Tumor xenografts were performed in the rostral midline dorsum using methodology described above (n = 10 per group). A peninsular cranial pedicled dorsal flap was created by making a full thickness U-shaped incision (1.25 cm in width and 2.5 cm in length) on the dorsum just caudal to the xenograft tumor. The flap was elevated and a 0.13mm thick silicone sheet (Invotec International Inc, Jacksonville, FL) was placed below the tissue over the underlying muscle bed. This silicone sheet prevents revascularization from the underlying tissue bed and forces revascularization from the cranial axial blood supply. The flap was closed with simple interrupted stitches using 6–0 nylon suture (Ethilon Nylon Suture; Ethicon Inc., Somerville, NJ). Digital photographs were taken on day 0 and day 10. The degree of ischemia, calculated as the area of ischemia divided by the total flap area, was determined using Adobe Photoshop.
Ethilon nylon suture
Manufactured by Johnson & Johnson
Sourced in United States
Ethilon Nylon Suture is a sterile, non-absorbable surgical suture material made from nylon. It is designed for use in a variety of surgical procedures.
Automatically generated - may contain errors
8 protocols using ethilon nylon suture
1
Tumor Xenograft Flap Revascularization
2
Preparation of Nylon Monofilament Suture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The suture was prepared from a 5 cm-long part of a sterile 4/0 nylon monofilament (Ethilon Nylon Suture, Ethicon Inc. Germany). One end of the suture was rounded carefully by melting with a portable electrocautery unit (Harvard apparatus Ltd, Germany). The end of the suture was therefore kept inside the electrocautery ring for several seconds. Tip diameter was standardized to 0,38-0,40 mm using a micro forge (Narishige MF 900, Japan). To obtain intraoperative control on the length of the intraarterially introduced monofilament, we have marked the proximal 20 mm of the suture with sterile permanent marker in 5 mm distances (Figure 9 A,B). To increase the adhesive properties of the nylon suture, it was coated with Poly-L–Ornithine (PLO, Sigma Aldrich, Germany) by immersing in 1%-PLO solution overnight at room temperature as described previously [1 (link)].
3
Chicken Embryo OFT Banding Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All works in this study were Schedule 1 procedures; they were ethically reviewed at the University of Nottingham and all procedures and facilities are compliant with local and institutional guidelines. Gallus fertilised eggs (Henry Stewart & Co., UK), of the White Leghorn variety, were placed at 38 °C in a humidified rotating incubator. At HH21, the eggs were fenestrated. The OFT‐banded embryos had their OFT constricted with a transverse, double‐overhang knot as previously published (Sedmera et al. 1999 ), using an Ethilon® Nylon Suture (Ethicon; W1770), which had a 3/8 circle needle attached to a 10‐0 suture. The ‘shams’ had the suture passed below the OFT but no ligature was made. Any embryos that showed haemorrhage during the banding procedure or had phenotypic malformations were excluded from the studies. The banding procedure was carried out using a Stemi SV 11 stereomicroscope (Carl Zeiss). All eggs were sealed and reincubated until HH26‐35 without rotation. Incubation times as well as the staging criteria were according to Hamburger‐Hamilton stages (Hamburger & Hamilton, 1992 ).
4
Skin Incision and Closure Procedure
5
Stereotactic Viral Injection in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult mice were anaesthetized by inhalation of 2% isofluorane delivered with a constant air flow (0.4 l min−1). Ketoprofen (5 mg kg−1) and dexamethasone (0.5 mg kg−1) were administered subcutaneously as preemptive analgesia and to prevent brain oedema, respectively, before surgery, and lidocaine (2–4 mg kg−1) was applied intra-incisionally. Mice were mounted in a stereotaxic headframe (Kopf Instruments, 940 series or Leica Biosystems, Angle Two). Stereotactic coordinates were identified (Supplementary Table 5 ). An incision was made over the scalp, a small burr hole drilled in the skull and brain surface exposed. Injections were performed according to the strategies delineated in Supplementary Table 5 . A pulled glass pipette tip of 20–30 μm containing the viral suspension was lowered into the brain; a 300–400 nl volume was delivered at a rate of 30 nl min−1 using a Picospritzer (General Valve Corp); the pipette remained in place for 10 min preventing backflow, prior to retraction, after which the incision was closed with 5/0 nylon suture thread (Ethilon Nylon Suture, Ethicon) or Tissueglue (3M Vetbond), and mice were kept warm on a heating pad until complete recovery.
6
Bilateral Lower Extremity Amputation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice underwent bilateral lower extremity amputation at the hip prior to testing, to examine the injury pattern when the effects of lower extremity flail are eliminated. Amputation was performed with a posterior approach to the hip joint, disarticulation of the hip, removal of the lower limb and subsequent closure with 4.0 nylon sutures (Ethilon Nylon Suture, Ethicon, New Jersey, USA).
7
Calvarial Defect Repair in Rat Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After general anesthetization with a mixture of ketamine 35 mg/kg and xylazine 5 mg/kg, the dorsal part of the scalp covering the calvarias was shaved and disinfected with iodophor gauze. A dermoperiosteal incision was made along the midline of the cranium from the frontal bone to the occipital bone. A full-thickness flap was elevated to expose the surgical area. Two 10-mm-diameter calvarial defects were created on both sides of the midline using a trephine bur under profuse saline irrigation. The resected bone blocks were carefully removed, and the defects were treated as described in Section 4.3.1 . Then, the periosteum and scalp skin were sutured in layers with polylactic acid sutures (Vycril 5.0, Ethicon, Raritan, NJ, USA) and nylon monofilament sutures (ETHILON® Nylon Suture, Ethicon, Raritan, NJ, USA). The animals were intramuscularly injected with antibiotics two times daily for 3 days.
8
Orthotopic Implantation of GL261-luc Cells in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Under isoflurane anesthesia, a 0.5-cm longitudinal incision was made on the scalp, and a burr hole was drilled using a high-speed dental drill (ML: ±1.5; AP: +1.5). Using a stereotactic frame, the needle of a Hamilton syringe was then lowered 3.5 mm into the striatum and a total of 4–6 × 104 GL261-luc cells in 1–2 μL were injected, as previously described (Ayasoufi et al., 2020 (link)). The wound was closed using 6–0 ETHILON® Nylon Suture (Ethicon, #1660G).
To assess tumor burden in GL261-luc-bearing mice, bioluminescence imaging was used as previously described (Ayasoufi et al., 2020 (link)). Mice were intraperitoneally injected with 200 μL of 15 mg/μL D-Luciferin in PBS (Goldbio, #LUCK-1G), and anaesthetized with 2% isoflurane during imaging. Mice were scanned using the IVIS Spectrum system (Xenogen Corp.) at Mayo Clinic, running Living Image software.
To assess tumor burden in GL261-luc-bearing mice, bioluminescence imaging was used as previously described (Ayasoufi et al., 2020 (link)). Mice were intraperitoneally injected with 200 μL of 15 mg/μL D-Luciferin in PBS (Goldbio, #LUCK-1G), and anaesthetized with 2% isoflurane during imaging. Mice were scanned using the IVIS Spectrum system (Xenogen Corp.) at Mayo Clinic, running Living Image software.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!