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Cd8 bv650 rpa t8

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CD8-BV650 is a fluorochrome-conjugated antibody that specifically binds to the CD8 antigen. CD8 is a cell surface glycoprotein that is expressed on a subset of T cells, known as cytotoxic T cells, and some natural killer cells. The BV650 fluorochrome is used for flow cytometry applications.

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Lab products found in correlation

Efluor 506, by Thermo Fisher Scientific (5 mentions) Cd4 apcefluor 780 rpa t4, by Thermo Fisher Scientific (3 mentions) Trizol ls reagent, by Thermo Fisher Scientific (2 mentions) Ccr7 percp cy5, by BioLegend (2 mentions) Anti human cd3 alexa fluor 700, by BD (2 mentions) Cd45ra efluor 450 hi100, by Thermo Fisher Scientific (2 mentions) Facsaria 3 fusion cell sorter, by BD (2 mentions) Cd19 v500, by BD (2 mentions) Lsrii cytometer, by BD (2 mentions) Tigit percp efluor710, by Thermo Fisher Scientific (2 mentions) Vα7.2 pe cy7 3c10, by BioLegend (2 mentions) Anti human cd3 alexa fluor 700 ucht1, by BD (2 mentions) Cd4 allophycocyanin efluor 780 rpa t4, by Thermo Fisher Scientific (2 mentions) Cd161 allophycocyanin hp 3g10, by Thermo Fisher Scientific (2 mentions) Cxcr6 pe dazzle594, by BioLegend (2 mentions) Ccr1 percp cy5.5 5f10b29, by BioLegend (2 mentions) Cxcr4 pe dazzle 594, by BioLegend (2 mentions) Cd127 fitc ebiordr5, by Thermo Fisher Scientific (2 mentions) Cd14 bv421, by BioLegend (2 mentions) Cd14 v500, by BD (2 mentions)

Spelling variants of this product

RPA-T8 (46 citations) CD8 BV650 (13 citations)

5 protocols using cd8 bv650 rpa t8

1

Phenotypic Analysis of Memory CD8 T Cells

Cited 1 time
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Ten million PBMCs were stained with fixable viability dye eFluor 506 (eBioscience) and with anti-human CD3-Alexa Fluor 700 (UCHT1; BD Pharmingen), CD4-APCeFluor 780 (RPA-T4; eBioscience), CD8-BV650 (RPA-T8; BioLegend), CD45RA-eFluor 450 (HI100; eBioscience), and CCR7-PerCP Cy5.5 (UCHL1; BioLegend) as previously described (29 (link)). Briefly, cells were incubated in PBS containing 10% FBS for 10 min at 4°C and then stained with PBS containing the conjugated Abs for 30 min at 4°C. After two washes in PBS, cells were resuspended in PBS, and cell sorting was performed on a BD FACSAria III/Fusion Cell Sorter (Becton Dickinson). A total of 100,000 memory CD8 T cells (see Supplemental Fig. 1A for gating strategy) was sorted into TRIzol LS reagent (Invitrogen).
Pomaznoy M., Kuan R., Lindvall M., Burel J.G., Seumois G., Vijayanand P., Taplitz R., Gilman R.H., Saito M., Lewinsohn D.M., Sette A., Peters B, & Arlehamn C.S. (2020). Quantitative and Qualitative Perturbations of CD8+ MAITs in Healthy Mycobacterium tuberculosis–Infected Individuals. ImmunoHorizons, 4(6), 292-307.
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2

Multiparametric Phenotyping of MAIT Cells

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PBMCs were stained with 1:100 MR1 5-OP-RU or 6-FP (as a control) tetramer for 40 min at room temperature. After 40 min, incubation cells were also stained with fixable viability dye eFluor 506 (eBioscience) and with combinations of anti-human CD3-Alexa Fluor 700 (UCHT1; BD Pharmingen), CD4-allophycocyanin-eFluor 780 (RPA-T4; eBioscience), CD8-BV650 (RPA-T8; BioLegend), Vα7.2-PE-Cy7 (3C10; BioLegend), CD161-allophycocyanin (HP-3G10; eBioscience), TIGIT-PerCP-eFluor710 (MBSA43; eBioscience), CXCR6-PE Dazzle594 (K041E5; BioLegend), CCR1-PerCP Cy5.5 (5F10B29; BioLegend), CD243-BV421 (UIC2; BD Biosciences), CXCR4-PE Dazzle 594 (12G5; BioLegend), CD127-FITC (eBioRDR5; eBioscience), CD14-V500 (M5E2; BD Biosciences), CD14-BV421 (HCD14; BioLegend), and CD19-V500 (HIB19; BD Biosciences) for 30 min at room temperature. After two washes in PBS, cells were resuspended in PBS and cells were acquired on a LSRII Cytometer (Becton Dickinson).
Pomaznoy M., Kuan R., Lindvall M., Burel J.G., Seumois G., Vijayanand P., Taplitz R., Gilman R.H., Saito M., Lewinsohn D.M., Sette A., Peters B, & Arlehamn C.S. (2020). Quantitative and Qualitative Perturbations of CD8+ MAITs in Healthy Mycobacterium tuberculosis–Infected Individuals. ImmunoHorizons, 4(6), 292-307.
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3

Multiparametric Flow Cytometry Analysis of Activated CD4+ T Cells

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PBMCs were thawed and 1 million were added to a round-bottom 96-well plate and stimulated with the respective antigens (peptide pools, Mav and Mtb lysates, PHA and DMSO control). Plates were incubated at 37°C in a humidified CO2 incubator for 20-24 hrs. Plates were centrifuged and cells were resuspended in PBS with 10% (v/v) FBS and incubated at 4°C for 10 min. Cells were then stained with fixable viability dye eFluor506 (eBioscience) and an antibody mixture containing anti-human CD3-AF700 (UCHT1; ThermoFisher), CD4-APCeFluor780 (RPA-T4; eBioscience), CD8-BV650 (RPA-T8; BioLegend), CD14-V500 (M5E2; BD Bioscience), CD19-V500 (HIB19; BD Bioscience), CD25-PerCPCy5.5 (BC96; BioLegend), CD69-PECy7 (FN50; eBioscience), CD137-APC (4B4-1; BioLegend), CD154-FITC (TRAP1; BD Bioscience), OX40-BV421 (Ber-ACT35; BioLegend), and PD-L1-PE (29E.2A3; BioLegend) for 30 min at 4°C. Cells were washed, resuspended in PBS and acquired on a BD LSR II flow cytometer (BD Biosciences). Analysis to compare frequencies of activated CD4+ T cells was completed on FlowJo. The total number of CD4+ T cells expressing combinations of activation markers was determined with background values (as determined from the medium alone control) subtracted. The gating strategy is found in Figure S1A.
Lindestam Arlehamn C.S., Benson B., Kuan R., Dill-McFarland K.A., Peterson G.J., Paul S., Nguyen F.K., Gilman R.H., Saito M., Taplitz R., Arentz M., Goss C.H., Aitken M.L., Horne D.J., Shah J.A., Sette A, & Hawn T.R. (2022). T-cell deficiency and hyperinflammatory monocyte responses associate with Mycobacterium avium complex lung disease. Frontiers in Immunology, 13, 1016038.
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4

Phenotypic Analysis of Memory CD8 T Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Ten million PBMCs were stained with fixable viability dye eFluor 506 (eBioscience) and with anti-human CD3-Alexa Fluor 700 (UCHT1; BD Pharmingen), CD4-APCeFluor 780 (RPA-T4; eBioscience), CD8-BV650 (RPA-T8; BioLegend), CD45RA-eFluor 450 (HI100; eBioscience), and CCR7-PerCP Cy5.5 (UCHL1; BioLegend) as previously described (29 (link)). Briefly, cells were incubated in PBS containing 10% FBS for 10 min at 4°C and then stained with PBS containing the conjugated Abs for 30 min at 4°C. After two washes in PBS, cells were resuspended in PBS, and cell sorting was performed on a BD FACSAria III/Fusion Cell Sorter (Becton Dickinson). A total of 100,000 memory CD8 T cells (see Supplemental Fig. 1A for gating strategy) was sorted into TRIzol LS reagent (Invitrogen).
Pomaznoy M., Kuan R., Lindvall M., Burel J.G., Seumois G., Vijayanand P., Taplitz R., Gilman R.H., Saito M., Lewinsohn D.M., Sette A., Peters B, & Arlehamn C.S. (2020). Quantitative and Qualitative Perturbations of CD8+ MAITs in Healthy Mycobacterium tuberculosis–Infected Individuals. ImmunoHorizons, 4(6), 292-307.
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5

Multiparametric Phenotyping of MAIT Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
PBMCs were stained with 1:100 MR1 5-OP-RU or 6-FP (as a control) tetramer for 40 min at room temperature. After 40 min, incubation cells were also stained with fixable viability dye eFluor 506 (eBioscience) and with combinations of anti-human CD3-Alexa Fluor 700 (UCHT1; BD Pharmingen), CD4-allophycocyanin-eFluor 780 (RPA-T4; eBioscience), CD8-BV650 (RPA-T8; BioLegend), Vα7.2-PE-Cy7 (3C10; BioLegend), CD161-allophycocyanin (HP-3G10; eBioscience), TIGIT-PerCP-eFluor710 (MBSA43; eBioscience), CXCR6-PE Dazzle594 (K041E5; BioLegend), CCR1-PerCP Cy5.5 (5F10B29; BioLegend), CD243-BV421 (UIC2; BD Biosciences), CXCR4-PE Dazzle 594 (12G5; BioLegend), CD127-FITC (eBioRDR5; eBioscience), CD14-V500 (M5E2; BD Biosciences), CD14-BV421 (HCD14; BioLegend), and CD19-V500 (HIB19; BD Biosciences) for 30 min at room temperature. After two washes in PBS, cells were resuspended in PBS and cells were acquired on a LSRII Cytometer (Becton Dickinson).
Pomaznoy M., Kuan R., Lindvall M., Burel J.G., Seumois G., Vijayanand P., Taplitz R., Gilman R.H., Saito M., Lewinsohn D.M., Sette A., Peters B, & Arlehamn C.S. (2020). Quantitative and Qualitative Perturbations of CD8+ MAITs in Healthy Mycobacterium tuberculosis–Infected Individuals. ImmunoHorizons, 4(6), 292-307.
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