Histamine dihydrochloride

Cedar pollen extract (PE) and pollen extract-galactomannan conjugate (PE-GM) were purchased from Wako Filter Technology Company (Tokyo, Japan). Fluorescein-4-isothiocyanate (FITC) was purchased from Dojindo (Kumamoto, Japan). Cyclohexane and isopropyl myristate (IPM) were obtained from Wako Pure Chemical Industries (Kyoto, Japan) and Tokyo Chemical Industry (Tokyo, Japan), respectively. A surfactant sucrose laurate (L-195) was kindly provided by Mitsubishi–Kagaku Foods (Tokyo, Japan). RPMI-1640 medium, fetal bovine serum (FBS), antibiotic-antimycotic solution, and Imject Alum were from Thermo Fisher Scientific (Waltham, MA, USA). Histamine dihydrochloride was provided by Nacalai Tesque (Kyoto, Japan). Biotin-conjugated Cry j 1 was obtained from Funakoshi (Tokyo, Japan). Horseradish peroxidase-labeled rabbit anti-mouse IgG 1, and IgG 2a were obtained from Rockland Immunochemicals (Gilbertsville, PA, USA). All other reagents used in the experiments were of analytical grade.

Histamine dihydrochloride was obtained from Nakalai Tesque (Kyoto, Japan). 2-Pyridylethylamine dihydrochloride and 4-methylHistamine dihydrochloride were gifts from Drs. W.A.M. Duncan and G.J. Durant (The Research Institute, Smith Kline & French Laboratories, Welwyn Garden City, Herts). d-Chlorpheniramine maleate and famotidine were obtained from Takeda Pharmaceutical Company (Osaka, Japan) and Yamanouchi Pharmaceuticals (Tokyo), respectively. Compound 48/80 trihydrochloride was obtained from Funakoshi (Tokyo).

R848 was purchased from Enzo life science (Farmingdale, NY). Cyclohexane and Rhodamine‐dioleyl phosphatidylethanolamine (Rho‐DOPE) were purchased from Wako Pure Chemical Industries (Kyoto, Japan) and Avanti Polar Lipids (Alabaster, AL), respectively. Isopropyl myristate (IPM) and 5‐carboxyfluorescein (5‐CF) N‐succinimidyl ester were purchased from Tokyo Chemical Industry (Tokyo, Japan). Cedar Pollen Extract‐Cj was supplied by Cosmo Bio (Tokyo, Japan), and Imject Alum from Thermo Scientific (Waltham, MA). Cry j 1 and Biotin‐conjugated Cry j 1 were obtained from Hayashibara (Okayama, Japan). Histamine dihydrochloride was provided by Nacalai Tesque (Kyoto, Japan). Yucatan micropig skin was purchased from Charles River (Burlington, MA). A surfactant sucrose laurate (L‐195) was kindly supplied by Mitsubishi‐Kagaku Foods (Tokyo, Japan).