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7 protocols using tianeptine

1

Adolescent Rat Pharmacology Intervention

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Tianeptine and haloperidol (Sigma Aldrich, St. Louis, MO, USA; Merck KGaA, Darmstadt, Germany) were dissolved in 0.9% NaCl. Separate groups of adolescent rats were treated with Tianeptine (10 mg/kg) or haloperidol (1.5 mg/kg)34 (link),35) (link) once daily for 21 consecutive days (postnatal day 35 to 56).10) (link) The duration of experimental manipulation used for rats (P35–P49) was selected because it roughly approximated mid-adolescence in human.36) (link) Each drug treatment was performed at 10 a.m. every day.
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2

Tianeptine Modulates LPS-Induced Responses

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In all presented experiments, cells were pretreated for 30 min with various concentrations of tianeptine (0.1, 1.0, 10.0, or 50 μM; Sigma-Aldrich, St. Louis, MO, USA), and were then stimulated with lipopolysaccharide (LPS; 100 ng/mL; Escherichia coli 0111:B4, Sigma-Aldrich, St. Louis, MO, USA). Control (unstimulated) cells were treated with vehicle (phosphate-buffered saline (PBS) buffer).
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3

Antidepressant Drugs in Stress Response

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After the behavioral verification, the control and prenatally stressed offspring were divided into six experimental groups (CONTROL + VEH, CONTROL + IMI, CONTROL + TIA, STRESS + VEH, STRESS + IMI, STRESS + TIA; six animals per group) and were treated for 21 days with antidepressant drugs or vehicle. Imipramine (used as a reference drug to compare tianeptine efficiency in behavioral tests; Sigma-Aldrich, St. Louis, MO, USA), tianeptine (Servier, France) or vehicle (0.9 % saline, Polpharma, Poland) was injected intraperitoneally, once per day between 9:00 am and 10:00 am. Imipramine and tianeptine were injected at a dose of 10 mg/ml/kg in a volume of 1 ml/kg. Twenty-four hours after the last injection, the animals were sacrificed by rapid decapitation. The hippocampi and the frontal cortices were dissected.
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4

Pharmacological Reference Compounds Acquisition

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Psychiatric drug reference compounds were purchased from the following sources at the indicated purity. Haloperidol (Sigma-Aldrich, NA), Aripiprazole (Sigma-Aldrich, >98%), Clozapine (Tocris, >99%), Olanzapine (Sigma-Aldrich, >98%), Risperidone (Sigma-Aldrich, >98%), Bupropion (Sigma-Aldrich, >98%), (S)-Duloxetine (Sigma-Aldrich, >98%), Excitalopram (Sigma-Aldrich, >98%), Imipramine (Sigma-Aldrich, >98%), Tianeptine (Sigma-Aldrich, >98%), Buspirone (Sigma-Aldrich, NA), Fenobam (Sigma-Aldrich, >98%), Alprazolam (Sigma-Aldrich, NA), Diazepam (Sigma-Aldrich, NA).
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5

Pharmacological Reference Compounds Acquisition

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Psychiatric drug reference compounds were purchased from the following sources at the indicated purity. Haloperidol (Sigma-Aldrich, NA), Aripiprazole (Sigma-Aldrich, >98%), Clozapine (Tocris, >99%), Olanzapine (Sigma-Aldrich, >98%), Risperidone (Sigma-Aldrich, >98%), Bupropion (Sigma-Aldrich, >98%), (S)-Duloxetine (Sigma-Aldrich, >98%), Excitalopram (Sigma-Aldrich, >98%), Imipramine (Sigma-Aldrich, >98%), Tianeptine (Sigma-Aldrich, >98%), Buspirone (Sigma-Aldrich, NA), Fenobam (Sigma-Aldrich, >98%), Alprazolam (Sigma-Aldrich, NA), Diazepam (Sigma-Aldrich, NA).
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6

Tianeptine Administration in Stress Model

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Tianeptine (Sigma-Aldrich, Seoul, Korea) was dissolved in 0.9% NaCl and injected intraperitoneally (10 mg/kg/daily once) for 24 days. Each drug treatment was performed every day at 10 a.m. during stress procedure.
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7

Culturing SH-SY5Y Cells with Tianeptine

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SH-SY5Y, a human neuroblastoma cell line, was cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco BRL) containing 10% FBS (Gibco BRL). DMEM culture solution was changed every 48 hours until cell maturation. For experimental treatment, tianeptine (Sigma-Aldrich) was prepared at 10 or 20 µM before addition to cell cultures.
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