Anti bim antibody

American Type Culture Collection (ATCC) supplied all cancer cells and TCMK-1 cells (Manassas, VA, USA), and Korea Cell Line Bank supplied the human skin fibroblasts (HSF). Cells were grown in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum, 5% penicillin–streptomycin, and 100 μg/mL gentamycin. Calbiochem supplied N-acetylcysteine (NAC) and trolox (San Diego, CA, USA), and R&D system supplied z-VAD-fmk, recombinant human recombinant TRAIL, and anti-survivin antibodies (Minneapolis, MN, USA). Santa Cruz Biotechnology supplied anti-Mcl-1, anti-cIAP2, anti-Itch, anti-Cbl, and anti-ATF4 antibodies (Dallas, TX, USA), and Cell Signaling Technology provided anti-PARP-1, anti-Bcl-2, anti-Bcl-xL, anti-caspase-3, anti-cIAP1, anti-DR5, and anti-CHOP antibodies (Beverly, MA, USA). Enzo Life Sciences provided anti-c-FLIP and anti-GRP78/94 antibodies (Ann Arbor, MI, USA), and Abcam supplied the anti-DR4 antibody (Cambridge, MA, USA). BD Biosciences supplied the anti-Bim antibody (San Jose, CA, USA). Sigma Chemical Co. supplied other reagents and the anti-actin antibody (St. Louis, MO, USA).