Paclitaxel

Manufactured by Maclin Biochemical Technology

Sourced in China

Paclitaxel is a natural compound derived from the bark of the Pacific yew tree. It is a widely used pharmaceutical agent that serves as an active ingredient in the production of various anti-cancer medications.

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Lab products found in correlation

8 protocols using paclitaxel

Paclitaxel-Induced Peripheral Neuropathy in Rats

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The rat PIPN model was established according to methods previously described [11 (link), 24 (link), 25 (link)]. Briefly, 6 mg/mL of pharmaceutical grade paclitaxel (Hospira Australia Pty. Ltd., Australia) was diluted with sterile 0.9% saline to 1 mg/mL and given at a dosage of 2 mg/kg intraperitoneally (i.p.) in a volume of 0.5 ml/250 g rat every other day for a total of four injections (days 1, 3, 5, and 7). Control animals received the same volume of sterile saline treatment.
For the study to investigate the vehicle effect of paclitaxel formulation, rats received an equivalent volume of the vehicle for paclitaxel, with proportional amounts of Cremophor EL (C875008, Macklin Inc., Shanghai, China) and 95% dehydrated ethanol diluted in sterile 0.9% saline (Cremophor EL/ethanol 1:1). Control rats received equal amount of saline injection (0.5 ml/250 g). Rats were observed carefully for any abnormal behavioral changes every day following the treatment.

Li Y., Yin C., Liu B., Nie H., Wang J., Zeng D., Chen R., He X., Fang J., Du J., Liang Y., Jiang Y., Fang J, & Liu B. (2021). Transcriptome profiling of long noncoding RNAs and mRNAs in spinal cord of a rat model of paclitaxel-induced peripheral neuropathy identifies potential mechanisms mediating neuroinflammation and pain. Journal of Neuroinflammation, 18, 48.

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Antimicrobial Activity of Functionalized Albumin

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Bovine serum albumin (BSA), polyoxyethylene (10) cetyl ether (Brij 58), rhodamine, Thioflavin T (ThT), rapamycin, thiazolyl blue tetrazolium bromide, and paraformaldehyde were purchased from Sigma-Aldrich. SA was purchased from Aladdin. Paclitaxel was purchased from Macklin. Phosphate buffered saline (PBS), Mueller–Hinton broth (MHB), Mueller–Hinton agar (MHA), and methyl blue (MB) were purchased from Solarbio. Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) was purchased from TCI. Dimethyl sulfoxide (DMSO), ethanol, acetonitrile, 30% hydrogen peroxide (H₂O₂), sodium hydroxide (NaOH), sulfuric acid (H₂SO₄), and Tween 80 were purchased from Sinopharm Chemical Reagent Co., Ltd. 5(6)-aminofluorescein-labeled SA was purchased from Ruixi Biological Technology Co., Ltd. Staphylococcus aureus (S. aureus) (ATCC6538) and Escherichia coli (E. coli) (ATCC8739) were obtained from American Type Culture Collection (USA). The silicone urinary catheter was purchased from Wellead Medical Instruments Co., Ltd. (Guangzhou, China).

Tian J., Fu D., Liu Y., Guan Y., Miao S., Xue Y., Chen K., Huang S., Zhang Y., Xue L., Chong T, & Yang P. (2023). Rectifying disorder of extracellular matrix to suppress urethral stricture by protein nanofilm-controlled drug delivery from urinary catheter. Nature Communications, 14, 2816.

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Cancer Cell Viability Assay

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MDA-MB-468 cells were seeded into 96-well plates at 8000 cells per well and incubated overnight. After 48 h of transient transfection according to the above method, the cells were treated with different concentrations of epirubicin (Macklin, China) for 48 h, or the cells were treated with different concentrations of paclitaxel (Macklin, China) for 36 h and then were assessed for viability using a CCK-8 reagent (APExBIO, USA) as per the manufacturer’s manual. Briefly, 10 μL of the CCK-8 reagent was added to each well of the 96-well plates, which were incubated for 2 h at 37°C. The optical density (OD) value at 450 nm was measured using a SpectraMax Absorbance Reader (Molecular Devices, CMax Plus, USA).

Du Y., Han Y., Wang X., Wang H., Qu Y., Guo K., Ma W, & Fu L. (2022). Identification of Immune-Related Breast Cancer Chemotherapy Resistance Genes via Bioinformatics Approaches. Frontiers in Oncology, 12, 772723.

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Pharmacological Interventions for Neuropathic Pain

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Indomethacin, meloxicam, pregabalin, and paclitaxel were acquired from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). λ-Carrageenan (the standard proinflammatory substance) [32 (link)] and sodium carboxymethyl cellulose were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Pregabalin was dissolved in saline, while indomethacin and meloxicam were suspended in a solution of 0.5% (w/v) sodium carboxymethyl cellulose. The drug was administered intragastrically (indomethacin and meloxicam) or intraperitoneally (pregabalin) in a volume of 0.1 mL/10 g for mice, with the vehicle group receiving the corresponding solvent (saline or 0.5% sodium carboxymethyl cellulose). All rats were administered via intraperitoneal or intragastric injection at a dose of 0.1 mL/100 g. λ-Carrageenan (2%, w/v) was prepared from normal saline. All solutions were prepared the same day the tests were performed. To identify the locus of the antinociceptive effect mediated by the combination of meloxicam and pregabalin in paclitaxel-induced neuropathic pain, meloxicam (5 μg/20 μL) or pregabalin (20 μg/20 μL) was injected intraplantarly into the surface of the left hind paw of mice.

Ma Y., Liu W., Liang L., Ye J., Huang C., Zhuang T, & Zhang G. (2022). Synergistic Antinociceptive Effects of Indomethacin–Pregabalin and Meloxicam–Pregabalin in Paclitaxel-Induced Neuropathic Pain. Biomedicines, 10(6), 1413.

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Synthesis and Characterization of Paclitaxel-HA Conjugates

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Bovine casein (>98%), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were obtained from Sigma–Aldrich, Chemical Co. (St. Louis, MO, USA). Paclitaxel (>99%) and cis-diammineplatinum dichloride were purchased from Shanghai Macklin Biochemical Technology Co., Ltd. (Shanghai, China). Human malignant melanoma cells (A375) were purchased from Shanghai Institutes for Biological Sciences (Chinese Academy of Sciences). All materials for cell culture were obtained from Sangon Biotech (Shanghai) Co., Ltd., Shanghai, China. The preparation of specific-molecular-weight HA oligosaccharides (10,000 Da) was performed by using the methods mentioned in our previous study [12 (link),16 (link)]. High-molecular-weight HA was hydrolyzed by leech hyaluronidase, and the molecular weight of hydrolyzed HA was determined by high performance size exclusion chromatography. All other reagents were of analytical grade.

Wang M., Zhang Y., Fei Z., Xie D., Zhang H., Du Q, & Jin P. (2022). Hyaluronan Oligosaccharides-Coated Paclitaxel-Casein Nanoparticles with Enhanced Stability and Antitumor Activity. Nutrients, 14(19), 3888.

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Cytotoxicity Evaluation of Nanomaterials

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Paclitaxel, SA, MIF, DXM, pyridine, Na₂CO₃, paraformaldehyde, FITC, DAPI, Triton X‐100, MTT, and D‐luciferin potassium salt were all purchased from Shanghai Macklin Biochemical Co., Ltd. All the solvents, including acetone, m ethanol, polyoxyethylene castor oil, ethanol, and DMSO were obtained from J&K Scientific Ltd, and used without further purification. DMEM, RPMI‐1640, FBS, and penicillin‐streptomycin solution were purchased from Gibco Life Technologies. MCF‐7, MDA‐MB‐231, and 4T1 cells were maintained in our laboratory.

Zhao C., Zhang C., Sun X., Guo Q., Liu J., Liu Y., Hao Y., Feng G., Yang L., Liu H, & Liu J. (2021). Paclitaxel‐based supramolecular hydrogel loaded with mifepristone for the inhibition of breast cancer metastasis. Cancer Science, 113(2), 733-743.

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Paclitaxel and Ce6 for Breast Cancer

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Paclitaxel and Ce6 were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). TPGS₁₀₀₀ was obtained by Energy Chemical Co., Ltd. (Shanghai, China). HA (4000 Da) and α-TOS were purchased from Yuanye Bio-Technology Co., Ltd (Shanghai, China). Roswell Park Memorial Institute 1640 (RPMI-1640) medium, PBS, trypsin–EDTA, and penicillin/streptomycin solution, fetal bovine serum (FBS) were purchased from Zhong Sheng Ao Bang Co., Ltd. (Beijing, China). Other used reagents were all commercially available and used without additional purification.
Human breast cancer MCF-7 cells were obtained from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China). Drug-resistant human breast cancer MCF-7/adr cells were obtained from the Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences (Tianjin, China). MCF-7 and MCF-7/adr cells were cultured in RPMI-1640 medium supplemented with 10% FBS. Both cell lines were maintained in a 37°C humidified incubator with a 5% CO₂ atmosphere. MCF-7 and MCF-7/adr cells used in the experiments were in the exponential growth phase, and regular mycoplasma detection was performed to ensure no contamination.

Ju R., Wu F., Tian Y., Chu J., Peng X, & Wang X. (2023). Dual Sensitization Anti-Resistant Nanoparticles for Treating Refractory Breast Cancers via Apoptosis-Inducing. Drug Design, Development and Therapy, 17, 403-418.

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Multifunctional Nanocomposite Platform for Cancer Therapy

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PEDOT:PSS (Clevios PH 1000) was purchased from Wuhan Zhuojia Technology Co., Ltd. (China). Carbon nanotubes (SWCNTs-COOH, diameter 1–3 nm, and length 1–3 μm) were obtained from XFNANO Materials Tech Co., Ltd. (Jiangsu, China). Sodium carboxymethyl cellulose (CMC) and D (+)-glucose were bought from Aladdin Industrial Co., Ltd. (Shanghai, China). Paclitaxel was purchased from Shanghai Macklin Biochemical Co., Ltd. (China). Resveratrol and Janus green B were bought from Sigma-Aldrich (USA). Fluo-4 AM was obtained from Beyotime Biotechnology Co., Ltd. (China). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum, penicillin and streptomycin for cell culture were obtained from GIBCO (USA). All the other chemicals and solvents of analytical grade were obtained from Sinopharm Chemical Reagent Co., Ltd. (China) and used as received unless stated otherwise.

Jiang H., Qi Y.T., Wu W.T., Wen M.Y., Liu Y.L, & Huang W.H. (2020). Intracellular monitoring of NADH release from mitochondria using a single functionalized nanowire electrode. Chemical Science, 11(33), 8771-8778.

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