Analytical grade acetone

Manufactured by Avantor

Sourced in France

Analytical grade acetone is a high-purity solvent commonly used in various laboratory applications. It serves as a versatile chemical reagent and cleaning agent, known for its ability to dissolve a wide range of organic compounds.

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4 protocols using analytical grade acetone

Extraction and Analysis of Phenolic Compounds

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Analytical grade acetone, methanol, ethanol and water (VWR International s.r.l., Milan, Italy) were used for the extraction of phenolic and flavonoid compounds. Trolox (6-hydroxy-2,5,7,8-tetramethilchroman-2-carboxylic acid, 97%), DPPH (2,2-Diphenyl-1-picrylhydrazyl, 95%), ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt, potassium persulphate, potassium acetate, aluminium chloride and Folin-Ciocâlteu reagent were obtained from Sigma-Aldrich (Milan, Italy).
Analytical standard grade vanillic acid (97%), ellagic acid (97%), p-coumaric acid (98%), ferulic acid (99%), quercetin (98%), gallic acid (98%) were also purchased from Sigma-Aldrich (Milan, Italy). HPLC gradient grade methanol and water (VWR International s.r.l., Milan, Italy) was used as chromatographic mobile phase. Formic acid (99%; Sigma Aldrich, Milan, Italy) was used to acidify the aqueous mobile phase, prepared with high-purity water. All samples were filtered through a syringe filters in PVDF, pore size 0.45 µm (Millipore, SER.DIA s.r.l., Reggio Calabria, Italy).

Ceramella J., La Torre C., De Luca M., Iacopetta D., Fazio A., Catalano A., Ragno G., Longo P., Sinicropi M.S, & Rosano C. (2022). Exploring the anticancer and antioxidant properties of Vicia faba L. pods extracts, a promising source of nutraceuticals. PeerJ, 10, e13683.

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Analytical Extraction and UHPLC Analysis

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Analytical-grade acetone (VWR International S.A.S., France) was used during extraction.
For oxidation, a pH 10 carbonate buffer (50 mM; sodium carbonate/sodium hydrogen
carbonate, J.T. Baker, Deventer, Netherlands) and, for stopping the oxidation, formic acid
(J.T. Baker, Deventer, Netherlands) were used. For the UHPLC analysis, LC–MS-grade
acetonitrile was purchased from VWR International S.A.S. (USA), and LC–MS-grade
formic acid was purchased from Sigma Aldrich (Seelze, Germany). The Milli-Q water used was
purified with the Millipore Synergy UV (Merck KGaA, Darmstadt, Germany) system.

Imran I.B., Karonen M., Salminen J.P, & Engström M.T. (2021). Modification of Natural Proanthocyanidin Oligomers and Polymers Via Chemical Oxidation under Alkaline Conditions. ACS Omega, 6(7), 4726-4739.

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Solvents for Analytical Procedures

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Technical grade acetone was purchased from VWR (Haasrode, Belgium). Analytical grade acetone, analytical grade methanol, HPLC gradient grade acetonitrile, HPLC gradient grade methanol, HPLC grade phosphoric acid, HPLC grade formic acid and LC-MS grade formic acid were purchased from VWR International (Fontenay-Sous-Bois, Paris, France). HPLC grade n-otanol and LC-MS grade acetonitrile were purchased from Merck (KGaA, Darmstadt, Germany). Aa grade ethanol (≥99.5%) was purchased from Altia OyJ (Helsinki, Finland). Ultra-pure type I water was purified with Merck Millipore Synergy UV system.

Virtanen V, & Karonen M. (2020). Partition Coefficients (logP) of Hydrolysable Tannins. Molecules, 25(16), 3691.

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Liquid Chromatography-Mass Spectrometry Reagents

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LC-MS grade acetonitrile was from Sigma-Aldrich GmbH (Steinheim, Germany) and formic acid (for LC-MS) and analytical grade acetone from VWR (Helsinki, Finland). LC grade acetonitrile was from Lab-Scan (Dublin, Ireland) and phosphoric acid from J.T. Baker (Deventer, Netherlands). Water was purified with either a Millipore Synergy UV (Merck KGaA, Darmstadt, Germany) or an Elgastat UHQ-PS (Elga, Kaarst, Germany) water purification system. Acetone-d6 (99.96 %) was from Euriso-top SAS (St-Aubin Cedex, France).

Suvanto J., Tähtinen P., Valkamaa S., Engström M.T., Karonen M, & Salminen J.P. (2018). Variability in Foliar Ellagitannins of Hippophaë rhamnoides L. and Identification of a New Ellagitannin, Hippophaenin C. Journal of agricultural and food chemistry, 66(3).

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