Cd81 5a6

To characterise MV flow cytometrically, the following antibodies were used: CD45 (J33, #A07782), CD235a (11E4B-7–6, #A07792, both Beckman Coulter), CD62P (AK4, #304905), CD47 (CC2C6, #323109), MUC1 (16A, #355604) EGFR (AY13, #352904), EMMPRIN (HIM6, #306207), CD62E (HAE-1f, #336008), EpCAM (9C4, #324208, all from Biolegend). To analyse CD63 expression, vesicles were incubated with the unlabelled CD63 antibody (H5C6, #556019, BD) or mouse IgG (#sc-2025, Santa Cruz Biotechnology) and signals visualised using a FITC-labelled secondary antibody (#sc-2010, Santa Cruz Biotechnology). Protein expression by Western Blotting was assessed using primary antibodies against EMMPRIN (H200, #sc-13976), MUC1 (VU4H5, #sc-7313), Tsg101 (C-2, #sc-7964), Hsp90 (F-8, #sc-13119, all from Santa Cruz Biotechnology), HDAC1 (#2062), EGFR, (D38B1, #4267, both from Cell Signaling), CD9 (MEM-61, #21270091, Immunotools), CD81 (5A6, #349501, Biolegend), Wnt5a (442625, #MAB645, R&D systems), syntenin (EPR8102, #ab133267, Abcam), GM130 (35/GM130, #610823, BD Transduction Laboratories) or Tubulin (DM1A, #05–829, Millipore). HRP-labelled secondary antibodies were purchased from Santa Cruz Biotechnology (#sc-2004, #sc-2005, #sc-2006).