Etomoxir hcl

Cells were obtained from the University of Colorado Cancer Center and were authenticated by Single Tandem Repeat analysis (University of Colorado Cancer Center). Cells were passaged in RPMI (except VCaP cells in DMEM) (Invitrogen) containing 5 % fetal bovine Serum supplemented with amino acids and insulin (Hyclone). Human primary prostate-derived cells were isolated (IRB protocol: 00–812) and expanded in keratinocyte medium as previously described [13 (link)]. PC3-LUC cells were from Applied Biological Materials ABM and grown in Prigrow IV media (Applied Biological Materials ABM-TM004) plus 10 % FBS and 1 % P/S. Etomoxir-HCl (Sigma) was dissolved into a 10 mM stock solution in water and stored at −20 °C.

Cell lines (LNCaP and 22Rv1) were obtained from the University of Colorado Cancer Center (UCCC) Tissue Culture Shared Resource (2014) and were authenticated by Single Tandem Repeat analysis. LNCaP MDV-resistant cells were made by growing them in increasing concentrations of MDV ranging from 1.25 to 2.5 uM for 6 months. Cells were passaged in RPMI media containing 5% FBS supplemented with amino acids and Insulin (Hyclone). TRAMPC1 cells (Gift from Dr. Agarwal, 2015, originally from ATCC) were grown in DMEM with 4 mmol/L L-glutamine, 5 μg/mL insulin, 10 nmol/L dihydrotestosterone (DHT), 5% FBS, and 5% Nu Serum (BD Biosciences). Etomoxir-HCl, Ranolazine·2HCL and Perhexiline maleate (all from Sigma-Aldrich, St. Louis, MO) were prepared as a 30, 40 and 15 mM stock solutions, respectively. Enzalutamide (MDV3100, Medivation, Inc), was dissolved as a 20 mM stock in DMSO.