PBMCs were incubated with Fc receptor blocking solution (BioLegend) and then stained with mixtures of the following monoclonal antibodies against human surface antigens: anti-CD3-Brilliant Violet 421 (clone UCHT1), anti-CD4-APC/Fire 750 (clone RPA-T4), anti-CD45RA-Brilliant Violet 605 (clone HI100), anti-CD279 (PD1)-PE (clone EH12.2H7), and anti-CD185 (CXCR5)-PE/Dazzle594 (clone J252D4) (all from Biolegend) for T cell subset analysis; anti-CD19-PE/Dazzle594 (clone HIB19), anti-CD20- Alexa Fluor 700 (clone 2H7), anti-CD27-APC/Fire 750 (clone M-T271), anti-IgD-Brilliant Violet 421 (clone IA6-2) (all from Biolegend), and anti-CD38-FITC (clone T16) (Beckman Coulter, Brea, CA, USA) for B cell subset analysis. Cells were washed and stained with 7-AAD Viability Staining Solution (BD Biosciences). Data were acquired on a FACS LSR Fortessa (BD Biosciences) and analyzed using FlowJo software (TreeStar Inc.).
Anti cd38 fitc clone t16
Manufactured by Beckman Coulter
Sourced in United States
The Anti-CD38-FITC (clone T16) is a fluorescently-labeled monoclonal antibody that binds to the CD38 antigen. CD38 is a cell surface glycoprotein expressed on various cell types, including T cells, B cells, and plasma cells. This antibody can be used for the identification and analysis of CD38-positive cells in flow cytometry applications.
Automatically generated - may contain errors
2 protocols using anti cd38 fitc clone t16
1
Comprehensive T and B Cell Profiling
2
Quantifying SARS-CoV-2 Spike RBD-specific Antibodies
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Recombinant biotinylated SARS-CoV-2 spike RBD proteins of the wild type and the Omicron variant (Acrobiosystems) were tetramerized with streptavidin APC and streptavidin BV605 (both from BioLegend) as previously described17 (link),52 (link),57 (link). Two to four million cryopreserved PBMCs were first incubated with Fc receptor blocking solution and then stained with Fluorescent-labeled Spike RBD and mixtures of the following monoclonal antibodies against human surface antigens: anti-CD3- PerCP/Cyanine5.5 (clone UCHT1), anti-CD14-PerCP/Cyanine5.5 (clone M5E2), anti-CD20- Alexa Fluor 700 (clone 2H7), anti-CD27-APC/Fire 750 (clone M-T271), anti-IgM-PE/Cyanine7 (clone MHM-88), and anti-IgG Fc-Brilliant Violet 421 (clone M1310G05) (all from BioLegend); anti-CD8- PerCP/Cyanine5.5 (clone SK1), anti-CD19- V500 (clone HIB19), and anti-IgD-PE-CF594 (clone IA6-2) (all from BD Biosciences); and anti-CD38-FITC (clone T16) (Beckman Coulter). Cells were washed and stained with 7-AAD Viability Staining Solution (BD Biosciences). Data were acquired on a FACS LSR Fortessa (BD Biosciences) and analyzed using FlowJo software (TreeStar Inc.). Data at each time point were obtained by subtracting the frequency before the vaccine.
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