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Insulin humulin r u 100

Manufactured by Eli Lilly
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Insulin (Humulin R U-100) is a laboratory product used for the preparation and analysis of insulin solutions. It is a sterile, concentrated insulin formulation with a concentration of 100 units per milliliter (U-100). This product is intended for use in research and development applications where a precise and consistent insulin solution is required.

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Lab products found in correlation

Lactate assay kit, by Merck Group (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) D glucose, by Merck Group (1 mentions) Medium 199, by Thermo Fisher Scientific (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Ethylene glycol tetraacetic acid (egta), by Thermo Fisher Scientific (1 mentions) Gentamycin, by Thermo Fisher Scientific (1 mentions) Fk228, by Selleck Chemicals (1 mentions) 8 4 chlorophenylthio cpt camp, by Merck Group (1 mentions) Collagen type 4, by Worthington (1 mentions) Contour glucometer, by Bayer (1 mentions) Sterile glucose, by Merck Group (1 mentions)

Spelling variants of this product

Humulin R (427 citations) Humulin (192 citations) Insulin (185 citations) Humulin R insulin (40 citations) Humulin R U-100 (24 citations) Humulin U-100 (8 citations) Insulin (Humulin) (7 citations) Humulin®-R-100 (5 citations)

4 protocols using insulin humulin r u 100

1

Angiotensin II Metabolism and Glucose Uptake

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Angiotensin II (ANG II) acetate was from Bachem (Torrance, CA, USA). Probumin bovine serum albumin fatty acid-free was from EMD Millipore (Billerica, MA, USA). PerkinElmer was the source for [9,3-3H]oleate and [U-14C]glucose. Insulin (Humulin R U-100) was from Eli Lilly and Company (Indianapolis, IN, USA). The lactate assay kit (MAK064) was from Sigma-Aldrich (St. Louis, MO, USA).
Altara R., Harmancey R., Didion S.P., Booz G.W, & Zouein F.A. (2016). Cardiac STAT3 Deficiency Impairs Contractility and Metabolic Homeostasis in Hypertension. Frontiers in Pharmacology, 7, 436.
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2

Characterization of Hepatic Transcriptional Regulation

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Ketamine is from KetaSet® and Xylazine from AnaSed®. Medium 199, HBSS, EGTA, HEPES, PenStrep and Gentamycin are from Life Technology. Collagen type 4 is pursached from Worthington. Insulin (Humulin® R U-100) was purchased from Eli Lilly. 8-(4-chlorophenylthio) (CPT)-cAMP, dexamethasone, cycloheximide, Bovine Serum Albumin (BSA), D-glucose and sodium pyruvate were from Sigma-Aldrich. Lipofectamine2000 and Stealth RNAi™ siRNA against Sin3a were from Thermo Fisher. Trichostatin A (TSA), TMP269 and FK228 were from Selleckchem. TC-H 106 was from Cayman chemical. Insulin was diluted in sterile water. 8-(4-chlorophenylthio) (CPT)-cAMP, D-glucose, sodium pyruvate were dissolved in sterile water. dexamethasone and cycloheximide were dissolved in ethanol (100%). TSA, TMP195, FK228, TC-H 106 were dissolved in DMSO. Anti-FOXO1 (for Western Blot and co-immunoprecipitation, C29H4), anti-SIN3A (for Western Blot, D1B7), and anti-HNF4A (for Western Blot, C11F12) antibodies were from Cell Signaling. Anti-HNF4A ChIP Grade (ab41898), anti-FOXO1A ChIP Grade (ab39670), anti-SIN3A ChIP Grade (ab3479), anti-H3 (ab1791), anti-Ac H3 (ab47915), anti-actin (ab8227) and control IgG antibodies were from Abcam. GCK antibody was from Dr. Magnuson (Vanderbilt University School of Medicine, Nashville, TN).
Langlet F., Haeusler R.A., Lindén D., Ericson E., Norris T., Johansson A., Cook J.R., Aizawa K., Wang L., Buettner C, & Accili D. (2017). Selective Inhibition of FOXO1 Activator/Repressor Balance Modulates Hepatic Glucose Handling. Cell, 171(4), 824-835.e18.
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3

Biochemical Plasma Analysis Protocol

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Unless otherwise indicated, chemicals and enzymes were purchased from Sigma Chemical
Co. (USA). Radiochemicals were obtained from NEN Life Sciences Products, Inc. (USA).
Humulin® R U-100 insulin was obtained from Eli Lilly and Company
(USA). Assay kits for measurements of biochemical plasma parameters were purchased
from Laborclin (Brazil).
Fischer S.V., Appel M.H., Naliwaiko K., Pagliosa D.D., Araújo D.N., Capote A.E., Oliveira B.A, & Fernandes L.C. (2022). Early introduction of exercise prevents insulin resistance in postnatal overfed rats. Brazilian Journal of Medical and Biological Research, 55, e11987.
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4

Glucose Tolerance and Insulin Sensitivity

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After a 4-hour fast, baseline blood glucose levels were measured using a Contour glucometer (Bayer) by cutting the tip of the tail. Mice were given an intraperitoneal (IP) injection of 10% sterile glucose (Sigma-Aldrich, St. Louis, MO) equivalent to 1 g of glucose per kilogram body weight or an IP injection of 1.5 U Humulin R U-100 insulin (Eli Lilly and Company, Indianapolis, IN) per kilogram body weight in 0.9% sterile saline. Blood glucose levels were measured at 15, 30, 45, 60, and 90 min postinjection. During the GTT, plasma was collected for determination of insulin at baseline, 15 and 60 minutes postinjection.
Costford S.R., Tattoli I., Duan F.T., Volchuk A., Klip A., Philpott D.J., Woo M, & Girardin S.E. (2018). Male Mice Lacking NLRX1 Are Partially Protected From High-Fat Diet–Induced Hyperglycemia. Journal of the Endocrine Society, 2(4), 336-347.
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