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Anti ddit4 antibody

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The Anti-DDIT4 antibody is a laboratory reagent used for the detection and analysis of the DDIT4 (DNA-Damage Inducible Transcript 4) protein. DDIT4 is a regulator of the mTOR signaling pathway and plays a role in cellular responses to various stress stimuli. This antibody can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to study the expression and localization of DDIT4 in biological samples.

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Lab products found in correlation

Pvdf membrane, by Merck Group (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Anti atg5 antibody, by Cell Signaling Technology (2 mentions) Clb buffer, by Cell Signaling Technology (2 mentions) Anti ikbα antibody, by Cell Signaling Technology (2 mentions) Anti nf kb p65 antibody, by Cell Signaling Technology (2 mentions) Anti mettl3 antibody 15073 1 ap, by Proteintech (2 mentions) Anti flag hrp, by Cell Signaling Technology (2 mentions)

Close spelling variants of this product

DDIT4 (6 citations) Anti-DDIT4 ( (3 citations) Anti-TM (2 citations)

2 protocols using anti ddit4 antibody

1

Western Blot Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed twice with ice-cold PBS and ruptured with CLB buffer (Cell Signaling Technology) containing PMSF and cocktail inhibitor. Cell lysates were resolved by SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA) and then blotted. Specific antibodies used are listed below: anti-Mettl3 antibody (15073–1-AP, 1:500) was from Proteintech; anti-DDIT4 antibody (1:1000) was from Proteintech; antibody to IkBα phosphorylated at Ser32 (2859S, 1:1000), anti-IkBα antibody (9234S, 1:3000), antibody to P70S6K phosphorylated at T389 (9234S, 1:1000), antibody to AKT phosphorylated at T308 (4056S, 1:1000), antibody to p65 phosphorylated at Ser536 (3031S, 1:1000), anti-NFkB p65 antibody (6956S, 1:1000), anti-ATG5 antibody (12994S, 1:1000), anti-β-actin (3700S, 1:10000), anti-Flag-HRP (2044S, 1:20000) were from Cell Signaling Technology. All of the unprocessed scans of the blots were shown in the Source Data file.
Qin Y., Li B., Arumugam S., Lu Q., Mankash S.M., Li J., Sun B., Li J., Flavell R.A., Li H.B, & Ouyang X. (2021). m6A mRNA methylation-directed myeloid cell activation controls progression of NAFLD and obesity. Cell reports, 37(6), 109968.
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2

Western Blot Analysis of Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed twice with ice-cold PBS and ruptured with CLB buffer (Cell Signaling Technology) containing PMSF and cocktail inhibitor. Cell lysates were resolved by SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, Bedford, MA, USA) and then blotted. Specific antibodies used are listed below: anti-Mettl3 antibody (15073–1-AP, 1:500) was from Proteintech; anti-DDIT4 antibody (1:1000) was from Proteintech; antibody to IkBα phosphorylated at Ser32 (2859S, 1:1000), anti-IkBα antibody (9234S, 1:3000), antibody to P70S6K phosphorylated at T389 (9234S, 1:1000), antibody to AKT phosphorylated at T308 (4056S, 1:1000), antibody to p65 phosphorylated at Ser536 (3031S, 1:1000), anti-NFkB p65 antibody (6956S, 1:1000), anti-ATG5 antibody (12994S, 1:1000), anti-β-actin (3700S, 1:10000), anti-Flag-HRP (2044S, 1:20000) were from Cell Signaling Technology. All of the unprocessed scans of the blots were shown in the Source Data file.
Qin Y., Li B., Arumugam S., Lu Q., Mankash S.M., Li J., Sun B., Li J., Flavell R.A., Li H.B, & Ouyang X. (2021). m6A mRNA methylation-directed myeloid cell activation controls progression of NAFLD and obesity. Cell reports, 37(6), 109968.
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