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Sea957mu

Manufactured by Cloud-Clone
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Sourced in United Kingdom

The SEA957Mu is a laboratory equipment product. It serves the core function of measuring and analyzing samples.

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Lab products found in correlation

Sea471mu, by Cloud-Clone (1 mentions) Mop00, by R&D Systems (1 mentions) Mtr00, by R&D Systems (1 mentions) Ac 06f1, by Immunodiagnostic Systems (1 mentions)

3 protocols using sea957mu

1

Serum Biomarker Analysis in Mice

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Blood samples were collected from the mice, and the serum was centrifuged at 3500 rpm at 4°C for 20 min. The separated sera were analyzed for detection of biochemical markers, including C-terminal telopeptide fragment of type I collagen (CTX1), N-terminal propeptide of type I procollagen (P1NP), osteocalcin (OC), and alkaline phosphatase (ALP) [14 (link)]. CTX1 was measured by a specific enzyme-linked immunosorbent assay (ELISA) kit (CEA665Mu; Cloud-Clone Corp., Katy, TX, USA). P1NP was measured by an ELISA kit for mouse P1NP (SEA957Mu; Cloud-Clone Corp.) according to the manufacturer's instructions. OC was analyzed using a standard commercial ELISA kit for OC (SEA471Mu; Cloud-Clone Corp.). ALP was determined using an autoanalyzer (7020; Hitachi, Tokyo, Japan).
Song L., Bi Y.N., Zhang P.Y., Yuan X.M., Liu Y., Zhang Y., Huang J.Y, & Zhou K. (2017). Optimization of the Time Window of Interest in Ovariectomized Imprinting Control Region Mice for Antiosteoporosis Research. BioMed Research International, 2017, 8417814.
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2

Quantification of Bone Turnover Biomarkers

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For the determination of bone turnover biomarkers, serum of the sacrificed mice was collected immediately post mortem. Quantification was performed using antibody-based detection kits according to the manufacturer’s instructions (CTX-1: AC-06F1, Immunodiagnostic Systems, UK; PINP: SEA957Mu, Cloud Clone Corp., Katy, TX, USA).
Brylka L., Jähn-Rickert K., Baranowsky A., Neven M., Horn M., Yorgan T., Wikman H., Werner S., Lübke A., Amling M., Busse B., Pantel K, & Schinke T. (2022). Spine Metastases in Immunocompromised Mice after Intracardiac Injection of MDA-MB-231-SCP2 Breast Cancer Cells. Cancers, 14(3), 556.
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3

Serum Biomarker Measurement Protocol

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Serum concentrations were measured using commercially available ELISAs for PINP (SEA957Mu, Cloud Clone Corp.), CTX (AC-06F1, Immunodiagnostic Systems), RANKL (MTR00, R&D Systems) and OPG (#MOP00, R&D Systems).
Liao N., Koehne T., Tuckermann J., Triviai I., Amling M., David J.P., Schinke T, & Luther J. (2021). Osteoblast-specific inactivation of p53 results in locally increased bone formation. PLoS ONE, 16(11), e0249894.
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