A total of 16 male Sprague–Dawley (SD) rats that were six weeks of age and weighed 180-200 g, were included. Rats were randomly divided into 4 groups (4 rats in each group): group I (control diet, saline infused, control water), group II (control diet, porcine pancreatic elastase (PPE) infused, control water), group III (hypercholesterol diet, PPE infused, control water), and group IV (hypercholesterol diet, PPE infused, water mixed with statin) [10 (link), 11 (link)]. The control diet was both low fat and low cholesterol (D12337) (Research Diets, New Brunswick, NJ, USA), while the hypercholesterol diet was a rat chow designed to be high in cholesterol (Paigen’s atherogenic rodent diet, D12336) (Research Diets, New Brunswick, NJ, USA) [10 (link)]. Atorvastatin (Lipitor, Pfizer, NY, USA) (0.2 μg/g body weight/day) was administered by standard oral gavage in group IV for 5 weeks [1 (link)].
D12337
Manufactured by Research Diets
Sourced in United States
D12337 is a laboratory diet formulation produced by Research Diets. It is a standard chow diet designed for use in animal research. The product provides a balanced nutrient profile to support the nutritional needs of laboratory animals.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using d12337
1
Hypercholesterol Diet, Elastase, and Statin Treatment
2
Homozygous MM-1α L110R Mouse Model
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Homozygous MM-1α L110R mice [17] (link) were kindly provided by Dr. Patsy M. Nishina. C57BL/6 and MM-1α L110R mice were fed a normal diet (D12337, Research Diets, Inc. New Brunswick, NJ). Since homozygous MM-1α L110R mice were infertile, heterozygous MM-1α L110R mice were mated, genotypes of their offspring were determined by DNA sequencing of mouse tail DNA, and then homozygous MM-1α L110R mice were obtained. Male mice at 17 weeks of age were used in this study. The nucleotide sequence for a primer used for DNA sequencing is 5′-ATGTACGTCCCCGGGAAGCTACACG-3′.
Establishment of cell lines from wild-type and L110R MM-1α mice was described previously [13] (link). Briefly, primary cells from newborn mice were immortalized with T antigen of simian virus 40 and cultured in Dulbecco׳s modified Eagle׳s medium (DMEM) with 10% calf serum.
Establishment of cell lines from wild-type and L110R MM-1α mice was described previously [13] (link). Briefly, primary cells from newborn mice were immortalized with T antigen of simian virus 40 and cultured in Dulbecco׳s modified Eagle׳s medium (DMEM) with 10% calf serum.
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