laboratory tool designed to detect and quantify the activity of secreted alkaline phosphatase (SEAP) in cell culture samples. The kit utilizes a chemiluminescent substrate to enable sensitive and rapid measurement of SEAP levels.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using great escape seap chemiluminescence kit version 2
1
In Vivo Luciferase and mSEAP Assays
2
Assessing Adenoviral Gene Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Neonatal and adult mice were intravenously administered Ad-AHAL2 at a dose of 5.9 × 1011 IFU/kg via retro-orbital sinus. Ad-AHAmSEAP was administered via the tail vein 21 days after the first injection. Two days after Ad-AHAmSEAP injection, blood samples were collected via retro-orbital bleeding and serum samples were prepared as described above. The mSEAP expression levels were determined using a Great EscAPe SEAP Chemiluminescence Kit, version 2.0 (Clontech).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!